Hindawi Publishing Corporation http://www.hindawi.com The latest articles from Hindawi Publishing Corporation © 2016 , Hindawi Publishing Corporation . All rights reserved. Influence of Physicochemical Aspects of Substratum Nanosurface on Bacterial Attachment for Bone Implant Applications Mon, 24 Oct 2016 09:20:13 +0000 http://www.hindawi.com/journals/jnt/2016/5026184/ Biofilm formation on implant materials is responsible for periprosthetic infections. Bacterial attachment is important as the first stage in biofilm formation. It is meaningful to understand the influence of nanostructured surface on bacterial attachment. This review discusses the influence of physicochemical aspects of substratum nanosurface on bacterial attachment. Kun Mediaswanti Copyright © 2016 Kun Mediaswanti. All rights reserved. Pilot Study on MAGE-C2 as a Potential Biomarker for Triple-Negative Breast Cancer Mon, 24 Oct 2016 09:16:25 +0000 http://www.hindawi.com/journals/dm/2016/2325987/ Objective. In the current study, we measured the expression status of melanoma antigen gene c2 (MAGE-C2) in triple-negative breast cancer (TNBC) and analyzed its prognostic with the clinical pathological features of patients with TNBC. Methods. The expressions statuses of MAGE-C2 were detected in TNBC tissues and paracarcinoma tissues by immunohistochemistry, reverse transcription-polymerase chain reaction (RT-PCR), and western blotting. Then, we investigated the relationship of MAGE-C2 expression status and clinicopathological parameters of TNBC patients by the chi-squared test. Finally, we discussed the relations of MAGE-C2 expression state and prognosis of patients with TNBC by Kaplan-Meier method and Cox proportional hazards model. Results. High MAGE-C2 expression was found in 38.18% (42/110) of TNBC tissues. In adjacent tissues it was 9.09% (10/110). High MAGE-C2 expression in TNBC patients was closely associated with lymph node status, tumor node metastasis (TNM) stage, and lymphovascular invasion (). TNBC patients with high MAGE-C2 expression had significantly shorter survival time than low expression patients. We also found that age, lymph node status, TNM stage, lymphovascular invasion, and MAGE-C2 expression status were closely associated with overall survival of TNBC patients (). Conclusion. High MAGE-C2 expression may serve as an independent prognostic factor for TNBC patients. Qian Zhao, Wen-ting Xu, and Tuluhong Shalieer Copyright © 2016 Qian Zhao et al. All rights reserved. Changing Clinical Presentation, Current Knowledge-Attitude-Practice, and Current Vision Related Quality of Life in Self-Reported Type 2 Diabetes Patients with Retinopathy in Eastern India: The LVPEI Eye and Diabetes Study Mon, 24 Oct 2016 09:16:23 +0000 http://www.hindawi.com/journals/joph/2016/3423814/ Purpose. To document the changing clinical presentation of diabetic retinopathy (DR) over a decade, the current knowledge-attitude-practice (KAP) of known type 2 diabetes mellitus (DM) patients, and the current vision related quality of life (VR-QOL) of patients with DR in a tertiary eye care center in Eastern India. Methods. Two hundred and forty patients with known type-2 DM were evaluated. The evaluation included status of DR (), KAP (), and VR-QOL (). International classification of DR was used in the study. The DR status was compared with another cohort () examined a decade earlier, in year 2001. The KAP-25 questions were designed after literature review. The National Eye Institute Visual Function Questionnaire (NEI-VFQ; including optional items) was validated by Rasch analysis. Both KAP and VR-QOL were analyzed according to degree of DR, duration of known DM, and educational qualification. Results. Average age of the current cohort () was 57.16 ± 9.03 years; there were 205 (85.4%) male patients and 143 (59.6%) patients had received less than graduate qualification. The mean duration of DM since diagnosis was 10 ± 7.8 months (range 8 months to 30 years); 118 (49.16%) patients had DR. In a decade time, 2001 to 2011, there was a change of retinopathy status at presentation (more often nonproliferative diabetic retinopathy, NPDR). One-third of NPDR patients had poor vision and half of them were hypertensive. KAP was better in patients with higher education and those having DR. VFQ score was higher in better seeing patients. Conclusion. Patients currently presenting at earlier stage of retinopathy are probably related to poor vision. Early detection and treatment of DR is likely to preserve and/or improve vision. Taraprasad Das, Batriti Wallang, Preeti Semwal, Soumyava Basu, Tapas R. Padhi, and Mohd Hasnat Ali Copyright © 2016 Taraprasad Das et al. All rights reserved. Transitional Remodeling of the Hepatic Extracellular Matrix in Alcohol-Induced Liver Injury Mon, 24 Oct 2016 09:12:53 +0000 http://www.hindawi.com/journals/bmri/2016/3162670/ Alcohol consumption is a common custom worldwide, and the toxic effects of alcohol on several target organs are well understood. The liver is the primary site of alcohol metabolism and is therefore the major target of alcohol toxicity. Alcoholic liver disease is a spectrum of disease states, ranging from simple steatosis (fat accumulation), to inflammation, and eventually to fibrosis and cirrhosis if untreated. The fibrotic stage of ALD is primarily characterized by robust accumulation of extracellular matrix (ECM) proteins (collagens) which ultimately impairs the function of the organ. The role of the ECM in early stages of ALD is poorly understood, but recent research has demonstrated that a number of changes in the hepatic ECM in prefibrotic ALD not only are present, but may also contribute to disease progression. The purpose of this review is to summarize the established and proposed changes to the hepatic extracellular matrix (ECM) that may contribute to earlier stages of ALD development and to discuss potential mechanisms by which these changes may mediate the progression of the disease. Lauren G. Poole and Gavin E. Arteel Copyright © 2016 Lauren G. Poole and Gavin E. Arteel. All rights reserved. A New Data Representation Based on Training Data Characteristics to Extract Drug Name Entity in Medical Text Mon, 24 Oct 2016 09:08:20 +0000 http://www.hindawi.com/journals/cin/2016/3483528/ One essential task in information extraction from the medical corpus is drug name recognition. Compared with text sources come from other domains, the medical text mining poses more challenges, for example, more unstructured text, the fast growing of new terms addition, a wide range of name variation for the same drug, the lack of labeled dataset sources and external knowledge, and the multiple token representations for a single drug name. Although many approaches have been proposed to overwhelm the task, some problems remained with poor F-score performance (less than 0.75). This paper presents a new treatment in data representation techniques to overcome some of those challenges. We propose three data representation techniques based on the characteristics of word distribution and word similarities as a result of word embedding training. The first technique is evaluated with the standard NN model, that is, MLP. The second technique involves two deep network classifiers, that is, DBN and SAE. The third technique represents the sentence as a sequence that is evaluated with a recurrent NN model, that is, LSTM. In extracting the drug name entities, the third technique gives the best F-score performance compared to the state of the art, with its average F-score being 0.8645. Mujiono Sadikin, Mohamad Ivan Fanany, and T. Basaruddin Copyright © 2016 Mujiono Sadikin et al. All rights reserved. Comparison between Manual and Automated Methods for Ki-67 Immunoexpression Quantification in Ameloblastomas Mon, 24 Oct 2016 09:07:23 +0000 http://www.hindawi.com/journals/acp/2016/7486989/ Ameloblastoma is a common and unpredictable odontogenic tumor with high relapse rates. Several studies assessing the proliferative capacity of these neoplasms have been published, mainly using the protein Ki-67. Cell counts must be completed to determine the cell proliferation rate. Multiple methods have been developed for this purpose. The most widely used method is the labeling index, which has undergone changes over time to better facilitate cell counting. Here, we compared manual cell counting methods with automated cell counting (ImmunoRatio) to determine the relative effectiveness of these methods. The results suggest that ImmunoRatio, a free software tool, may be highly advantageous and provide results similar to manual cell counting methods when used with the appropriate calibration. However, ImmunoRatio has flaws that may affect the labeling index results. Therefore, this automated cell counting method must be supplemented with manual cell counting methods. Rogelio González-González, Nelly Molina-Frechero, Ramón G. Carreón-Burciaga, Sandra López-Verdín, Carlos Robles-Bonilla, Vanesa Pereira-Prado, and Ronell Bologna-Molina Copyright © 2016 Rogelio González-González et al. All rights reserved. Phytochemical Content and Pharma-Nutrition Study on Eleutherococcus senticosus Fruits Intractum Mon, 24 Oct 2016 09:02:48 +0000 http://www.hindawi.com/journals/omcl/2016/9270691/ In the past two decades public interest in herbal products has increased significantly in Europe, especially in the plant-based products from non-European traditions. Eleutherococcus senticosus has been used for the treatment of inflammatory diseases, anemia, and rheumatoid arthritis. The Eleutherococcus senticosus fruits intractum was examined for the content of phenolic acids (LC-ESI-MS/MS), minerals (AAS), TPC, and TFC (spectrophotometric assay). The antioxidant activity was determined using free radical scavenging assay and TLC-DB-DPPH∗ dot-blot test. An anti-Hyal activity was evaluated by the spectrophotometric assay method. Cytotoxicity towards HL-60, HL-60/MX1, HL-60/MX2, CEM/C1, and CCRF/CEM leukemic cell lines was done using trypan blue test. Among eight phenolic acids, trans-caffeic acid was found in the largest amount (41.2 mg/g DE). The intractum presented a high amount of macroelements (Ca, Mg, K; 1750, 1300, and 21000 mg/kg) and microelements (Fe, Mn; 32.7, 54.3 mg/kg), respectively. The content of TPC and TFC was 130 and 92 mg/g DE, respectively. The intractum showed anti-Hyal activity (2.16–60%) and an antioxidant capacity (EC50; 52 g/mL). The intractum most strongly inhibited the growth of HL-60, HL-60/MX1, and CCRF/CEM. A better understanding of the intractum health benefits is important in order to increase its utility and enrich dietary sources of health promoting compounds. Daniel Załuski, Marta Olech, Agnieszka Galanty, Robert Verpoorte, Rafał Kuźniewski, Renata Nowak, and Anna Bogucka-Kocka Copyright © 2016 Daniel Załuski et al. All rights reserved. A Case of a Chronic Pancreatic Pseudocyst Causing Atraumatic Splenic Rupture without Evidence of Acute Pancreatitis Mon, 24 Oct 2016 08:55:43 +0000 http://www.hindawi.com/journals/cris/2016/2192943/ Atraumatic splenic rupture is a rare complication of a pancreatic pseudocyst (PP), described in the setting of chronic pancreatitis. There is common understanding, within the literature, that an inflammatory process at the tail of the pancreas may disrupt the spleen and result in such splenic complications. The authors present a case report of a 29-year-old male with a PP, associated with chronic pancreatitis. The patient had a history of excessive alcohol intake and presented to the emergency department with a short history of abdominal pain and vomiting. He denied any significant history of trauma and serum amylase levels were normal. An admission computed tomography (CT) scan of the abdomen confirmed the presence of a PP in direct contact with the spleen. The CT also demonstrated a heterogenous hypodense area of the splenic hilum, along with perisplenic fluid. The patient was admitted for observation. His abdominal pain progressed, and he became haemodynamically unstable. An emergency ultrasound scan (USS) at this time revealed intra-abdominal haemorrhage. A subsequent CT confirmed splenic rupture, which was managed surgically with a full recovery. Few such cases are documented within the literature and more understanding of preempting such events is needed. P. Moori, E. J. Nevins, T. Wright, C. Bromley, and Y. Rado Copyright © 2016 P. Moori et al. All rights reserved. Addition and Subtraction Theory of TCM Using Xiao-Chaihu-Decoction and Naturopathy in Predicting Survival Outcomes of Primary Liver Cancer Patients: A Prospective Cohort Study Mon, 24 Oct 2016 08:54:27 +0000 http://www.hindawi.com/journals/ecam/2016/4723530/ To investigate the therapeutic effect of combined Xiao-Chaihu-Decoction and naturopathic medicine therapy on survival outcomes of patients’ PLC. In XCHD group (), patients were treated with Xiao-Chaihu-Decoction in accordance with the addition and subtraction theory of TCM; in NM group (), patients were managed by naturopathic medicine; in combined group (), the same volume of Xiao-Chaihu-Decoction combined with naturopathic medicine procedures was applied. There were no evident statistical differences of age, gender, KPS score, body weight, smoking status, AFP levels, HbsAg status, TBIL levels, tumor diameters, and numbers among different groups, showing comparability among groups. No significant difference was found regarding the total remission rate and stability rate of tumors in patients treated by Xiao-Chaihu-Decoction and naturopathic medicine, except the combined therapy. KPS scores were significantly improved after treatment among groups. After treatment, 52.8% cases maintained a stable or slight increase in weight, of which 42.1%, 48.3%, and 70.0% cases maintained weight stably in the XCHD group, NM group, and combined treatment group, respectively. Xiao-Chaihu-Decoction associated with naturopathy may predict improved prognostic outcomes in PLC patients, along with improved remission and stability rates, increased KPS scores, and stable weight maintenance. Min Dai, Yue-Wu Yang, Wen-Hai Guo, Feng-Lin Wang, Ge-Min Xiao, Yang-Mei Li, and Hong-Zhi Yang Copyright © 2016 Min Dai et al. All rights reserved. Massive Upper Gastrointestinal Bleeding Caused by Diffuse Large B-Cell Lymphoma Mon, 24 Oct 2016 08:53:53 +0000 http://www.hindawi.com/journals/crigm/2016/5079709/ Massive upper gastrointestinal bleeding is a life-threatening emergency which needs urgent intervention. Hematological malignancies are very rare causes of this type of bleeding and they usually originate from duodenum. In this case we present a gastric diffuse large B-cell lymphoma (DLBCL) causing massive upper gastrointestinal system bleeding. A 77-year-old male patient was admitted to emergency clinic with hematemesis and hematochezia. In physical examination patient was pale and sweaty; his vitals were unstable with a heart rate of 110 per minute and a blood pressure of 90/50 mmHg. His hemoglobin level was found 7.5 g/dL and he was transfused with one unit of packed red blood cells. After his vitals were normalized, gastroscopy was performed showing mosaic pattern in corpus and antrum mucosa and multiple ulcers in various sizes, largest being approximately 2 cm in diameter, higher than mucosa covered with exude mostly on corpus and large curvature. Biopsy results were reported as DLBCL. Gastric mucosa is involved in most of the DLBCL cases. Although not listed as a common cause of massive gastrointestinal bleeding DLBCL can cause life-threatening situations mostly because of its malignant nature. O. Telci Caklili, H. H. Mutlu, Y. Colak, E. Ozturk, D. Kosemetin Dover, and I. Tuncer Copyright © 2016 O. Telci Caklili et al. All rights reserved. Improving Localization in Wireless Sensor Network Using Fixed and Mobile Guide Nodes Mon, 24 Oct 2016 08:44:51 +0000 http://www.hindawi.com/journals/js/2016/6385380/ Wireless sensor network contains very large number of tiny sensors; some nodes with known position are recognized as guide nodes. Other nodes with unknown position are localized by guide nodes. This article uses the combination of fixed and mobile guide nodes in wireless network localization. So nearly 20% of nodes are fixed guide nodes and three nodes are intended as mobile guide nodes. To evaluate the proficiency, the proposed algorithm has been successfully studied and verified through simulation. Low cost, high accuracy, and low power consumption of nodes and complete coverage are the benefits of this approach and long term in localization is the disadvantage of this