Assessment of Pasteurella multocida A Lipopolysaccharide, as an Adhesin in an In Vitro Model of Rabbit Respiratory Epithelium

Figure 2

Simultaneous and sequential exposure to P. multocida and to its LPS and to P. multocida alone in nasal septa explants: LH and IIP. (a) Tissue cultures that were not exposed to LPS or P. multocida; ciliated cells and goblet cells were not labeled. (b) Tissues that were only exposed to P. multocida (thick arrow). LPS is visible within the cytoplasm of goblet cells (); faint LPS labeling lines the apical border of apparently ciliated cells that have lost their cilia; an apparent brown color is located intracytoplasmically in a nonidentifiable cell (thin arrow). (c) Simultaneous exposure to P. multocida LPS and P. multocida (). Most of the LPS labeling is located within the cytoplasm of goblet cells; several goblet cells are extruding their content along with LPS (arrow). (d) Tissues that were exposed to P. multocida LPS followed by P. multocida () 30 min later show a similar appearance and signs as in (c); note the clearer interface between LPS and the bacteria (thin arrows); GC: goblet cell. H&E: 100x.

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