A 150 kDa Protein Derived from Bull Seminal Plasma Extended the Survival Time of Kacang Goat Sperm Stored at 5°C

Table 4

The average viability, motility, intactness of plasma membrane percentages, MDA concentration, and percentage of apoptotic sperm of Kacang goat sperm stored at 5°C for five days in skim milk-egg yolk extender with and without the addition of 150 kDa protein isolated from Simmental bull seminal plasma.

Parameters	Day 1	Day 2	Day 3	Day 4	Day 5
Viability (%)
T0	48.50 ± 0.40^Ac	45.25 ± 0.05^Bc	41.35 ± 0.05^Cc	33.15 ± 0.05^Dc	26.25 ± 0.20^Ec
TI	77.15 ± 0.25^Aa	75.30 ± 0.25^Ba	64.45 ± 0.55^Ca	54.25 ± 0.35^Da	34.15 ± 0.15^Ea
TII	59.10 ± 0.25^Ab	50.20 ± 0.25^Bb	45.40 ± 0.35^Cb	38.10 ± 0.45^Db	31.25 ± 0.40^Eb
Motility (%)
T0	46.50 ± 0.40^Ac	43.25 ± 0.25^Bc	40.25 ± 0.05^Cc	30.45 ± 0.05^Dc	23.35 ± 0.10^Ec
TI	74.05 ± 0.35^Aa	73.40 ± 0.05^Ba	62.45 ± 0.85^Ca	52.25 ± 0.40^Da	40.05 ± 0.15^Ea
TII	57.10 ± 0.35^Ab	48.20 ± 0.35^Bb	43.50 ± 0.75^Cb	36.10 ± 0.45^Db	28.25 ± 0.75^Eb
IPM (%)
T0	46.30 ± 0.30^Ac	41.15 ± 0.05^Bc	40.15 ± 0.05^Cc	32.25 ± 0.05^Dc	24.25^c ± 0.20^Ec
TI	75.15 ± 0.25^Aa	70.30 ± 0.25^Ba	62.15 ± 0.25^Ca	53.25 ± 0.35^Da	46.15^a± 0.15^Ea
TII	53.10 ± 0.25^Ab	46.20 ± 0.25^Bb	44.30 ± 0.15^Cb	37.10 ± 0.25^Db	30.10^b ± 0.30^Eb
MDA (ng/mL)
T0	2088.37 ± 35.18^Aa	2216.32 ± 37.70^Ba	2455.26 ± 37.75^Ca	2705.59 ± 50.75^Da	2980.50 ± 22.55^Ea
TI	1556.35 ± 40.20^Ac	1666.19 ± 33.20^Bc	1835.35 ± 25.19^Cc	1976.54 ± 34.23^Dc	2106.54 ± 30.35^Ea
TII	1845.20 ± 23.10^Ab	2052.28 ± 30.15^Bb	2150.73 ± 35.80^Cb	2530.42 ± 46.12^Db	2580.40 ± 12.10^Eb
Apoptosis
T0	4.25 ± 0.15^Ca	5.25 ± 0.55^Ba	5.75 ± 0.07^Ba	6.15 ± 0.05^Aa	6.25 ± 0.10^Aa
TI	2.40 ± 0.35^Cc	2.70 ± 0.25^Cc	3.35 ± 0.45^Bc	3.60 ± 0.25^Bc	4.15 ± 0.15^Ac
TII	3.70 ± 0.35^Cb	4.30 ± 0.15^Bb	5.05 ± 0.20^Ab	5.40 ± 0.15^Ab	5.50 ± 0.25^Ab

Different capital letter superscripts in the same row and lowercase letter superscripts in the same column indicate significant differences (). SM-EY: skim milk-egg yolk; T0: SM-EY extender without the protein; T1: SM-EY extender +12 μg protein/100 mL extender; T2: SM-EY extender +24 μg protein/100 mL extender. The parameters of the best quality semen are in bold. Values above the minimum progressive sperm motility (40%) for intracervical artificial insemination are indicated by italics.