method. R. Ahmadi, G. Ekbatanifard, A. Jahangiry, and M. Kordlar Copyright © 2016 R. Ahmadi et al. All rights reserved. Efficient Regularized Regression with Penalty for Variable Selection and Network Construction Mon, 24 Oct 2016 08:09:34 +0000 http://www.hindawi.com/journals/cmmm/2016/3456153/ Variable selections for regression with high-dimensional big data have found many applications in bioinformatics and computational biology. One appealing approach is the regularized regression which penalizes the number of nonzero features in the model directly. However, it is well known that optimization is NP-hard and computationally challenging. In this paper, we propose efficient EM (EM) and dual EM (DEM) algorithms that directly approximate the optimization problem. While EM is efficient with large sample size, DEM is efficient with high-dimensional () data. They also provide a natural solution to all    problems, including lasso with and elastic net with . The regularized parameter can be determined through cross validation or AIC and BIC. We demonstrate our methods through simulation and high-dimensional genomic data. The results indicate that has better performance than lasso, SCAD, and MC+, and with AIC or BIC has similar performance as computationally intensive cross validation. The proposed algorithms are efficient in identifying the nonzero variables with less bias and constructing biologically important networks with high-dimensional big data. Zhenqiu Liu and Gang Li Copyright © 2016 Zhenqiu Liu and Gang Li. All rights reserved. Cannabis Use as Risk or Protection for Type 2 Diabetes: A Longitudinal Study of 18 000 Swedish Men and Women Mon, 24 Oct 2016 07:43:37 +0000 http://www.hindawi.com/journals/jdr/2016/6278709/ Aims. Whether or not cannabis use may increase or decrease the risk of type 2 diabetes is not clear. We analyzed the association between cannabis and subsequent type 2 diabetes and if a potential positive or reverse association persisted after controlling for potential confounders. Methods. In this population-based cohort study, 17,967 Swedish men and women (aged 18–84 years), who answered an extensive questionnaire in 2002 (including questions on cannabis use), were followed up for new cases of type 2 diabetes () by questionnaire (in 2010) and in health registers during 2003–2011. Odds ratios (ORs) with 95% CIs were estimated in a multiple logistic regression analysis. Potential confounders included age, sex, BMI, physical inactivity, smoking, alcohol use, and occupational position. Results. The crude association showed that cannabis users had a reduced risk of type 2 diabetes OR = 0.68 (95% CIs: 0.47–0.99). However, this inverse association attenuated to OR = 0.94 (95% CIs: 0.63–1.39) after adjusting for age. Conclusions. The present study suggests that there is no association between cannabis use and subsequent type 2 diabetes after controlling for age. To make more robust conclusions prospective studies, with longer periods of follow-up and more detailed information about cannabis use, are needed. A. K. Danielsson, A. Lundin, A. Yaregal, C. G. Östenson, P. Allebeck, and E. E. Agardh Copyright © 2016 A. K. Danielsson et al. All rights reserved. Effect of Three-Dimensional Printed Personalized Moisture Chamber Spectacles on the Periocular Humidity Mon, 24 Oct 2016 07:42:40 +0000 http://www.hindawi.com/journals/joph/2016/5039181/ Purpose. To assess the effect of three-dimensional (3D) printed personalized moisture chamber spectacles (PMCS) on the periocular humidity. Methods. Facial computed tomography (CT) scanning was conducted on 10 normal subjects. PMCS was designed based on volume rendered CT images and produced using a 3D printer. Periocular humidity of PMCS and commercially available uniformed moisture chamber spectacles (UMCS) were measured for 30 minutes via microhydrometer. Results. The mean ambient humidity was %. The mean periocular humidity was % in PMCS and % in UMCS. The difference was significant (). Additionally, PMCS always demonstrated lower humidity than dew points. Conclusion. PMCS made by 3D printer provides appropriate fitness for the semiclosed humid chamber. PMCS showed higher performance than UMCS. The wearing of PMCS would be an effective method to provide high enough periocular humidity in low humidity environment. Chan Hee Moon, Jae Yong Kim, Myoung Joon Kim, Hungwon Tchah, Byeong Gak Lim, and Jin Kwon Chung Copyright © 2016 Chan Hee Moon et al. All rights reserved. Effect of an Albumin-Coated Mesoporous Silicon Nanoparticle Platform for Paclitaxel Delivery in Human Lung Cancer Cell Line A549 Mon, 24 Oct 2016 07:42:34 +0000 http://www.hindawi.com/journals/jnm/2016/4086456/ Albumin-coated paclitaxel-mesoporous silicon nanoparticles (APMSN) were prepared to improve the anticancer effect in lung cancer by means of regulating the dissolution rate of paclitaxel (PTX). PTX was absorbed into the mesoporous structure of mesoporous silicon nanoparticles (MSN), which was defined as PMSN. PTX was proved to exist in an amorphous state in PMSN, which increased the dissolution rate of PTX. Albumin was coated on the surface of MSN to form AMSN; AMSN and PTX were mixed to form APMSN in order to achieve sustained release of PTX. Then, it was found that APMSN had more significant antiproliferate effects and induced more apoptotic proportion in comparison with PTX in A549 cells. Furthermore, the absorption mechanism of APMSN into A549 cells was investigated. Transmission electron microscopy (TEM) and laser scanning confocal microscopy (LSCM) showed that APMSN could cross the cell membrane and was taken into the cytoplasm quickly. Taken together, our results demonstrate that AMSN carriers have potential as nanodrug delivery systems in the treatment of lung cancer. Yu Gao, Xiaofang Che, Chunlei Zheng, Kezuo Hou, Xiujuan Qu, Yunpeng Liu, and Zhitu Zhu Copyright © 2016 Yu Gao et al. All rights reserved. Anterior Pituitary Aplasia in an Infant with Ring Chromosome 18p Deletion Mon, 24 Oct 2016 07:41:40 +0000 http://www.hindawi.com/journals/crie/2016/2853178/ We present the first reported case of an infant with 18p deletion syndrome with anterior pituitary aplasia secondary to a ring chromosome. Endocrine workup soon after birth was reassuring; however, repeat testing months later confirmed central hypopituitarism. While MRI reading initially indicated no midline defects, subsequent review of the images confirmed anterior pituitary aplasia with ectopic posterior pituitary. This case demonstrates how deletion of genetic material, even if resulting in a chromosomal ring, still results in a severe syndromic phenotype. Furthermore, it demonstrates the necessity of close follow-up in the first year of life for children with 18p deletion syndrome and emphasizes the need to verify radiology impressions if there is any doubt as to the radiologic findings. Edward J. Bellfield, Jacqueline Chan, Sarah Durrin, Valerie Lindgren, Zohra Shad, and Claudia Boucher-Berry Copyright © 2016 Edward J. Bellfield et al. All rights reserved. Moyamoya Disease with Coexistent Hypertriglyceridemia in Pediatric Patient Mon, 24 Oct 2016 07:41:05 +0000 http://www.hindawi.com/journals/crie/2016/7974182/ Moyamoya disease is a rare chronic and progressive cerebrovascular disease of the arteries of the circle of Willis that can affect children and adults. It has been associated with multiple diseases, including immunologic, like Graves’ disease, diabetes mellitus, and SLE. Hyperlipidemia has been recognized in patients with Moyamoya disease with an incidence of 27–37%. However, no case in pediatric patients has been reported of the coexistence of Moyamoya disease and hyperlipidemia. Here we present a case of a 9-year-old female diagnosed with Moyamoya disease after a stroke with incidental finding of familial hypercholesterolemia. This finding will make our patient a very unique case, since there has not been any reporting of Moyamoya disease and hypercholesterolemia association. Jacqueline Chan, Fabiola D’Ambrosio Rodriguez, Deepank Sahni, and Claudia Boucher-Berry Copyright © 2016 Jacqueline Chan et al. All rights reserved. Minimally Invasive Treatment of Mirizzi Syndrome, a Rare Cause of Cholestasis in Childhood Mon, 24 Oct 2016 07:39:24 +0000 http://www.hindawi.com/journals/cripe/2016/8940570/ Mirizzi syndrome is the compressive blockage of the cystic or choledochal duct caused by a biliary stone occupying the cystic canal or Hartmann’s pouch. This occurrence is rare and, in English literature, three cases defined in children have been observed. In order to draw attention to this rare occurrence, we preferred a 14-year-old male patient with Mirizzi syndrome. In this case, ERCP was performed preoperatively and the diagnosis was carried out with the help of clear visualisation and identification of the tissue structures as well as the stent placed in bile duct; so we protected the patient from the possible iatrogenic injury occurring during surgery. Ahmet Ali Tuncer, Sezgin Yilmaz, Mustafa Yavuz, and Salih Çetinkurşun Copyright © 2016 Ahmet Ali Tuncer et al. All rights reserved. Initiation of Failure for Masonry Subject to In-Plane Loads through Micromechanics Mon, 24 Oct 2016 07:38:25 +0000 http://www.hindawi.com/journals/mse/2016/2959038/ A micromechanical procedure is used in order to evaluate the initiation of damage and failure of masonry with in-plane loads. Masonry material is viewed as a composite with periodic microstructure and, therefore, a unit cell with suitable boundary conditions is assumed as a representative volume element of the masonry. The finite element method is used to determine the average stress on the unit cell corresponding to a given average strain prescribed on the unit cell. Finally, critical curves representing the initiation of damage and failure in both clay brick masonry and adobe masonry are provided. V. P. Berardi Copyright © 2016 V. P. Berardi. All rights reserved. β-Catenin Expression Negatively Correlates with WIF1 and Predicts Poor Clinical Outcomes in Patients with Cervical Cancer Mon, 24 Oct 2016 07:35:31 +0000 http://www.hindawi.com/journals/bmri/2016/4923903/ Aberrant activation of the canonical Wnt pathway plays a significant role in cervical cancer (CC). However, limited data show the correlation between the cancer clinicopathological characteristics and the key molecules such as β-catenin and Wnt inhibitory factor 1 (WIF1). In this study, β-catenin and WIF1 expression were analyzed by immunohistochemistry for 196 patients with CC, 39 with cervical intraepithelial neoplasia (CIN), and 41 with normal cervical epithelium (NCE). Significant overexpression of β-catenin was detected in CC (67.9%) when compared to CIN (43.6%) or NCE (34.1%), , while low WIF1 expression was detected in CC (24.0%) when compared to CIN (59.0%) or NCE (58.5%), . Negative correlation was shown between β-catenin and WIF1 expression (, ). In addition, multivariate analysis revealed that both lymph node metastasis and β-catenin expression were the independent prognostic factors not only for disease-free survival (HR = 5.029, ; HR = 2.588, , resp.), but also for overall survival (HR = 5.058, ; HR = 2.873, , resp.). Our findings indicate that, besides lymph node metastasis, β-catenin expression may also be a poor prognostic factor for CC while WIF1 could be a potential drug target for treatment of advanced CC. Jinxiao Liang, Hui Zhou, Yongpai Peng, Xiaofei Xie, Ruixin Li, Yunyun Liu, Qingsheng Xie, and Zhongqiu Lin Copyright © 2016 Jinxiao Liang et al. All rights reserved. State of the Art, Unresolved Issues, and Future Research Directions in the Fight against Hepatitis C Virus: Perspectives for Screening, Diagnostics of Resistances, and Immunization Mon, 24 Oct 2016 07:33:56 +0000 http://www.hindawi.com/journals/jir/2016/1412840/ Hepatitis C virus (HCV) still represents a major public health threat, with a dramatic burden from both epidemiological and clinical points of view. New generation of direct-acting antiviral agents (DAAs) has been recently introduced in clinical practice promising to cure HCV and to overcome the issues related to the interferon-based therapies. However, the emergence of drug resistance and the suboptimal activity of DAAs therapies against diverse HCV genotypes have been observed, determining treatment failure and hampering an effective control of HCV spread worldwide. Moreover, these treatments remain poorly accessible, particularly in low-income countries. Finally, effective screening strategy is crucial to early identifying and treating all HCV chronically infected patients. For all these reasons, even though new drugs may contribute to impacting HCV spread worldwide a preventive HCV vaccine remains a cornerstone in the road to significantly reduce the HCV spread globally, with the ultimate goal of its eradication. Advances in molecular vaccinology, together with a strong financial, political, and societal support, will enable reaching this fundamental success in the coming years. In this comprehensive review, the state of the art about these major topics in the fight against HCV and the future of research in these fields are discussed. Cecilia Trucchi, Andrea Orsi, Cristiano Alicino, Laura Sticchi, Giancarlo Icardi, and Filippo Ansaldi Copyright © 2016 Cecilia Trucchi et al. All rights reserved. Conditioning with Fludarabine-Busulfan versus Busulfan-Cyclophosphamide Is Associated with Lower aGVHD and Higher Survival but More Extensive and Long Standing Bone Marrow Damage Mon, 24 Oct 2016 07:30:47 +0000 http://www.hindawi.com/journals/bmri/2016/3071214/ Acute graft-versus-host disease (aGVHD) is a major complication of allogeneic hematopoietic stem cell transplantation (allo-HSCT) and a major cause of nonrelapse mortality after allo-HSCT. A conditioning regimen plays a pivotal role in the development of aGVHD. To provide a platform for studying aGVHD and evaluating the impact of different conditioning regimens, we established a murine aGVHD model that simulates the clinical situation and can be conditioned with Busulfan-Cyclophosphamide (Bu-Cy) and Fludarabine-Busulfan (Flu-Bu). In our study, BALB/c mice were conditioned with Bu-Cy or Flu-Bu and transplanted with bone marrow cells and splenocytes from either allogeneic (C57BL/6) or syngeneic (BALB/c) donors. The allogeneic recipients conditioned with Bu-Cy had shorter survivals (), more severe clinical manifestations, and higher hepatic and intestinal pathology scores, associated with increased INF-γ expression and diminished IL-4 expression in serum, compared to allogeneic recipients conditioned with Flu-Bu. Moreover, higher donor-derived T-cell infiltration and severely impaired B-cell development were seen in the bone marrow of mice, exhibiting aGVHD and conditioned with Flu-Bu. Our study showed that the conditioning regimen with Bu-Cy resulted in more severe aGVHD while the Flu-Bu regimen was associated with more extensive and long standing bone marrow damage. Xin He, YongBin Ye, XiaoJun Xu, Jing Wang, YuXian Huang, GuangYang Weng, MingWan Zhang, and KunYuan Guo Copyright © 2016 Xin He et al. All rights reserved. Climate Change and Fruit-Picking Tourism: Impact and Adaptation Mon, 24 Oct 2016 07:28:27 +0000 http://www.hindawi.com/journals/amete/2016/9783646/ The purpose of this work is to present phenology as a valid indicator and methodology for monitoring and assessing the impact of climate change on plant-based tourist activities. Fruit-picking has become a popular rural tourism activity worldwide. However, fruit maturity dates (FMD) have been affected by climate change (CC), which has in turn profoundly affected fruit-picking tourism activities (FPTA). In this paper, phenological data on the FMD for 45 types of plants in 1980–2012, dates for more than 200 fruit-picking festivals, and data on monthly average air temperature in 1980–2013 were used to assess the impact of CC on FPTA by wavelet and correlation analyses. The findings indicated that the study area had been significantly affected by CC. Prevailing temperatures at one or three months prior have a decisive influence on FMD. Among the 11 plants directly related to FPTA, the FMD of four were significantly advanced, while 6-7 were significantly delayed owning to increased temperature. Of the 11 FPTA, only two had realized the impact of CC and had adjusted festival opening dates based on dynamic changes. However, a considerable number of festival activities remained fixed or scheduled on the weekends. Jun Liu, Fan Chen, Quansheng Ge, and Yunyun Li Copyright © 2016 Jun Liu et al. All rights reserved. Full-Scale