Figure 1: Schematic representation of the four platelet-rich plasma (PRP) fractions obtained with the single (A and B) and double (C and D) centrifugation tube methods. (a) Sodium citrate tube containing whole blood. After centrifugation, whole blood was separated in several components: red cells (PCV), buffy coat (BC), and the PRP-A (50%) and PRP-B (50%) fractions. (b) A 10 mL sterile plastic tube containing PRP-A. After centrifugation, this hemoderivative was divided into PRP-C (25%) and PRP-D (75%) fractions.