Development and Evaluation of an Immuno-Capture Enzyme-Linked Immunosorbent Assay to Quantify the Mycoplasma capricolum subsp. capripneumoniae (Mccp) Protein in Contagious Caprine Pleuropneumonia (CCPP) Vaccine

Table 2

Combinations of unlabelled mAbs and HRP-labelled mAbs for ELISA on Mccp proteins.

mAbs	Mccp protein/PBS	HRP-labelled Mccp-25 (O.D value)	HRP-labelled Mccp-21 (O.D value)	HRP-labelled Mccp-16 (O.D value)
Unlabelled Mccp-16	Mccp protein	0.10	0.16	0.30
	PBS control	0.04	0.12	0.27
	Binding ratio	2	1	1
Unlabelled Mccp-21	Mccp protein	0.11	0.21	0.43
	PBS control	0.06	0.21	0.37
	Binding ratio	2	1	1
Unlabelled Mccp-25	Mccp protein	0.53	0.22	0.38
	PBS control	0.05	0.16	0.32
	Binding ratio	11	1	1

Cross-reacting these mAbs to HRP-labelled mAbs on Mccp protein showed that Mccp-25 mAb captured Mccp protein.