Experimental Validation of Large-Eddy Simulation of Wind Flows over Complex Terrain: The Bolund Hill Mon, 24 Oct 2016 07:28:14 +0000 http://www.hindawi.com/journals/amete/2016/9232759/ Numerical simulation of local atmospheric flows around a complex topography is important for many wind energy applications, as it can help in locating and optimizing wind farms. The aim of this paper is to simulate the atmospheric flows over the challenging and complex site of Bolund Hill (in Denmark) using Large-Eddy Simulations (LES) and to validate the simulation methodology against full-scale measurements. Wind prediction over a potential inland wind park is the main application of the validated LES methodology. For the first time, LES-based results from more than one wind direction of the Bolund case are reported and analyzed in detail herein. The LES results from each direction are compared with the Bolund field data in a quantitative way, for example, by using simulation error. According to the results comparison, it is found that the LES model provides by far the most accurate prediction for turbulent kinetic energy. The simulation error of this LES model for predicting turbulent kinetic energy is 19% smaller than that of all other models, whether experimental or numerical, employed previously over Bolund Hill. Ashvinkumar Chaudhari, Antti Hellsten, and Jari Hämäläinen Copyright © 2016 Ashvinkumar Chaudhari et al. All rights reserved. Virtual Correlations in Single Qutrit Mon, 24 Oct 2016 07:26:56 +0000 http://www.hindawi.com/journals/amp/2016/7213197/ We construct the positive invertible map of the mixed states of a single qutrit onto the antisymmetrized bipartite qutrit states (quasifermions). It is shown that using this one-to-one correspondence between qutrit states and states of two three-dimensional quasifermions one may attribute hidden entanglement to a single mixed state of qutrit. Alexey A. Strakhov and Vladimir I. Man’ko Copyright © 2016 Alexey A. Strakhov and Vladimir I. Man’ko. All rights reserved. Multiphase Return Trajectory Optimization Based on Hybrid Algorithm Mon, 24 Oct 2016 07:18:40 +0000 http://www.hindawi.com/journals/mpe/2016/6257050/ A hybrid trajectory optimization method consisting of Gauss pseudospectral method (GPM) and natural computation algorithm has been developed and utilized to solve multiphase return trajectory optimization problem, where a phase is defined as a subinterval in which the right-hand side of the differential equation is continuous. GPM converts the optimal control problem to a nonlinear programming problem (NLP), which helps to improve calculation accuracy and speed of natural computation algorithm. Through numerical simulations, it is found that the multiphase optimal control problem could be solved perfectly. Yi Yang and Ying Nan Copyright © 2016 Yi Yang and Ying Nan. All rights reserved. A Note on First Passage Functionals for Lévy Processes with Jumps of Rational Laplace Transforms Mon, 24 Oct 2016 07:18:39 +0000 http://www.hindawi.com/journals/aaa/2016/5914657/ This paper investigates the two-sided first exit problem for a jump process having jumps with rational Laplace transform. The corresponding boundary value problem is solved to obtain an explicit formula for the first passage functional. Also, we derive the distribution of the first passage time to two-sided barriers and the value at the first passage time. Djilali Ait-Aoudia Copyright © 2016 Djilali Ait-Aoudia. All rights reserved. Corrigendum to “Sphingosine-1-Phosphate Signaling in Immune Cells and Inflammation: Roles and Therapeutic Potential” Mon, 24 Oct 2016 07:16:11 +0000 http://www.hindawi.com/journals/mi/2016/2856829/ Masayo Aoki, Hiroaki Aoki, Rajesh Ramanathan, Nitai C. Hait, and Kazuaki Takabe Copyright © 2016 Masayo Aoki et al. All rights reserved. Management Strategy in Non-Limb-Threatening Acute Ischaemia of Limbs: Should We Rethink? Mon, 24 Oct 2016 07:09:45 +0000 http://www.hindawi.com/journals/crivam/2016/8146295/ The Society of Vascular Surgery and the International Society of Cardiovascular Surgery identify three types of acute limb ischaemia to inform prognosis and management. Type 1 limb ischaemia is non-limb-threatening and is currently managed conservatively. We describe three cases of Type 1 limb ischaemia with femoropopliteal occlusion that were managed differently. The first case was initially managed conservatively but resulted in an adverse outcome following worsening of ischaemia. Overall, the cases managed with earlier intervention had good outcomes suggesting that conservative management alone may not be sufficient despite resolution of symptoms. The trend in other vessel diseases such as NSTEMI and TIA is towards earlier intervention, for example, PCI and CEA. It is likely that acute limb ischaemia has a similar natural history to these conditions. It is time to consider earlier revascularisation in selected patients with non-limb-threatening ischaemia. Syed M. Asim Hussain and Thomas Joseph Copyright © 2016 Syed M. Asim Hussain and Thomas Joseph. All rights reserved. Investigation of Shadowing Effects in Typical Propagation Scenarios for High-Speed Railway at 2350 MHz Mon, 24 Oct 2016 07:09:08 +0000 http://www.hindawi.com/journals/ijap/2016/8782671/ Based on realistic measurements in China, shadowing characteristics at the frequency of 2350 MHz were investigated in typical High-Speed Railway environments. After confirming that the measured shadowing satisfies wide-sense stationarity (assessed via the reverse arrangement test method), we quantify the shadowing correlation. Three types of correlation models are compared for the shadowing characterization, and the Normalized Mean Square Error is used to determine the best matching model: a single decaying exponential function. Decorrelation distances were found to be 11.9 m, 17.7 m, and 8.3 m in our three HSR scenarios, respectively. The results should be useful for the evaluation and verification of wireless communication in High-Speed Railway scenarios. Liu Liu, Cheng Tao, David W. Matolak, Tao Zhou, and Houjin Chen Copyright © 2016 Liu Liu et al. All rights reserved. Short-Term Efficacy of Laparoscopic Treatment for Colorectal Cancer in Patients with Schistosomiasis Japonica Mon, 24 Oct 2016 07:08:23 +0000 http://www.hindawi.com/journals/grp/2016/8357025/ Introduction. Schistosomiasis is associated with numerous complications such as thrombocytopenia, liver cirrhosis, portal hypertension, and colitis. To the best of our knowledge, the feasibility and outcomes of laparoscopic colorectal surgery in patients with schistosomiasis have not yet been studied. Methods. In this study, the data of 280 patients with colorectal carcinoma along with schistosomiasis japonica infection who underwent laparoscopic or open colorectal surgery were retrospectively analyzed. Preoperative data, operative data, pathological outcomes, postoperative complications, and recovery were compared between patients in the laparoscopic (LAC) and open (OC) groups. Results. There were no significant differences in the preoperative data between the groups. However, fewer postoperative complications, especially severe hypoproteinemia, early postoperative feeding, and shorter postoperative hospital stay, were observed in patients in the LAC group (). The mean operative time was higher in the LAC group (180 min versus 158 min; ), while the mean blood loss was similar (95 mL versus 108 mL; ) between groups. The mean number of lymph nodes harvested was also similar in both groups (15 versus 16; ). Conclusion. Laparoscopic surgery for colorectal cancer is safe in patients with schistosomiasis japonica and has better short-term outcomes than open surgery. Zhu Yi, Jiang Hong-Gang, Chen Zhi-Heng, and Lu Bo-Hao Copyright © 2016 Zhu Yi et al. All rights reserved. Comment on “Correlation between Porosity and Electrical-Mechanical Properties of Carbon Nanotube Buckypaper with Various Porosities” Mon, 24 Oct 2016 07:00:07 +0000 http://www.hindawi.com/journals/jnm/2016/1592161/ Amit Rawal, Ákos Kukovecz, and Vijay Kumar Copyright © 2016 Amit Rawal et al. All rights reserved. Privacy Models in Wireless Sensor Networks: A Survey Mon, 24 Oct 2016 06:55:32 +0000 http://www.hindawi.com/journals/js/2016/4082084/ Wireless Sensor Networks (WSNs) are attracting attention from the research community. One of the key issues is to provide them with privacy protection. In recent years, a huge amount of contributions has been focused on this area. Surveys and literature reviews have also been produced to give a systematic view of the different approaches taken. However, no previous work has focused on privacy models, that is, the set of assumptions made to build the approach. In particular, this paper focuses on this matter by studying 41 papers of the last 5 years. We highlight the great differences appearing among related papers that could make them incompatible to be applied simultaneously. We propose a set of guidelines to build comprehensive privacy models so as to foster their comparability and suitability analysis for different scenarios. J. M. de Fuentes, L. González-Manzano, and O. Mirzaei Copyright © 2016 J. M. de Fuentes et al. All rights reserved. Effects of a Mixed Zone on TGO Displacement Instabilities of Thermal Barrier Coatings at High Temperature in Gas-Cooled Fast Reactors Mon, 24 Oct 2016 06:52:51 +0000 http://www.hindawi.com/journals/stni/2016/9071237/ Thermally grown oxide (TGO), commonly pure α-Al2O3, formed on protective coatings acts as an insulation barrier shielding cooled reactors from high temperatures in nuclear energy systems. Mixed zone (MZ) oxide often grows at the interface between the alumina layer and top coat in thermal barrier coatings (TBCs) at high temperature dwell times accompanied by the formation of alumina. The newly formed MZ destroys interface integrity and significantly affects the displacement instabilities of TGO. In this work, a finite element model based on material property changes was constructed to investigate the effects of MZ on the displacement instabilities of TGO. MZ formation was simulated by gradually changing the metal material properties into MZ upon thermal cycling. Quantitative data show that MZ formation induces an enormous stress in TGO, resulting in a sharp change of displacement compared to the alumina layer. The displacement instability increases with an increase in the MZ growth rate, growth strain, and thickness. Thus, the formation of a MZ accelerates the failure of TBCs, which is in agreement with previous experimental observations. These results provide data for the understanding of TBC failure mechanisms associated with MZ formation and of how to prolong TBC working life. Jian Wang, Jun Ding, Kun Song, Song Chen, and Xia Huang Copyright © 2016 Jian Wang et al. All rights reserved. Cystatin C Levels in Middle-Aged Patients with Obstructive Sleep Apnea Syndrome Mon, 24 Oct 2016 06:50:35 +0000 http://www.hindawi.com/journals/pm/2016/8081723/ Background. Obstructive sleep apnea syndrome (OSAS) is associated with systemic inflammation and increased risk of cardiovascular and chronic kidney disease. Cystatin C (Cyst C) is a novel biomarker of both latent renal damage and cardiovascular disease. Aim of the study was to measure serum levels of Cyst C, as well as IL-8 and CRP, in otherwise healthy OSAS patients. Methods. 84 individuals examined with polysomnography for OSAS symptoms without known comorbidities were prospectively recruited. Results. According to apnea hypopnea index (AHI) subjects were divided in two groups: OSAS group (AHI > 5/hour, ) and controls (AHI < 5/hour, ), which were age- and BMI-matched. Cyst C levels were higher in OSAS patients versus controls ( versus  ng/mL, resp.; ) while serum IL-8 and CRP levels did not differ significantly. Positive correlation was found between Cyst C levels and respiratory disturbance index (RDI) (, ) and percentage of time with oxygen saturation <90% (, ) and negative correlation was found between Cyst C levels and average oxygen saturation during sleep (, ). After adjustment for age and BMI, RDI was the only independent predictor of Cyst C levels (β = 0.256, ). Conclusion. Cyst C serum levels are increased in OSAS patients without comorbidities, suggesting an increased renal and cardiovascular disease risk. Kostas Archontogeorgis, Evangelia Nena, Christina Tsigalou, Athanasios Voulgaris, Maria Xanthoudaki, Marios Froudarakis, and Paschalis Steiropoulos Copyright © 2016 Kostas Archontogeorgis et al. All rights reserved. Observation of Dissipative Bright Soliton and Dark Soliton in an All-Normal Dispersion Fiber Laser Mon, 24 Oct 2016 06:26:46 +0000 http://www.hindawi.com/journals/ijo/2016/3946525/ This paper proposes a novel way for controlling the generation of the dissipative bright soliton and dark soliton operation of lasers. We observe the generation of dissipative bright and dark soliton in an all-normal dispersion fiber laser by employing the nonlinear polarization rotation (NPR) technique. Through adjusting the angle of the polarizer and analyzer, the mode-locked and non-mode-locked regions can be obtained in different polarization directions. Numerical simulation shows that, in an appropriate pump power range, the dissipative bright soliton and dark soliton can be generated simultaneously in the mode-locked and non-mode-locked regions, respectively. If the pump power exceeds the top limit of this range, only dissipative soliton will exist, whereas if it is below the lower bound of this range, only dark soliton will exist. Chunyang Ma, Bo Gao, Ge Wu, Tian Zhang, and Xiaojian Tian Copyright © 2016 Chunyang Ma et al. All rights reserved. Swimming Exercise Alleviated Insulin Resistance by Regulating Tripartite Motif Family Protein 72 Expression and AKT Signal Pathway in Sprague-Dawley Rats Fed with High-Fat Diet Mon, 24 Oct 2016 06:25:39 +0000 http://www.hindawi.com/journals/jdr/2016/1564386/ We aimed to investigate whether swimming exercise could improve insulin resistance (IR) by regulating tripartite motif family protein 72 (TRIM72) expression and AKT signal pathway in rats fed with high-fat diet. Five-week-old rats were classified into 3 groups: standard diet as control (CON), high-fat diet (HFD), and HFD plus swimming exercise (Ex-HFD). After 8 weeks, glucose infusion rate (GIR), markers of oxidative stress, mRNA and protein expression of TRIM72, protein of IRS, p-AK, and AKT were determined in quadriceps muscles. Compared with HFD, the GIR, muscle SOD, and GSH-Px were significantly increased (, resp.), whereas muscle MDA and 8-OHdG levels were significantly decreased ( and ) in Ex-HFD. Expression levels of TRIM72 mRNA and protein in muscles were significantly reduced ( and ), whereas protein expression levels of IRS-1, p-AK, and AKT were significantly increased in Ex-HFD compared with HFD (, , and ). These results suggest that an 8-week swimming exercise improves HFD-induced insulin resistance maybe through a reduction of TRIM72 in skeletal muscle and enhancement of AKT signal transduction. Jie Qi, Bo Yang, Cailing Ren, Jian Fu, and Jun Zhang Copyright © 2016 Jie Qi et al. All rights reserved. Procaine Induces Epigenetic Changes in HCT116 Colon Cancer Cells Mon, 24 Oct 2016 06:15:55 +0000 http://www.hindawi.com/journals/gri/2016/8348450/ Colon cancer is the third most commonly diagnosed cancer in the world, and it is the major cause of morbidity and mortality throughout the world. The present study aimed at treating colon cancer cell line (HCT116) with different chemotherapeutic drug/drug combinations (procaine, vorinostat “SAHA,” sodium phenylbutyrate, erlotinib, and carboplatin). Two different final concentrations were applied: 3 μM and 5 μM. Trypan blue test was performed to assess the viability of the cell before and after being treated with the drugs. The data obtained showed that there was a significant decrease in the viability of cells after applying the chemotherapeutic drugs/drug combinations. Also, DNA fragmentation assay was carried out to study the effect of these drugs on the activation of apoptosis-mediated DNA degradation process. The results indicated that all the drugs/drug combinations had a severe effect on inducing DNA fragmentation. Global DNA methylation quantification was performed to identify the role of these drugs individually or in combination in hypo- or hypermethylating the CpG dinucleotide all over the genome of the HCT116 colon cancer cell line. Data obtained indicated that different combinations had different effects in reducing or increasing the