Assessment of Pasteurella multocida A Lipopolysaccharide, as an Adhesin in an In Vitro Model of Rabbit Respiratory Epithelium

Table 1

Experimental protocol for exposing fetal rabbit nasal septa to P. multocida and LPS: IIP (indirect immunoperoxidase), LH (lectin histochemistry), TEM (transmission electron microscopy), and C (−) (negative control) [27, 28].

Treatment	⁢Negative control		P. multocida		LPS + P. multocida simultaneously	LPS + P. multocida 30 min later		P. multocida + LPS 30 min later	LPS
	⁢Treatment 1		Treatment 2		Treatment 3	Treatment 4		Treatment 5	Treatment 6
Technique	IIP-LH	TEM	IIP-LH	TEM	IIP-LH	IIP-LH	TEM	IIP-LH	TEM
Number of explants	7	3	7	3	7	7	3	7	3
Dose	MEM	MEM	1 × 10^8 cfu/mL	1 × 10^8 cfu/mL	10 µg/mL + 1 × 10^8 cfu/mL	10 µg/mL + 1 × 10^8 cfu/mL	10 µg/mL + 1 × 10^8 cfu/mL	1 × 10^8 cfu/mL + 10 µg/mL	10 µg/mL
Exposure time	2 h	2 h	2 h	2 h	2 h	2 h	2 h	2 h	2 h