Development and Validation of a Novel ELISA for the Specific Detection of Antibodies against Mycobacterium avium Subspecies paratuberculosis Based on a Chimeric Polyprotein

Table 1

Results of repeatability assessment of ten sera, with mean, standard deviation (SD), and coefficient of variation (CV; CV = SD/mean) for each sample from 30 independent runs carried out on different days.

Sample	Mean	SD	CV
POS1	0.623	0.086	0.13804173
POS2	0.715	0.091	0.12727273
POS3	0.554	0.067	0.12093863
POS4	0.469	0.103	0.2196162
POS5	0.41	0.092	0.22439024
POS6	0.596	0.097	0.16275168
NEG1	0.112	0.009	0.08035714
NEG2	0.135	0.019	0.14074074
NEG3	0.109	0.007	0.06422018
NEG4	0.198	0.041	0.20707071
BUFFER	0.042	0.001	0.02380952

POS1–POS6 are positive samples with a corrected optical density that ranges between 0.4 and 0.75; NEG1–NEG4 are negative samples with a corrected optical density <0.2. BUFFER (PBS (Phosphate-Buffered Saline) alone) sample was used as control.