level of methylation, which might indicate the effectiveness of combining drugs in treating colon cancer cells. Hussein Sabit, Mariam B. Samy, Osama A. M. Said, and Mokhtar M. El-Zawahri Copyright © 2016 Hussein Sabit et al. All rights reserved. Neck of Femur Fracture in a Patient with a Chronic Osteomyelitis of the Ipsilateral Foot Mon, 24 Oct 2016 06:13:21 +0000 http://www.hindawi.com/journals/crior/2016/2108537/ This case report describes a successful two-stage treatment in a 75-year-old male with a displaced neck of femur fracture, also suffering from an active chronic osteomyelitis of the ipsilateral calcaneus. In our case, a below-knee amputation was performed first, followed by total hip arthroplasty two weeks later. At 15-month follow-up, full recovery of the prefracture level of activities of daily living without significant impairment was obtained. Only a few cases of total hip arthroplasty in amputees have been published, but the indication for surgery was mainly traumatic or advanced osteoarthritis. Treating patients with this type of comorbidities is challenging; therapeutic dilemmas can be major. The management in cases like these requires a thorough evaluation and a clear surgical and medical treatment plan, preferably conducted by a multidisciplinary orthogeriatric team. Anne-Carolin Döring, Anne J. H. Vochteloo, Kees van Doorn, Rianne M. H. A. Huis in ’t Veld, and Anil Peters Copyright © 2016 Anne-Carolin Döring et al. All rights reserved. miR-155 Regulated Inflammation Response by the SOCS1-STAT3-PDCD4 Axis in Atherogenesis Mon, 24 Oct 2016 06:03:07 +0000 http://www.hindawi.com/journals/mi/2016/8060182/ Inflammation response plays a critical role in all phases of atherosclerosis (AS). Increased evidence has demonstrated that miR-155 mediates inflammatory mediators in macrophages to promote plaque formation and rupture. However, the precise mechanism of miR-155 remains unclear in AS. Here, we also found that miR-155 and PDCD4 were elevated in the aortic tissue of atherosclerotic mice and ox-LDL treated RAW264.7 cells. Further studies showed that miR-155 not only directly inhibited SOCS1 expression, but also increased the expression of p-STAT and PDCD4, as well as the production of proinflammation mediators IL-6 and TNF-α. Downregulation of miR-155 and PDCD4 and upregulation of SOCS1 obviously decreased the IL-6 and TNF-α expression. In addition, inhibition of miR-155 levels in atherosclerotic mice could notably reduce the IL-6 and TNF-α level in plasma and aortic tissue, accompanied with increased p-STAT3 and PDCD4 and decreased SOCS1. Thus, miR-155 might mediate the inflammation in AS via the SOCS1-STAT3-PDCD4 axis. These results provide a rationale for intervention of intracellular miR-155 as possible antiatherosclerotic targets. Jinshan Ye, Ruiwei Guo, Yankun Shi, Feng Qi, Chuanming Guo, and Lixia Yang Copyright © 2016 Jinshan Ye et al. All rights reserved. A Huge Cystic Retroperitoneal Lymphangioma Presenting with Back Pain Sun, 23 Oct 2016 14:29:46 +0000 http://www.hindawi.com/journals/crim/2016/1618393/ Retroperitoneal lymphangioma is a rare location and type of benign abdominal tumors. The clinical presentation of this rare disease is nonspecific, ranging from abdominal distention to sepsis. Here we present a 73-year-old female patient with 3-month history of back pain. USG and CT revealed a huge cystic mass which was surgically excised and appeared to be lymphangioma on histopathology. Mahir Gachabayov, Kubach Kubachev, Elbrus Abdullaev, Valentin Babyshin, Dmitriy Neronov, and Abakar Abdullaev Copyright © 2016 Mahir Gachabayov et al. All rights reserved. Expression of BMP and Actin Membrane Bound Inhibitor Is Increased during Terminal Differentiation of MSCs Sun, 23 Oct 2016 14:28:30 +0000 http://www.hindawi.com/journals/sci/2016/2685147/ Chondrogenic differentiating mesenchymal stem cells (MSCs) are mimicking embryonal endochondral ossification and become hypertrophic. BMP (bone morphogenetic protein) and Activin Membrane Bound Inhibitor (BAMBI) is a pseudoreceptor that regulates the activity of transforming growth factor-β (TGF-β) and BMP signalling during chondrogenesis. Both TGF-β and BMP signalling are regulators of chondrogenic cell differentiation. Human bone marrow derived MSCs were chondrogenically predifferentiated in aggregate culture for 14 days. Thereafter, one group was subjected to hypertrophy enhancing media conditions while controls were kept in chondrogenic medium until day 28. Histological evaluation, gene expression by PCR, and Western blot analysis were carried out at days 1, 3, 7, 14, 17, 21, and 28. A subset of cultures was treated with the BMP inhibitor Noggin to test for BMP dependent expression of BAMBI. Hypertrophic differentiated pellets showed larger cells with increased collagen 10 and alkaline phosphatase staining. There was significantly increased expression of BAMBI on gene expression and protein level in hypertrophic cultures compared to the chondrogenic control and increased BMP4 gene expression. Immunohistochemistry showed intense staining of BAMBI in hypertrophic cells. BAMBI expression was dose-dependently downregulated by Noggin. The pseudoreceptor BAMBI is upregulated upon enhancement of hypertrophy in MSC chondrogenic differentiation by a BMP dependent mechanism. Christian G. Pfeifer, Alexandra Karl, Arne Berner, Johannes Zellner, Paul Schmitz, Markus Loibl, Matthias Koch, Peter Angele, Michael Nerlich, and Michael B. Mueller Copyright © 2016 Christian G. Pfeifer et al. All rights reserved. Path Planning and Replanning for Mobile Robot Navigation on 3D Terrain: An Approach Based on Geodesic Sun, 23 Oct 2016 14:24:57 +0000 http://www.hindawi.com/journals/mpe/2016/2539761/ In this paper, mobile robot navigation on a 3D terrain with a single obstacle is addressed. The terrain is modelled as a smooth, complete manifold with well-defined tangent planes and the hazardous region is modelled as an enclosing circle with a hazard grade tuned radius representing the obstacle projected onto the terrain to allow efficient path-obstacle intersection checking. To resolve the intersections along the initial geodesic, by resorting to the geodesic ideas from differential geometry on surfaces and manifolds, we present a geodesic-based planning and replanning algorithm as a new method for obstacle avoidance on a 3D terrain without using boundary following on the obstacle surface. The replanning algorithm generates two new paths, each a composition of two geodesics, connected via critical points whose locations are found to be heavily relying on the exploration of the terrain via directional scanning on the tangent plane at the first intersection point of the initial geodesic with the circle. An advantage of this geodesic path replanning procedure is that traversability of terrain on which the detour path traverses could be explored based on the local Gauss-Bonnet Theorem of the geodesic triangle at the planning stage. A simulation demonstrates the practicality of the analytical geodesic replanning procedure for navigating a constant speed point robot on a 3D hill-like terrain. Kun-Lin Wu, Ting-Jui Ho, Sean A. Huang, Kuo-Hui Lin, Yueh-Chen Lin, and Jing-Sin Liu Copyright © 2016 Kun-Lin Wu et al. All rights reserved. Parental Perceptions and Practices toward Childhood Asthma Sun, 23 Oct 2016 14:16:17 +0000 http://www.hindawi.com/journals/bmri/2016/6364194/ Introduction. Parental perceptions and practices are important for improving the asthma outcomes in children; indeed, evidence shows that parents of asthmatic children harbor considerable misperceptions of the disease. Objective. To investigate the perceptions and practices of parents toward asthma and its management in Saudi children. Methods. Using a self-administered questionnaire, a two-stage cross-sectional survey of parents of children aged between 3 and 15 years, was conducted from schools located in Riyadh province in central Saudi Arabia. Results. During the study interval, 2000 parents were asked to participate in the study; 1450 parents responded, of whom 600 (41.4%) reported that their children had asthma, dyspnea, or chest allergy (recurrent wheezing or coughing), while 478 (32.9%) of the parents reported that their children were diagnosed earlier with asthma by a physician. Therefore, the final statistical analyses were performed with 600 participants. Furthermore, 321 (53.5%) respondents believed that asthma is solely a hereditary disease. Interestingly, 361 (60.3%) were concerned about side effects of inhaled corticosteroids and 192 (32%) about the development of dependency on asthma medications. Almost 76% of parents had previously visited a pediatric emergency department during an asthma attack. Conclusions. Parents had misperceptions regarding asthma and exhibited ineffective practices in its management. Therefore, improving asthma care and compliance requires added parental education. Amani K. Abu-Shaheen, Abdullah Nofal, and Humariya Heena Copyright © 2016 Amani K. Abu-Shaheen et al. All rights reserved. Academic Outcomes and Utility of Gastroenterology Residents In-Training Course Abstract Presentations: The University of British Columbia Experience Sun, 23 Oct 2016 14:04:47 +0000 http://www.hindawi.com/journals/cjgh/2016/8698632/ Faisal Al Shatti, Vladimir Marquez Azalagara, and Eric M. Yoshida Copyright © 2016 Faisal Al Shatti et al. All rights reserved. Relationship between the LHPP Gene Polymorphism and Resting-State Brain Activity in Major Depressive Disorder Sun, 23 Oct 2016 13:54:01 +0000 http://www.hindawi.com/journals/np/2016/9162590/ A single-nucleotide polymorphism at the LHPP gene (rs35936514) has been reported in genome-wide association studies to be associated with major depressive disorder (MDD). However, the neural system effects of rs35936514 that mediate the association are unknown. The present work explores whether the LHPP rs35936514 polymorphism moderates brain regional activity in MDD. A total of 160 subjects were studied: a CC group homozygous for the C allele (23 individuals with MDD and 57 controls) and a T-carrier group carrying the high risk T allele (CT/TT genotypes; 22 MDD and 58 controls). All participants underwent resting-state functional magnetic resonance imaging (rs-fMRI) scanning. Brain activity was assessed using the amplitudes of low-frequency fluctuations (ALFF). MDD patients showed a significant increased ALFF in the left middle temporal gyrus and occipital cortex. The T-carrier group showed increased ALFF in the left superior temporal gyrus. Significant diagnosis × genotype interaction was noted in the bilateral lingual gyri, bilateral dorsal lateral prefrontal cortex (dlPFC), and left medial prefrontal cortex (mPFC) (, corrected). Results demonstrated that MDD patients with LHPP rs35936514 CT/TT genotype may influence the regional brain activity. These findings implicate the effects of the rs35936514 variation on the neural system in MDD. Lingling Cui, Xiaohong Gong, Yanqing Tang, Lingtao Kong, Miao Chang, Haiyang Geng, Ke Xu, and Fei Wang Copyright © 2016 Lingling Cui et al. All rights reserved. Wnt-β-Catenin Signaling Promotes the Maturation of Mast Cells Sun, 23 Oct 2016 13:53:48 +0000 http://www.hindawi.com/journals/bmri/2016/2048987/ Mast cells play an important role in the pathogenesis of allergic diseases. Immature mast cells migrate into peripheral tissues from the bone marrow and undergo complete maturation. Interestingly, mast cells have characteristics similar to hematopoietic stem cells (HSCs), such as self-renewal and c-kit expression. In HSCs, Wnt signaling is involved in their maintenance and differentiation. On the other hand, the relation between Wnt signaling and mast cell differentiation is poorly understood. To study whether Wnt signals play a role in the maturation of mast cells, we studied the effect of Wnt proteins on mast cell maturation of bone marrow-derived mast cells (BMMCs). The expression levels of CD81 protein and histidine decarboxylase mRNA and activity of mast cell-specific protease were all elevated in BMMCs treated with Wnt5a. In addition, Wnt5a induced the expression of Axin2 and TCF mRNA in BMMCs. These results showed that Wnt5a could promote the maturation of mast cells via the canonical Wnt signaling pathway and provide important insights into the molecular mechanisms underlying the differentiation of mast cells. Tomoko Yamaguchi, Misae Nishijima, Katsuhisa Tashiro, and Kenji Kawabata Copyright © 2016 Tomoko Yamaguchi et al. All rights reserved. A Design of Wide Band and Wide Beam Cavity-Backed Slot Antenna Array with Slant Polarization Sun, 23 Oct 2016 13:31:15 +0000 http://www.hindawi.com/journals/ijap/2016/8980495/ Design of antenna array under the limitation of restricted size is a challenging problem. Cavity-backed slot antenna is widely used because of its advantages of small size, wide band, and wide beam. In this paper, a design of wide band and wide beam cavity-backed slot antenna array with the slant polarization is proposed. To obtain wide band and wide beam with limited size, the inverted microstrip-fed cavity-backed slot antenna (IMF-CBSA) is adopted as the element of 1 × 4 antenna array. The slant polarized antennas and their feeding networks are adopted because of their simple structures. The performance of the proposed antenna array is verified by the simulations and experiments. The measured VSWR < 2 bandwidth is 55% at the center frequency 21.8 GHz, and the gain is larger than 12.2 dB. Experimental results demonstrate that the proposed design achieves wide band and beam with the size of 68 mm × 56 mm × 14.5 mm. Huiying Qi, Ke Xiao, Fei Zhao, Shunlian Chai, and Wenlu Yin Copyright © 2016 Huiying Qi et al. All rights reserved. Localisation of Lactate Transporters in Rat and Rabbit Placentae Sun, 23 Oct 2016 13:28:09 +0000 http://www.hindawi.com/journals/ijcb/2016/2084252/ The distribution of monocarboxylate transporter (MCT) isoforms 1 and 4, which mediate the plasmalemmal transport of L-lactic and pyruvic acids, has been identified in the placentae of rats and rabbits at different ages of gestation. Groups of three pregnant Sprague-Dawley rats and New Zealand White rabbits were sacrificed on gestation days (GD) 11, 14, 18, or 20 and on GD 13, 18, or 28, respectively. Placentae were removed and processed for immunohistochemical detection of MCT1 and MCT4. In the rat, staining for MCT1 was associated with lakes and blood vessels containing enucleated red blood cells (maternal vessels) while staining for MCT4 was associated with vessels containing nucleated red blood cells (embryofoetal vessels). In the rabbit, staining for MCT1 was associated with blood vessels containing nucleated red blood cells while staining for MCT4 was associated with vessels containing enucleated red blood cells. Strength of staining for MCT1 decreased during gestation in both species, but that for MCT4 was stronger than that for MCT1 and was consistent between gestation days. The results imply an opposite polarity of MCT1 and MCT4 across the trophoblast between rat and rabbit. Nigel P. Moore, Catherine A. Picut, and Jeffrey H. Charlap Copyright © 2016 Nigel P. Moore et al. All rights reserved. Reliability and Validity Measurement of Sagittal Lumbosacral Quiet Standing Posture with a Smartphone Application in a Mixed Population of 183 College Students and Personnel Sun, 23 Oct 2016 13:21:42 +0000 http://www.hindawi.com/journals/aorth/2016/3817270/ Accurate recording of spinal posture with simple and accessible measurement devices in clinical practice may lead to spinal loading optimization in occupations related to prolonged sitting and standing postures. Therefore, the purpose of this study was to establish the level of reliability of sagittal lumbosacral posture in quiet standing and the validity of the method in differentiating between male and female subjects, establishing in parallel a normative database. 183 participants (83 males and 100 females), with no current low back or pelvic pain, were assessed using the “iHandy Level” smartphone application. Intrarater reliability (3 same-day sequential measurements) was high for both the lumbar curve (: 0.96, SEM: 2.13°, and : 5.9°) and the sacral slope (: 0.97, SEM: 1.61°, and : 4.46°) sagittal alignment. Data analysis for each gender separately confirmed equally high reliability for both male and female participants. Correlation between lumbar curve and sacral slope was high (Pearson’s , ). Between-gender comparisons confirmed the validity of the method to differentiate between male and female lumbar curve and sacral slope angles, with females generally demonstrating greater lumbosacral values (). The “iHandy Level” application is a reliable and valid tool in the measurement of lumbosacral quiet standing spinal posture in the sagittal plane. George A. Koumantakis, Maria Nikoloudaki, Sara Thacheth, Kalliroi Zagli, Konstantina Bitrou, Andreas Nigritinos, and Leon Botton Copyright © 2016 George A. Koumantakis et al. All rights reserved. Considerations for Defining Cytokine Dose, Duration, and Milieu That Are Appropriate for Modeling Chronic Low-Grade Inflammation in Type 2 Diabetes Sun, 23 Oct 2016 13:16:04 +0000 http://www.hindawi.com/journals/jdr/2016/2846570/ Proinflammatory cytokines have been implicated in the pathophysiology of both type 1 diabetes (T1D) and type 2 diabetes (T2D). T1D is an autoimmune disease involving the adaptive immune system responding to pancreatic beta-cells as antigen-presenting cells. This attracts immune cells that surround pancreatic islets (insulitis) and secrete cytokines, such as IL-1beta, IFN-gamma, and TNF-alpha, in close proximity to pancreatic beta-cells. In contrast, there is little evidence for such a focused autoimmune response in T2D. Instead, the innate immune system, which responds to cellular damage and pathogens, appears to play a key role. There are three major sources of proinflammatory cytokines that may impact islet/beta-cell function in T2D: (1) from islet cells, (2) from increased numbers of intraislet macrophages/immune cells, and (3) from increased circulating levels of proinflammatory cytokines due to obesity, presumably coming from inflamed adipose tissue. These differences between T1D and T2D are reflected by significant differences in the cytokine concentration, duration, and milieu. This review focuses on chronic versus acute cytokine action, cytokine concentrations, and cytokine milieu from the perspective of the pancreatic islet in T2D. We conclude that new cytokine models may be needed to reflect the pathophysiology of T2D more effectively than what are currently employed. Craig S. Nunemaker Copyright © 2016 Craig S. Nunemaker. All rights reserved. Protective Effect of Psoralea corylifolia L. Seed Extract against Palmitate-Induced Neuronal Apoptosis in PC12 Cells Sun, 23 Oct 2016 13:12:10 +0000 http://www.hindawi.com/journals/ecam/2016/5410419/ The extract of Psoralea corylifolia seeds (PCE) has been widely used as a herbal medicine because of its beneficial effect on human health. In this study, we investigated the protective effects and molecular mechanisms of PCE on palmitate- (PA-) induced toxicity in PC12 cells, a neuron-like cell line. PCE significantly increased cell viability in PA-treated PC12 cells and showed antiapoptotic effects, as evidenced by decreased expression of cleaved caspase-3, cleaved poly(ADP-ribose) polymerase, and bax protein as well as increased expression of bcl-2 protein. In addition, PCE treatment reduced PA-induced reactive oxygen species production and upregulated mRNA levels of antioxidant genes such as nuclear factor (erythroid-derived 2)-like 2 and heme oxygenase 1. Moreover, PCE treatment recovered the expression of autophagy marker genes such as beclin-1 and p62, which was decreased by PA treatment. Treatment with isopsoralen, one of the major components of PCE extract, also recovered the expression of autophagy marker genes and reduced PA-induced apoptosis. In conclusion, PCE exerts protective effects against lipotoxicity via its antioxidant function, and this effect is mediated by activation of autophagy. PCE might be a potential pharmacological agent to protect against neuronal cell injury caused by oxidative stress or lipotoxicity. Yunkyoung Lee, Hee-Sook Jun, and Yoon Sin Oh Copyright © 2016 Yunkyoung Lee et al. All rights reserved. Implant-Retained Obturator for an Edentulous Patient with a Hemimaxillectomy Defect Complicated with Microstomia Sun, 23 Oct 2016 13:08:59 +0000 http://www.hindawi.com/journals/crid/2016/4618510/ Patient. A 68-year-old man was operated on for squamous cell carcinoma (T3N3M0) of the maxilla creating the hemimaxillary surgical defect on right side. The remaining arch was completely edentulous. There was remarkable limitation in the oral opening with reduced perimeter of the oral cavity due to radiation and surgical scar contracture. This article describes prosthetic rehabilitation by modifying the design of the obturator and achieving the retention with dental implant. Discussion. Severe limitation in the oral opening may occur in clinical situations following the postsurgical management of oral and maxillofacial defects. The prosthetic rehabilitation of the surgical defect in such patients becomes a challenging task due to limited access to the oral cavity. This challenge becomes even more difficult if the patient is edentulous and there are no teeth to gain the retention, stability, and support. Conclusion. In severe microstomia prosthesis insertion and removal can be achieved with modification of the maximum width of the prosthesis. Dental implant retention is useful treatment option in edentulous patients with maxillary surgical defect provided that sufficient bone volume and accessibility are there for implant placement. Pravinkumar G. Patil and Smita Nimbalkar-Patil Copyright © 2016 Pravinkumar G. Patil and Smita Nimbalkar-Patil. All rights reserved. Recent Progress on Nanostructures for Drug Delivery Applications Sun, 23 Oct 2016 13:05:52 +0000 http://www.hindawi.com/journals/jnm/2016/5762431/ With the rapid development of nanotechnology, the convergence of nanostructures and drug delivery has become a research hotspot in recent years. Due to their unique and superior properties, various nanostructures, especially those fabricated from self-assembly, are able to significantly increase the solubility of poorly soluble drugs, reduce cytotoxicity toward normal tissues, and improve therapeutic efficacy. Nanostructures have been successfully applied in the delivery of diverse drugs, such as small molecules, peptides, proteins, and nucleic acids. In this paper, the driving forces for the self-assembly of nanostructures are introduced. The strategies of drug delivery by nanostructures are briefly discussed. Furthermore, the emphasis is put on a variety of nanostructures fabricated from various building materials, mainly liposomes, polymers, ceramics, metal, peptides, nucleic acids, and even drugs themselves. Haijiao Lu, Jingkang Wang, Ting Wang, Jian Zhong, Ying Bao, and Hongxun Hao Copyright © 2016 Haijiao Lu et al. All rights reserved. Elder Orphans Hiding in Plain Sight: A Growing Vulnerable Population Sun, 23 Oct 2016 13:05:38 +0000 http://www.hindawi.com/journals/cggr/2016/4723250/ Adults are increasingly aging alone with multiple chronic diseases and are geographically distant from family or friends. It is challenging for clinicians to identify these individuals, often struggling with managing the growing difficulties and the complexities involved in delivering care to this population. Clinicians often may not recognize or know how to address the needs that these patients have in managing their own health. While many such patients function well at baseline, the slightest insult can initiate a cascade of avoidable negative events. We have resurrected the term elder orphan to describe individuals living alone with little to no support system. Using public data sets, including the US Census and University of Michigan’s Health and Retirement Study, we estimated the prevalence of adults 65 years and older to be around 22%. Thus, in this paper, we strive to describe and quantify this growing vulnerable population and offer practical approaches to identify and develop care plans that are consistent with each person’s goals of care. The complex medical and psychosocial issues for elder orphans significantly impact the individual person, communities, and health-care expenditures. We hope to encourage professionals across disciplines to work cooperatively to screen elders and implement policies to prevent elder orphans from hiding in plain sight. Maria T. Carney, Janice Fujiwara, Brian E. Emmert Jr., Tara A. Liberman, and Barbara Paris Copyright © 2016 Maria T. Carney et al. All rights reserved. Multidimensional Schrödinger Equation and Spectral Properties of Heavy-Quarkonium Mesons at Finite Temperature Sun, 23 Oct 2016 12:50:10 +0000 http://www.hindawi.com/journals/amp/2016/4935940/ The -radial Schrödinger equation is analytically solved at finite temperature. The analytic exact iteration method (AEIM) is employed to obtain the energy eigenvalues and wave functions for all states and . The application of present results to the calculation of charmonium and bottomonium masses at finite temperature is also presented. The behavior of the charmonium and bottomonium masses is in qualitative agreement with other theoretical methods. We conclude that the solution of the Schrödinger equation plays an important role at finite temperature that the analysis of the quarkonium states gives a key input to quark-gluon plasma diagnostics. M. Abu-Shady Copyright © 2016 M. Abu-Shady. All rights reserved. Joint Radio Resource Allocation and Base Station Location Selection in OFDMA Based Private Wireless Access Networks for Smart Grid Sun, 23 Oct 2016 12:33:24 +0000 http://www.hindawi.com/journals/jece/2016/7948018/ This paper studies the base stations deployment problem in orthogonal frequency-division multiple access (OFDMA) based private wireless access networks for smart grid (SG). Firstly, we analyze the differences between private wireless access networks for SG and public cellular access networks. Then, we propose scheduling and power control based algorithms for the radio resource allocation subproblem and K-means, simulated annealing (SA), and particle swarm optimization (PSO) based algorithms for the base station (BS) location selection subproblem and iterate over these two sets of algorithms to solve the target problem. Simulation results show that the proposed method can effectively solve the target problem. Specifically, the combination of power control based resource allocation algorithm and PSO based location selection algorithm is recommended. Peng Du and Yuan Zhang Copyright © 2016 Peng Du and Yuan Zhang. All rights reserved. The Influence of Methylsulfonylmethane on Inflammation-Associated Cytokine Release before and following Strenuous Exercise Sun, 23 Oct 2016 12:27:01 +0000 http://www.hindawi.com/journals/jsm/2016/7498359/ Background. Inflammation is associated with strenuous exercise and methylsulfonylmethane (MSM) has been shown to have anti-inflammatory properties. Methods. Physically active men were supplemented with either placebo or MSM (3 grams per day) for 28 days before performing 100 repetitions of eccentric knee extension exercise. Ex vivo and in vitro testing consisted of evaluating cytokine production in blood (whole blood and isolated peripheral blood mononuclear cells (PBMCs)) exposed to lipopolysaccharide (LPS), before and through 72 hours after exercise, while in vivo testing included the evaluation of cytokines before and through 72 hours after exercise. Results. LPS stimulation of whole blood after MSM supplementation resulted in decreased induction of IL-1β, with no effect on IL-6, TNF-α, or IL-8. After exercise, there was a reduced response to LPS in the placebo, but MSM resulted in robust release of IL-6 and TNF-α. A small decrease in resting levels of proinflammatory cytokines was noted with MSM, while an acute postexercise increase in IL-10 was observed with MSM. Conclusion. Strenuous exercise causes a robust inflammatory reaction that precludes the cells from efficiently responding to additional stimuli. MSM appears to dampen the release of inflammatory molecules in response to exercise, resulting in a less incendiary environment, allowing cells to still have the capacity to mount an appropriate response to an additional stimulus after exercise. Mariè van der Merwe and Richard J. Bloomer Copyright © 2016 Mariè van der Merwe and Richard J. Bloomer. All rights reserved. On the Interval Stability of Weak-Nonlinear Control Systems with Aftereffect Sun, 23 Oct 2016 12:17:48 +0000 http://www.hindawi.com/journals/tswj/2016/6490826/ Sufficient conditions of interval absolute stability of nonlinear control systems described in terms of systems of the ordinary differential equations with delay argument and also neutral type are obtained. The Lyapunov-Krasovskii functional method in the form of the sum of a quadratic component and integrals from nonlinearity is used at construction of statements. Andriy Shatyrko and Denys Khusainov Copyright © 2016 Andriy Shatyrko and Denys Khusainov. All rights reserved. The Repulsive Casimir Force with Metallic Ellipsoid Structure Sun, 23 Oct 2016 12:14:40 +0000 http://www.hindawi.com/journals/jnt/2016/1746908/ We propose a new structure, one plate with a hole above the ellipsoid and the other plate with a hole below the ellipsoid, to obtain a repulsive Casimir force. The force was obtained numerically by using the in-house FDTD method, based on Maxwell’s stress tensor and harmonic expansion. The code can be verified by calculating the force of a perfect-metal ellipsoid centered above a perfect-metal plate with a hole. Our numerical method can effectively simulate the Casimir force by reducing the total simulated time. The further numerical results of realistic dielectric material immersing in fluids or adding other plates above the ellipsoid are also presented. It is not surprising to find that the larger differences can be achieved by varying the parameters such as the center-center separation, medium immersed, and the dielectric material of the structure. Thus, it is possible to tune these parameters relatively in the realistic microelectromechanical systems to overcome stiction and friction problems. Yujuan Hu, Ruo Sun, Zhixiang Huang, and Xianliang Wu Copyright © 2016 Yujuan Hu et al. All rights reserved. Synergism Analysis of Bedding Slope with Piles and Anchor Cable Support under Sine Wave Vehicle Load Sun, 23 Oct 2016 12:11:44 +0000 http://www.hindawi.com/journals/amse/2016/5753970/ Slope instability under dynamic load is the technical difficulty in the engineering; the evaluation of slope stability under dynamic load and the control of dynamic load is particularly important. In this paper, taking the right side slope of K27+140 m~380 m typical section (K27 slope for short) in Chongqing Fuling-Fengdu-Shizhu expresses highway as an example to calculate and analyze. The K27 slope is under sinusoidal vehicle load and supported by anchor cable and antislide pile to resist downslide strength; at the same time, the combined effect of them is studied. Three-dimensional finite element methodology (FEM) is used to simulate the bedding slope with piles and anchor cable support; furthermore, the eigenvalue can be obtained. In order to reduce error of the elastic boundary conditions caused by the reflection effect of wavelengths, the combination of Lysmer surface viscous boundary and traditional ground support boundaries is utilized to analyze and calculate the time-histories during bedding slope under dynamic load. The dynamic response of pile anchor support to resist sliding force is obtained. The concept of the pile anchor supporting coordinate interval is put forward. Furthermore, it is verified that the pile anchor supporting coordinate interval can be used to evaluate the stability of the slope under dynamic load and provide a new method for the control of the dynamic load. Li Dan-Feng and Wang Lian-Jun Copyright © 2016 Li Dan-Feng and Wang Lian-Jun. All rights reserved. Effects of Inositol(s) in Women with PCOS: A Systematic Review of Randomized Controlled Trials Sun, 23 Oct 2016 12:05:49 +0000 http://www.hindawi.com/journals/ije/2016/1849162/ Polycystic ovary syndrome (PCOS) is a common endocrine disorder, with complex etiology and pathophysiology, which remains poorly understood. It affects about 5–10% of women of reproductive age who typically suffer from obesity, hyperandrogenism, ovarian dysfunction, and menstrual irregularity. Indeed, PCOS is the most common cause of anovulatory infertility in industrialized nations, and it is associated with insulin resistance, type 2 diabetes mellitus, and increased cardiovascular risk. Although insulin resistance is not included as a criterion for diagnosis, it is a critical pathological condition of PCOS. The purpose of this systematic review is the analysis of recent randomized clinical trials of inositol(s) in PCOS, in particular myo- and D-chiro-inositol, in order to better elucidate their physiological involvement in PCOS and potential therapeutic use, alone and in conjunction with assisted reproductive technologies, in the clinical treatment of women with PCOS. Vittorio Unfer, John E. Nestler, Zdravko A. Kamenov, Nikos Prapas, and Fabio Facchinetti Copyright © 2016 Vittorio Unfer et al. All rights reserved. Learning Latent Variable Gaussian Graphical Model for Biomolecular Network with Low Sample Complexity Sun, 23 Oct 2016 11:31:10 +0000 http://www.hindawi.com/journals/cmmm/2016/2078214/ Learning a Gaussian graphical model with latent variables is ill posed when there is insufficient sample complexity, thus having to be appropriately regularized. A common choice is convex plus nuclear norm to regularize the searching process. However, the best estimator performance is not always achieved with these additive convex regularizations, especially when the sample complexity is low. In this paper, we consider a concave additive regularization which does not require the strong irrepresentable condition. We use concave regularization to correct the intrinsic estimation biases from Lasso and nuclear penalty as well. We establish the proximity operators for our concave regularizations, respectively, which induces sparsity and low rankness. In addition, we extend our method to also allow the decomposition of fused structure-sparsity plus low rankness, providing a powerful tool for models with temporal information. Specifically, we develop a nontrivial modified alternating direction method of multipliers with at least local convergence. Finally, we use both synthetic and real data to validate the excellence of our method. In the application of reconstructing two-stage cancer networks, “the Warburg effect” can be revealed directly. Yanbo Wang, Quan Liu, and Bo Yuan Copyright © 2016 Yanbo Wang et al. All rights reserved. Continuous Quadrupole Magnetic Separation of Islets during Digestion Improves Purified Porcine Islet Viability Sun, 23 Oct 2016 11:21:34 +0000 http://www.hindawi.com/journals/jdr/2016/6162970/ Islet transplantation (ITx) is an emerging and promising therapy for patients with uncontrolled type 1 diabetes. The islet isolation and purification processes require exposure to extended cold ischemia, warm-enzymatic digestion, mechanical agitation, and use of damaging chemicals for density gradient separation (DG), all of which reduce viable islet yield. In this paper, we describe initial proof-of-concept studies exploring quadrupole magnetic separation (QMS) of islets as an alternative to DG to reduce exposure to these harsh conditions. Three porcine pancreata were split into two parts, the splenic lobe (SPL) and the combined connecting/duodenal lobes (CDL), for paired digestions and purifications. Islets in the SPL were preferentially labeled using magnetic microparticles (MMPs) that lodge within the islet microvasculature when infused into the pancreas and were continuously separated from the exocrine tissue by QMS during the collection phase of the digestion process. Unlabeled islets from the CDL were purified by conventional DG. Islets purified by QMS exhibited significantly improved viability (measured by oxygen consumption rate per DNA, ) and better morphology relative to control islets. Islet purification by QMS can reduce the detrimental effects of prolonged exposure to toxic enzymes and density gradient solutions and substantially improve islet viability after isolation. Bradley P. Weegman, Venkata Sunil Kumar Sajja, Thomas M. Suszynski, Michael D. Rizzari, William E. Scott III, Jennifer P. Kitzmann, Kate R. Mueller, Thomas R. Hanley, David J. Kennedy, Paul W. Todd, Appakalai N. Balamurugan, Bernhard J. Hering, and Klearchos K. Papas Copyright © 2016 Bradley P. Weegman et al. All rights reserved. Prevalence and Correlates of Complementary and Alternative Medicine Use among Hypertensive Patients in Gondar Town, Ethiopia Sun, 23 Oct 2016 11:18:06 +0000 http://www.hindawi.com/journals/ecam/2016/6987636/ Background. Complementary and alternative medicine (CAM) therapies are being widely used by hypertensive patients worldwide. However, evidences regarding CAM use by hypertensive patients in Ethiopia are limited. This study aimed at assessing prevalence and correlates of CAM use among hypertensive patients attending ambulatory clinic at Gondar University Referral Hospital (GURH), Ethiopia. Methods. A cross-sectional study was employed on 423 patients visiting GURH. Descriptive statistics and bivariate and multivariate logistic regression tools were used to analyze/come up with the prevalence and correlates of CAM use. Results. The prevalence of CAM use in our study was found to be 67.8% and herbal based medicine was the most commonly utilized CAM therapies. Majority of CAM users (70.2%) did not disclose CAM use for their physician. However, nearly half of CAM users (48.4%) were satisfied with the result of CAM use. Conclusions. The higher prevalence of CAM use among hypertensive patients coupled with a very low disclosure rate to their health care providers can have a marked potential to cause ineffective hypertensive management and adverse effects due to CAM use. Health care providers should be open to discussing the use of CAM with their patients as it will lead to better health outcome. Daniel Asfaw Erku and Abebe Basazn Mekuria Copyright © 2016 Daniel Asfaw Erku and Abebe Basazn Mekuria. All rights reserved. Could Total Colectomy with Ileorectal Anastomosis Be an Alternative to Total Proctocolectomy with Ileal Pouch-Anal Anastomosis in Selected Ulcerative Colitis Patients? Sun, 23 Oct 2016 11:16:36 +0000 http://www.hindawi.com/journals/grp/2016/5832743/ Purpose. To evaluate ileorectal anastomosis (IRA) in selected ulcerative colitis patients. Methods. Early and late complications after IRA and IPAA were investigated. Bowel function and quality of life were assessed. Functional and QoL studies were performed as a matched pair analysis, comparing 98 patients who underwent IRA versus 98 patients who underwent IPAA. Results. In IRA group, 2 patients (1.6%) developed anastomotic l dysplasia (HGD) developed in 3 patients dysplasia (HGD) developed in 3 patients eakage, 1 patient (0.8%) had intestinal obstruction, and 2 patients (1.6%) had abdominal hematoma. Mean follow-up was 11.5 (range: 2–24.3) years. Failure of IRA occurred in 19 patients (15.1%); in 12 patients (9.5%), failure was related to severe proctitis, in 3 patients (2.4%), it was related to the development of high-grade dysplasia, and in 4 patients (3.2%), it was related to the development of rectal cancer. About functional results, stool consistency [liquid (6.7% of IRA patients versus 29% of IPAA patients; )], daily soiling (0% versus 6%; ), and nocturnal soiling (6% versus 25.5%; ) were statistically different. Only 1% of IRA patients versus 11% of IPAA patients had episodes of perianal inflammation (). CGQoL was 0.72 (, SD) in IRA patients and 0.75 (, SD) in IPAA patients (). Conclusion. In selected patients, IRA is an appropriate surgical option, with low morbidity, comparable quality of life, and better functional results than IPAA. Francesco Tonelli, Carmela Di Martino, and Francesco Giudici Copyright © 2016 Francesco Tonelli et al. All rights reserved. Tumor Budding, Micropapillary Pattern, and Polyploidy Giant Cancer Cells in Colorectal Cancer: Current Status and Future Prospects Sun, 23 Oct 2016 11:11:55 +0000 http://www.hindawi.com/journals/sci/2016/4810734/ We previously reported that polyploid giant cancer cells (PGCGs) induced by CoCl2 could form through endoreduplication or cell fusion. A single PGCC formed tumors in immunodeficient mice. PGCCs are also the key contributors to the cellular atypia and associate with the malignant grade of tumors. PGCCs have the properties of cancer stem cells and produce daughter cells via asymmetric cell division. Compared with diploid cancer cells, these daughter cells express less epithelial markers and acquire mesenchymal phenotype with importance in cancer development and progression. Tumor budding is generally recognized to correlate with a high recurrence rate, lymph node metastasis, chemoresistance, and poor prognosis of colorectal cancers (CRCs) and is a good indicator to predict the metastasis and aggressiveness in CRCs. Micropapillary pattern is a special morphologic pattern and also associates with tumor metastasis and poor prognosis. There are similar morphologic features and molecular phenotypes among tumor budding, micropapillary carcinoma pattern, and PGCCs with their budding daughter cells and all of them show strong ability of tumor invasion and migration. In this review, we discuss the cancer stem cell properties of PGCCs, the molecular mechanisms of their regulation, and the relationships with tumor budding and micropapillary pattern in CRCs. Shiwu Zhang, Dan Zhang, Zhengduo Yang, and Xipeng Zhang Copyright © 2016 Shiwu Zhang et al. All rights reserved. Challenges in Modelling Hypoglycaemia-Associated Autonomic Failure: A Review of Human and Animal Studies Sun, 23 Oct 2016 11:09:10 +0000 http://www.hindawi.com/journals/ije/2016/9801640/ Recurrent insulin-induced hypoglycaemia is a major limitation to insulin treatment in diabetes patients leading to a condition called hypoglycaemia-associated autonomic failure (HAAF). HAAF is characterised by reduced sympathoadrenal response to subsequent hypoglycaemia thereby predisposing the patients to severe hypoglycaemia that can lead to coma or even death. Despite several attempts being made, the mechanism of HAAF is yet to be clearly established. In order for the mechanism of HAAF to be elucidated, establishing a human/animal model of the phenomenon is the foremost requirement. Several research groups have attempted to reproduce the phenomenon in diabetic and nondiabetic humans and rodents and reported variable results. The success of the phenomenon is marked by a significant reduction in plasma adrenaline response to subsequent hypoglycaemic episode relative to that of the antecedent hypoglycaemic episode. A number of factors such as the insulin dosage, route of administration, fasting conditions, blood sampling methods and analyses, depth, duration, and number of antecedent hypoglycaemic episodes can impact the successful reproduction of the phenomenon and thus have to be carefully considered while developing the protocol. In this review, we have outlined the protocols followed by different research groups to reproduce the phenomenon in diabetic and nondiabetic humans and rodents including our own observations in rats and discussed the factors that have to be given careful consideration in reproducing the phenomenon successfully. Manjula Senthilkumaran, Xin-Fu Zhou, and Larisa Bobrovskaya Copyright © 2016 Manjula Senthilkumaran et al. All rights reserved. Temperature Regulation of Photovoltaic Module Using Phase Change Material: A Numerical Analysis and Experimental Investigation Sun, 23 Oct 2016 10:22:54 +0000 http://www.hindawi.com/journals/ijp/2016/5917028/ This work represents an effective design of a temperature regulated PV module by integrating phase change materials for Malaysian weather condition. Through the numerical analysis and experimental investigation it has been shown that if a PCM layer of width 0.02 m of RT 35 is used as a cooling arrangement with a PV module, the surface temperature of the module is reduced by 10°C, which remains constant for a period of 4–6 hours. This reduction of temperature implies the increase in conversion efficiency of the module. Experiment as well as investigation has been carried out considering typical Malaysian weather. Obtained result has been validated by using experimental prototype and comparative analysis. Hasan Mahamudul, Md. Momtazur Rahman, H. S. C. Metselaar, Saad Mekhilef, S. A. Shezan, Rana Sohel, Sayuti Bin Abu Karim, and Wan Nur Izzati Badiuzaman Copyright © 2016 Hasan Mahamudul et al. All rights reserved. A Review of the Topologies Used in Smart Water Meter Networks: A Wireless Sensor Network Application Sun, 23 Oct 2016 10:08:01 +0000 http://www.hindawi.com/journals/js/2016/9857568/ This paper presents several proposed and existing smart utility meter systems as well as their communication networks to identify the challenges of creating scalable smart water meter networks. Network simulations are performed on 3 network topologies (star, tree, and mesh) to determine their suitability for smart water meter networks. The simulations found that once a number of nodes threshold is exceeded the network’s delay increases dramatically regardless of implemented topology. This threshold is at a relatively low number of nodes (50) and the use of network topologies such as tree or mesh helps alleviate this problem and results in lower network delays. Further simulations found that the successful transmission of application layer packets in a 70-end node tree network can be improved by 212% when end nodes only transmit data to their nearest router node. The relationship between packet success rate and different packet sizes was also investigated and reducing the packet size with a factor of 16 resulted in either 156% or 300% increases in the amount of successfully received packets depending on the network setup. Jaco Marais, Reza Malekian, Ning Ye, and Ruchuan Wang Copyright © 2016 Jaco Marais et al. All rights reserved. A Child with Severe Malaria Presenting with Acute Surgical Abdomen (Duodenal Perforation) Sun, 23 Oct 2016 09:57:26 +0000 http://www.hindawi.com/journals/cripe/2016/3092130/ Plasmodium falciparum, the commonest cause of severe malaria in children, is an important cause of mortality in developing nations like Nepal. Duodenal perforation in a case of complicated malaria, although a rare entity, can occur in children. Early diagnosis, proper medical treatment, and early surgical repair can be a lifesaving measure in such cases. Here, we report a case of a 5-year-old male child with falciparum malaria complicated by a duodenal perforation that was successively managed with appropriate antimalarial drugs and early surgical repair. Tika Ram Bhandari, Sudha Shahi, Rajesh Poudel, and Nagendra Chaudhary Copyright © 2016 Tika Ram Bhandari et al. All rights reserved. The NPs Role of Assessing and Intervening with Older Adult Drivers Sun, 23 Oct 2016 09:54:06 +0000 http://www.hindawi.com/journals/nrp/2016/3254857/ As the silver tsunami continues, assessing and intervening with older adult drivers are becoming an essential aspect of the comprehensive geriatric exam. The current lack of time efficient clinical guidelines is a concern and barrier for NPs. The purpose of this study was to identify strategies currently used by NPs. The critical incident technique was used to obtain data from a convenience sample of NPs. A total of 89 incidents were collected. The perspective of the NP can provide important information for developing clinical guidelines to promote individual and community safety. Tamatha Arms Copyright © 2016 Tamatha Arms. All rights reserved. Reactivation of Pulmonary Tuberculosis following Treatment of Myelofibrosis with Ruxolitinib Sun, 23 Oct 2016 09:45:41 +0000 http://www.hindawi.com/journals/crihem/2016/2389038/ Ruxolitinib is widely in use for treatment of myeloproliferative disorders. It causes inhibition of the Janus kinase (JAK) signal transducer and activation of transcription (STAT) pathway, which plays a key role in the underlying pathophysiology of myeloproliferative diseases. We describe a case of reactivation pulmonary tuberculosis in a retired physician while on treatment with ruxolitinib. We also review the literature on opportunistic infections following use of ruxolitinib. Our case highlights the importance of screening for latent tuberculosis in patients from highly endemic areas prior to start of therapy with ruxolitinib. Maheen Z. Abidi, Javeria Haque, Parvathi Varma, Horatiu Olteanu, Guru Subramanian Guru Murthy, Binod Dhakal, and Parameswaran Hari Copyright © 2016 Maheen Z. Abidi et al. All rights reserved. Study on the AFM Force Curve Common Errors and Their Effects on the Calculated Nanomechanical Properties of Materials Sun, 23 Oct 2016 09:35:49 +0000 http://www.hindawi.com/journals/je/2016/2456378/ The atomic force microscope (AFM) force curve has been widely used for determining the mechanical properties of materials due to its high resolution, whereby very low (piconewton) forces and distances as small as nanometers can be measured. However, sometimes the resultant force curve obtained from AFM is slightly different from those obtained from a more typical nanoindentation force curve due to the AFM piezo’s hysteresis. In this study the nanomechanical properties of either a sulfonated polyether ether ketone (SPEEK) treated layer or bare polyether ether ketone (PEEK) were evaluated via AFM nanoindentation and a nanomechanical test system to probe the possible error of the calculated nanomechanical properties due to the AFM piezo’s hysteresis. The results showed that AFM piezo’s hysteresis caused the error in the calculated nanomechanical properties of the materials. D. Almasi, R. Sharifi, M. R. Abdul Kadir, G. Krishnamurithy, and T. Kamarul Copyright © 2016 D. Almasi et al. All rights reserved. Granulovacuolar Degeneration in Hippocampus of Neurodegenerative Diseases: Quantitative Study Sun, 23 Oct 2016 09:35:43 +0000 http://www.hindawi.com/journals/jnd/2016/6163186/ Background. Granulovacuolar degeneration (GVD) is one of the pathological features long associated with Alzheimer’s disease (AD) and normal aging. Aim. We investigate the frequency of GVDs in AD, other neurodegenerative diseases, and normal aging, with attempt to determine whether the GVD has preponderance in any particular neurodegenerative disease other than AD. Materials and Methods. A retrospective review of 111 autopsied cases with a variety of neurodegenerative diseases and 70 control cases without pathological evidence of neurodegeneration was evaluated quantitatively. The microscopic examination was applied on coronal sections of hippocampi using Hematoxylin and Eosin (H&E) and Bielschowsky silver impregnation. The mean percentage of neurons with GVDs was calculated through all sectors of Ammon’s horn for each case. Result. We found that neurons with GVD, in cases with or without neurodegenerative diseases, were found predominantly in CA1 and CA2 sectors of hippocampus. The GVD count in AD was significantly increased in CA1 and CA2 compared to other neurodegenerative cases as well as normal aging controls. In AD/LBD there was a significant increase in GVD in CA1 whereas in LBD there was no significant change in GVD. Conclusions. The frequency of GVD in AD is due to the disease process and attributes the increase for AD/LBD to the AD component. Maher Kurdi, Esther Chin, and Lee Cyn Ang Copyright © 2016 Maher Kurdi et al. All rights reserved. Rare Case of Leiomyoma and Adenomyosis in Mayer-Rokitansky-Kuster-Hauser Syndrome Sun, 23 Oct 2016 09:32:08 +0000 http://www.hindawi.com/journals/criog/2016/3725043/ We report a case of adenomyosis which developed from a hypoplastic uterus and leiomyoma in a patient with MRKH syndrome. A 45-year-old Malay female with primary amenorrhoea and primary infertility presented with abdominal mass and abdominal pain. She is phenotypically female, has well developed secondary sexual characteristics, and has normal female external genitalia with shallow vagina dimple. Transabdominal ultrasonography showed a homogenous adnexal mass of 10 × 8 cm, uterus sized 5 × 4 cm, and normal kidneys. A complex mass of right adnexa was demonstrated by CT scan. Exploratory laparotomy showed torsion of right adnexal mass and rudimentary uterus with fibroid but no endometrial tissue and blind end with absent cervix. The normal right ovary and tube were not visualized. The left fallopian tube and ovary were normal. It is also complicated by vaginal agenesis. Removal of right adnexal mass and rudimentary uterus was done with preservation of left ovary. The histologic diagnosis was uterine adenomyosis and leiomyoma arising from the right adnexa, possibly from the broad ligament. P. S. Hoo, A. R. Norhaslinda, and J. N. Shah Reza Copyright © 2016 P. S. Hoo et al. All rights reserved. A Dog Is a Doctor’s Best Friend: The Use of a Service Dog as a Perioperative Assistant Sun, 23 Oct 2016 09:31:41 +0000 http://www.hindawi.com/journals/cripe/2016/9013520/ Service dogs are beneficial in providing assistance to people with multiple types of disabilities and medical disorders including visual impairment, physical disabilities, seizure disorders, diabetes, and mental illness. Some service animals have been trained as a screening tool for cancer. We review a case involving a 6-year-old female with a history of mast cell mediator release and immediate hypersensitivity due to the urticaria pigmentosa variant of cutaneous mastocytosis who underwent a cystourethroscopy. Her service dog, JJ, who would alert to mast cell mediator release, was used throughout the perioperative course as a means of anxiolysis and comfort and to monitor for mast cell mediator release. This case presents an example of a service dog used in a family-care model in the field of anesthesiology and provides a unique example of using a service dog as an additional monitor to alert the care team for impending mast cell mediator release. Shannon Tew and Brad M. Taicher Copyright © 2016 Shannon Tew and Brad M. Taicher. All rights reserved. Multiscale Fluctuation Features of the Dynamic Correlation between Bivariate Time Series Sun, 23 Oct 2016 09:23:48 +0000 http://www.hindawi.com/journals/mpe/2016/4742060/ The fluctuation of the dynamic correlation between bivariate time series has some special features on the time-frequency domain. In order to study these fluctuation features, this paper built the dynamic correlation network models using two kinds of time series as sample data. After studying the dynamic correlation networks at different time-scales, we found that the correlation between time series is a dynamic process. The correlation is strong and stable in the long term, but it is weak and unstable in the short and medium term. There are key correlation modes which can effectively indicate the trend of the correlation. The transmission characteristics of correlation modes show that it is easier to judge the trend of the fluctuation of the correlation between time series from the short term to long term. The evolution of media capability of the correlation modes shows that the transmission media in the long term have higher value to predict the trend of correlation. This work does not only propose a new perspective to analyze the correlation between time series but also provide important information for investors and decision makers. Meihui Jiang, Xiangyun Gao, Haizhong An, Xiaoliang Jia, and Xiaoqi Sun Copyright © 2016 Meihui Jiang et al. All rights reserved. Real-Time Multifault Rush Repairing Strategy Based on Utility Theory and Multiagent System in Distribution Networks Sun, 23 Oct 2016 09:12:57 +0000 http://www.hindawi.com/journals/mpe/2016/3891595/ The problem of multifault rush repair in distribution networks (DNs) is a multiobjective dynamic combinatorial problem with topology constraints. The problem consists of archiving an optimal faults’ allocation strategy to squads and an admissible multifault rush repairing strategy with coordinating switch operations. In this article, the utility theory is introduced to solve the first problem and a new discrete bacterial colony chemotaxis (DBCC) algorithm is proposed for the second problem to determine the optimal sequence for each squad to repair faults and the corresponding switch operations. The above solution is called the two-stage approach. Additionally, a double mathematical optimization model based on the fault level is proposed in the second stage to minimize the outage loss and total repairing time. The real-time adjustment multiagent system (RA-MAS) is proposed to provide facility to achieve online multifault rush repairing strategy in DNs when there are emergencies after natural disasters. The two-stage approach is illustrated with an example from a real urban distribution network and the simulation results show the effectiveness of the two-stage approach. Zhao Hao, Zhang Jing, Lu Zhi-gang, Yang Li-jun, and Cheng Hui-lin Copyright © 2016 Zhao Hao et al. All rights reserved. Metastatic Prostate Cancer of Hand Sun, 23 Oct 2016 09:08:50 +0000 http://www.hindawi.com/journals/crior/2016/1472932/ Soft tissue metastases of prostate cancer to other sites are extremely rare, and, to our best knowledge, there have been no reports of metastasis to soft tissue of the hand. A 63-year-old man was diagnosed with prostatic cancer. During treatment, bone and soft tissue metastases to the right hand, appearing in the first web space, were observed. The tumor was resected, along with both the first and second metacarpal bones. The thumb was reconstructed by pollicization of the remaining index finger, enabling the patient to use the pollicized thumb for activities of daily living. This is the first case report of prostate cancer metastasizing to the soft tissue in hand. After wide resection, pollicization was able to reconstruct a functional hand and thumb. Akihito Nagano, Takatoshi Ohno, Koji Oshima, Daichi Ishimaru, Yutaka Nishimoto, Yoshiyuki Ohno, Akihiro Hirakawa, Tatsuhiko Miyazaki, and Haruhiko Akiyama Copyright © 2016 Akihito Nagano et al. All rights reserved. NLRP3 Inflammasome Activation in the Brain after Global Cerebral Ischemia and Regulation by 17β-Estradiol Sun, 23 Oct 2016 08:56:48 +0000 http://www.hindawi.com/journals/omcl/2016/8309031/ 17β-Estradiol (E2) is a well-known neuroprotective factor in the brain. Recently, our lab demonstrated that the neuroprotective and cognitive effects of E2 require mediation by the estrogen receptor (ER) coregulator protein and proline-, glutamic acid-, and leucine-rich protein 1 (PELP1). In the current study, we examined whether E2, acting via PELP1, can exert anti-inflammatory effects in the ovariectomized rat and mouse hippocampus to regulate NLRP3 inflammasome activation after global cerebral ischemia (GCI). Activation of the NLRP3 inflammasome pathway and expression of its downstream products, cleaved caspase-1 and IL-1β, were robustly increased in the hippocampus after GCI, with peak levels observed at 6-7 days. Expression of P2X7 receptor, an upstream regulator of NLRP3, was also increased after GCI. E2 markedly inhibited NLRP3 inflammasome pathway activation, caspase-1, and proinflammatory cytokine production, as well as P2X7 receptor expression after GCI (at both the mRNA and protein level). Intriguingly, the ability of E2 to exert these anti-inflammatory effects was lost in PELP1 forebrain-specific knockout mice, indicating a key role for PELP1 in E2 anti-inflammatory signaling. Collectively, our study demonstrates that NLRP3 inflammasome activation and proinflammatory cytokine production are markedly increased in the hippocampus after GCI, and that E2 signaling via PELP1 can profoundly inhibit these proinflammatory effects. Roshni Thakkar, Ruimin Wang, Gangadhara Sareddy, Jing Wang, Dharma Thiruvaiyaru, Ratna Vadlamudi, Quanguang Zhang, and Darrell Brann Copyright © 2016 Roshni Thakkar et al. All rights reserved. Morphological Study and Dielectric Behavior of Nonisothermally Crystallized Poly(ethylene naphthalate) Nanocomposites as a Function of Graphene Content Sun, 23 Oct 2016 08:56:43 +0000 http://www.hindawi.com/journals/jnm/2016/9846102/ Morphological evolution and dielectric properties of poly(ethylene naphthalate)- (PEN-) graphene nanocomposites nonisothermally crystallized have been investigated. PEN-graphene nanocomposites containing 0.01, 0.025, 0.05, 0.075, and 0.1 wt% of graphene were prepared by melt blending in a mini twin screw extruder. The results showed that graphene exhibited a superior influence on morphological and conformational structure of PEN during nonisothermal crystallization at low graphene contents. Crystallization temperature () was found to be increased up to 18°C supporting the high nucleating activity of graphene layers. Wide angle X-ray diffraction (WAXD) and Fourier Transform Infrared Spectroscopy (FTIR) indicated that graphene modifies the conformation of PEN chains promoting crystallinity and favoring the evolution from to crystalline form with homogeneous lamellar thickness. It may be attributed to the structural similarity between naphthalene rings and graphene structure and to - interactions during nucleation. Dielectric behavior was found to be a function of graphene content where the nanocomposites changed from dielectric to low conducting material when passing from 0.075 to 0.1 wt% of graphene content. This phenomenon permits having a wide range of properties to fit a wide variety of applications required to store electrical energy of low voltage. Adriana B. Espinoza-Martinez, Felipe Avalos-Belmontes, Luis F. Ramos-de Valle, Pedro A. Espinoza-Martinez, Carlos A. Avila-Orta, Florentino Soriano-Corral, José M. Mata-Padilla, and María M. Tellez-Rosas Copyright © 2016 Adriana B. Espinoza-Martinez et al. All rights reserved. On the Modelling of Context-Aware Security for Mobile Devices Sun, 23 Oct 2016 08:51:09 +0000 http://www.hindawi.com/journals/misy/2016/8743504/ Security management in wireless networks has to deal with the changing character of the environment, which can further lead to decision making problem for unexpected events. Among a huge list of devices, the mobile ones are especially vulnerable to this situation. The solution for adapting systems and applications to dynamic environments can be context-aware description of the user actions, which gives a possibility to take into account the factors that influence these actions. In the article, we propose a context-aware security adjusting model, which is based on proposition logic and incorporates mechanisms that assist in the reasoning process. The main benefits that differentiate our approach from similar ones are a formal representation of the model, the usage of the whole spectrum of context attributes, the detection and analysis of contextual data integrity, and conflicting rules’ eradication capability. All these traits transcribe into a more effective way of adjusting security measures in accordance with existing circumstances. To illustrate the proposed approach, we present the case study of context-aware security management for mobile devices. Tomasz Zurek, Michail Mokkas, and Bogdan Ksiezopolski Copyright © 2016 Tomasz Zurek et al. All rights reserved. Synthesis and Antimicrobial Activity of N-(6-Carboxyl Cyclohex-3-ene Carbonyl) Chitosan with Different Degrees of Substitution Sun, 23 Oct 2016 08:33:38 +0000 http://www.hindawi.com/journals/ijcc/2016/6046232/ Five products of -(6-carboxyl cyclohex-3-ene carbonyl) chitosan as antimicrobial agents were prepared by reaction of chitosan with tetrahydrophthalic anhydride (THPA) at different degrees of substitution (DS). The antimicrobial activity was evaluated against four plant bacteria and eight fungi. The results proved that the inhibitory property and water solubility of the synthesized chitosan derivatives, with increase of the DS, exhibited a remarkable improvement over chitosan. The product with a DS of 0.40 was the most active one with MIC of 510, 735, 240, and 385 mg/L against Erwinia carotovora, Ralstonia solanacearum, Rhodococcus fascians, and Rhizobium radiobacter, respectively, and also in mycelial growth inhibition against Alternaria alternata (EC50 = 683 mg/L), Botrytis cinerea (EC50 = 774 mg/L), Botryodiplodia theobromae (EC50 = 501 mg/L), Fusarium oxysporum (EC50 = 500 mg/L), F. solani (EC50 = 260 mg/L), Penicillium digitatum (EC50 = 417 mg/L), Phytophthora infestans (EC50 = 298 mg/L), and Sclerotinia sclerotiorum (EC50 = 763 mg/L). These compounds based on a biodegradable and biocompatible chitosan could be used as potentially antimicrobial agents in crop protection instead of hazardous synthetic pesticides. Mohamed E. I. Badawy and Entsar I. Rabea Copyright © 2016 Mohamed E. I. Badawy and Entsar I. Rabea. All rights reserved. Congenital Vitelline Band Causing Intestinal Obstruction in an Adult with a Double Inferior Vena Cava Sun, 23 Oct 2016 08:09:17 +0000 http://www.hindawi.com/journals/crira/2016/4015408/ Introduction. Vitelline artery remnants are rare causes of intra-abdominal bands leading to bowel obstruction. These bands may be associated with Meckel’s diverticulum. Double inferior vena cava (IVC) is a rare presentation and is usually identified incidentally. Case Presentation. A sixty-year-old male presented with progressive vomiting for five days and he was clinically diagnosed with intestinal obstruction. Plain X-ray abdomen showed evidence of small bowel obstruction. CT scan of the abdomen revealed dilated small bowel loops with a small outpouching in the distal ileum with a band like structure attached to it. In the CT, left sided patent IVC draining into the left renal vein was identified. Left external iliac vein was in continuity with the left IVC. Left internal iliac vein was draining into the right IVC. Exploratory laparotomy revealed a Meckel’s diverticulum with a band identified as the vitelline remnant attached to its apex and inserting at the anterior abdominal wall near the umbilicus. Discussion. Meckel’s diverticulum with vitelline bands, although rare, should be borne in mind in adult patients with intestinal obstruction. Identification of this anomaly can be difficult in imaging studies. Presence of double IVC should be mentioned in the imaging findings to prevent possible catastrophic complications during surgery. Mihiri Wettasinghe, Kumari Pussepitiya, Bandula Samarasinghe, and Nuwan Wickramasinghe Copyright © 2016 Mihiri Wettasinghe et al. All rights reserved. Inflammatory Biomarkers in Cancer Sun, 23 Oct 2016 07:56:16 +0000 http://www.hindawi.com/journals/mi/2016/7282797/ Tomoki Nakamura, Czar L. Gaston, Krishna Reddy, Shintaro Iwata, and Jun Nishio Copyright © 2016 Tomoki Nakamura et al. All rights reserved. Indoxyl Sulfate Induces Mesangial Cell Proliferation via the Induction of COX-2 Sun, 23 Oct 2016 07:40:35 +0000 http://www.hindawi.com/journals/mi/2016/5802973/ Indoxyl sulfate (IS) is one of important uremic toxins and is markedly accumulated in the circulation of end stage renal disease (ESRD) patients, which might contribute to the damage of residual nephrons and progressive loss of residual renal function (RRF). Thus this study was undertaken to investigate the role of IS in modulating mesangial cell (MC) proliferation and the underlying mechanism. The proliferation of MCs induced by IS was determined by cell number counting, DNA synthase rate, and cell cycle phase analysis. COX-2 expression was examined by Western blotting and qRT-PCR, and a specific COX-2 inhibitor NS398 was applied to define its role in IS-induced MC proliferation. Following IS treatment, MCs exhibited increased total cell number, DNA synthesis rate, and number of cells in S and G2 phases paralleled with the upregulation of cyclin A2 and cyclin D1. Next, we found an inducible inflammation-related enzyme COX-2 was remarkably enhanced by IS, and the inhibition of COX-2 by NS398 significantly blocked IS-induced MC proliferation in line with a blockade of PGE2 production. These findings indicated that IS could induce MC proliferation via a COX-2-mediated mechanism, providing new insights into the understanding and therapies of progressive loss of RRF in ESRD. Shuzhen Li, Sijie Cheng, Zhenzhen Sun, Harr-keshauve Mungun, Wei Gong, Jing Yu, Weiwei Xia, Yue Zhang, Songming Huang, Aihua Zhang, and Zhanjun Jia Copyright © 2016 Shuzhen Li et al. All rights reserved. Differential Gene Expression during Larval Metamorphic Development in the Pearl Oyster, Pinctada fucata, Based on Transcriptome Analysis Sun, 23 Oct 2016 07:38:51 +0000 http://www.hindawi.com/journals/ijg/2016/2895303/ P. fucata experiences a series of transformations in appearance, from swimming larvae to sessile juveniles, during which significant changes in gene expression likely occur. Thus, P. fucata could be an ideal model in which to study the molecular mechanisms of larval metamorphosis during development in invertebrates. To study the molecular driving force behind metamorphic development in larvae of P. fucata, transcriptomes of five larval stages (trochophore, D-shape, umbonal, eyespots, and spats) were sequenced using an Illumina HiSeq™ 2000 system and assembled and characterized with the transcripts of six tissues. As a result, a total of 174,126 unique transcripts were assembled and 60,999 were annotated. The number of unigenes varied among the five larval stages. Expression profiles were distinctly different between trochophore, D-shape, umbonal, eyespots, and spats larvae. As a result, 29 expression trends were sorted, of which eight were significant. Among others, 80 development-related, differentially expressed unigenes (DEGs) were identified, of which the majority were homeobox-containing genes. Most DEGs occurred among trochophore, D-shaped, and UES (umbonal, eyespots, and spats) larvae as verified by qPCR. Principal component analysis (PCA) also revealed significant differences in expression among trochophore, D-shaped, and UES larvae with ten transcripts identified but no matching annotations. Haimei Li, Bo Zhang, Guiju Huang, Baosuo Liu, Sigang Fan, Dongling Zhang, and Dahui Yu Copyright © 2016 Haimei Li et al. All rights reserved. Studying Maximum Plantar Stress per Insole Design Using Foot CT-Scan Images of Hyperelastic Soft Tissues Sun, 23 Oct 2016 07:35:26 +0000 http://www.hindawi.com/journals/abb/2016/8985690/ The insole shape and the resulting plantar stress distribution have a pivotal impact on overall health. In this paper, by Finite Element Method, maximum stress value and stress distribution of plantar were studied for different insoles designs, which are the flat surface and the custom-molded (conformal) surface. Moreover, insole thickness, heel’s height, and different materials were used to minimize the maximum stress and achieve the most uniform stress distribution. The foot shape and its details used in this paper were imported from online CT-Scan images. Results show that the custom-molded insole reduced maximum stress 40% more than the flat surface insole. Upon increase of thickness in both insole types, stress distribution becomes more uniform and maximum stress value decreases up to 10%; however, increase of thickness becomes ineffective above a threshold of 1 cm. By increasing heel height (degree of insole), maximum stress moves from heel to toes and becomes more uniform. Therefore, this scenario is very helpful for control of stress in 0.2° to 0.4° degrees for custom-molded insole and over 1° for flat insole. By changing the material of the insole, the value of maximum stress remains nearly constant. The custom-molded (conformal) insole which has 0.5 to 1 cm thickness and 0.2° to 0.4° degrees is found to be the most compatible form for foot. Ali Sarikhani, Abbas Motalebizadeh, Sasan Asiaei, and Babak Kamali Doost Azad Copyright © 2016 Ali Sarikhani et al. All rights reserved. Performance of Seismic Restrainer with SMA Springs for Sliding Isolation of Single-Layer Spherical Lattice Shells Sun, 23 Oct 2016 07:29:10 +0000 http://www.hindawi.com/journals/sv/2016/9218317/ The seismic response of a single-layer spherical lattice shell controlled by restorable sliding isolator is studied under different seismic excitations. The isolation system consists of flat steel-Teflon sliding isolators and superelastic SMA spring restrainers. The NiTi-SMA is used to fabricate helical spring for recentering control of the isolation system. In the first step of this investigation, the configuration scheme and functioning mechanism of a novel SMA spring restrainer are introduced briefly. Then, realistic mechanical behavior of large-scale superelastic NiTi helical spring is studied through a set of cyclic experimental tests. According to the obtained hysteresis loops, a mechanical model combining multilinear model and hysteresis model is developed to simulate the overall response of the SMA-based seismic restrainer. Besides, the sliding isolator is evaluated using a bilinear force-displacement hysteresis model. Finally, a 60 m span single-layer spherical lattice shell with substructure is modeled with finite element program. Nonlinear time history analyses of the controlled and uncontrolled lattice shell are performed considering multidimensional seismic inputs. The study shows that the seismic response of the controlled lattice shell can be effectively reduced by using isolation and control devices. Furthermore, the seismic response of the isolation system such as peak displacement and residual displacement can be effectively controlled by using the developed SMA spring restrainers. Peng Zhuang and Wenting Wang Copyright © 2016 Peng Zhuang and Wenting Wang. All rights reserved. MMP8 Is Increased in Lesions and Blood of Acne Inversa Patients: A Potential Link to Skin Destruction and Metabolic Alterations Sun, 23 Oct 2016 07:20:10 +0000 http://www.hindawi.com/journals/mi/2016/4097574/ Acne inversa (AI; also designated as hidradenitis suppurativa) is a chronic inflammatory disease with still unknown pathogenesis that affects the intertriginous skin of perianal, inguinal, and axillary sites. It leads to painful nodules, abscesses, and fistulas with malodorous secretion and is frequently associated with metabolic alterations. Here, we demonstrate that one of the most highly upregulated molecules in AI lesions is matrix metalloproteinase 8 (MMP8), an enzyme specialized in the degradation of extracellular matrix components and the HDL component apolipoprotein A-I. Granulocytes, which were present in AI lesions, secreted high amounts of MMP8 especially after TNF-α stimulation. Furthermore, activated fibroblasts but not keratinocytes were found to express MMP8. The high lesional MMP8 levels were accompanied by elevated blood levels that positively correlated with TNF-α blood levels and disease severity assessed by Sartorius score, especially with the number of regions with inflammatory nodules/abscesses and fistulas. Additionally, we found a negative correlation between blood MMP8 and HDL-cholesterol levels, suggesting a contributory role of MMP8 in metabolic alterations in AI. In summary, we demonstrate elevated MMP8 levels in AI lesions, suggest their role in skin destruction and metabolic alterations, and recommend the use of MMP8 as blood biomarker for AI disease activity assessment. Athanasia Tsaousi, Ellen Witte, Katrin Witte, Hans-Joachim Röwert-Huber, Hans-Dieter Volk, Wolfram Sterry, Kerstin Wolk, Sylke Schneider-Burrus, and Robert Sabat Copyright © 2016 Athanasia Tsaousi et al. All rights reserved. Low-Grade Appendiceal Mucinous Neoplasm Involving the Endometrium and Presenting with Mucinous Vaginal Discharge Sun, 23 Oct 2016 06:57:24 +0000 http://www.hindawi.com/journals/criog/2016/6841989/ Primary appendiceal mucinous lesions are uncommon and represent a spectrum from nonneoplastic mucous retention cysts to invasive adenocarcinoma. Low-grade appendiceal mucinous neoplasms (LAMNs) represent an intermediate category on this spectrum and can be classified according to whether or not they are confined to the appendix. Although LAMNs are frequently confined to the appendix, they can also spread to the peritoneum and clinically progress as pseudomyxoma peritonei (i.e., mucinous ascites). Thus, the appropriate classification of appendiceal primary neoplasia is essential for prognosis and influences clinical management. In addition, the precise classification, management, and clinical outcome of patients with disseminated peritoneal disease remain controversial. Here, we report an unusual case of LAMN with pseudomyxoma peritonei that initially presented with mucinous and bloody vaginal discharge. Pathological evaluation revealed low-grade appendiceal mucinous neoplasm with secondary involvement of the peritoneum, ovaries, and endometrial surface. Therefore, LAMN should be considered in the differential diagnosis of mucinous vaginal discharge. Vera Vavinskaya, Joel M. Baumgartner, Albert Ko, Cheryl C. Saenz, and Mark A. Valasek Copyright © 2016 Vera Vavinskaya et al. All rights reserved. Color-Matching and Blending-Effect of Universal Shade Bulk-Fill-Resin-Composite in Resin-Composite-Models and Natural Teeth Sun, 23 Oct 2016 06:31:42 +0000 http://www.hindawi.com/journals/bmri/2016/4183432/ Objectives. To assess visually color-matching and blending-effect (BE) of a universal shade bulk-fill-resin-composite placed in resin-composite-models with different shades and cavity sizes and in natural teeth (extracted and patients’ teeth). Materials and Methods. Resin-composite-discs (10 mm × 1 mm) were prepared of universal shade composite and resin-composite of shades: A1, A2, A3, A3.5, and A4. Spectrophotometric-color-measurement was performed to calculate color-difference () between the universal shade and shaded-resin-composites discs and determine their translucency-parameter (TP). Visual assessment was performed by seven normal-color-vision-observers to determine the color-matching between the universal shade and each shade, under Illuminant D65. Color-matching visual scoring (VS) values were expressed numerically (1–5): 1: mismatch/totally unacceptable, 2: Poor-Match/hardly acceptable, 3: Good-Match/acceptable, 4: Close-Match/small-difference, and 5: Exact-Match/no-color-difference. Occlusal cavities of different sizes were prepared in teeth-like resin-composite-models with shades A1, A2, A3, A3.5, and A4. The cavities were filled by the universal shade composite. The same scale was used to score color-matching between the fillings and composite-models. BE was calculated as difference in mean-visual-scores in models and that of discs. Extracted teeth with two different class I-cavity sizes as well as ten patients’ lower posterior molars with occlusal caries were prepared, filled by universal shade composite, and assessed similarly. Results. In models, the universal shade composite showed close matching in the different cavity sizes and surrounding shades () (BE = 0.6–2.9 in small cavities and 0.5–2.8 in large cavities). In extracted teeth, there was good-to-close color-matching (VS = 3.7–4.4 in small cavities, BE = 2.5–3.2) (VS = 3–3.5, BE = 1.8–2.3 in large cavities). In patients’ molars, the universal shade composite showed good-matching (VS = 3–3.3, BE = −0.9–2.1). Conclusions. Color-matching of universal shade resin-composite was satisfactory rather than perfect in patients’ teeth. Rasha M. Abdelraouf and Nour A. Habib Copyright © 2016 Rasha M. Abdelraouf and Nour A. Habib. All rights reserved. Pilot Validation Study: Canadian Global Rating Scale for Colonoscopy Services Thu, 20 Oct 2016 16:15:00 +0000 http://www.hindawi.com/journals/cjgh/2016/6982739/ Background. The United Kingdom Global Rating Scale (GRS-UK) measures unit-level quality metrics processes in digestive endoscopy. We evaluated the psychometric properties of its Canadian version (GRS-C), endorsed by the Canadian Association of Gastroenterology (CAG). Methods. Prospective data collection at three Canadian endoscopy units assessed GRS-C validity, reliability, and responsiveness to change according to responses provided by physicians, endoscopy nurses, and administrative personnel. These responses were compared to national CAG endoscopic quality guidelines and GRS-UK statements. Results. Most respondents identified the overarching theme each GRS-C item targeted, confirming face validity. Content validity was suggested as 18 out of 23 key CAG endoscopic quality indicators (78%, 95% CI: 56–93%) were addressed in the GRS-C; statements not included pertained to educational programs and competency monitoring. Concordance ranged 75–100% comparing GRS-C and GRS-UK ratings. Test-retest reliability Kappa scores ranged 0.60–0.83, while responsiveness to change scores at 6 months after intervention implementations were greater () in two out of three units. Conclusion. The GRS-C exhibits satisfactory metrics, supporting its use in a national quality initiative aimed at improving processes in endoscopy units. Data collection from more units and linking to actual patient outcomes are required to ensure that GRS-C implementation facilitates improved patient care. Stéphanie Carpentier, Nour Sharara, Alan N. Barkun, Sara El Ouali, Myriam Martel, and Maida J. Sewitch Copyright © 2016 Stéphanie Carpentier et al. All rights reserved. Pomegranate-Derived Polyphenols Reduce Reactive Oxygen Species Production via SIRT3-Mediated SOD2 Activation Thu, 20 Oct 2016 16:13:13 +0000 http://www.hindawi.com/journals/omcl/2016/2927131/ Pomegranate-derived polyphenols are expected to prevent life-style related diseases. In this study, we evaluated the ability of 8 pomegranate-derived polyphenols, along with other polyphenols, to augment SIRT3, a mammalian SIR2 homolog localized in mitochondria. We established a system for screening foods/food ingredients that augment the SIRT3 promoter in Caco-2 cells and identified 3 SIRT3-augmenting pomegranate-derived polyphenols (eucalbanin B, pomegraniin A, and eucarpanin T1). Among them, pomegraniin A activated superoxide dismutase 2 (SOD2) through SIRT3-mediated deacetylation, thereby reducing intracellular reactive oxygen species. The other SIRT3-augmenting polyphenols tested also activated SOD2, suggesting antioxidant activity. Our findings clarify the underlying mechanisms involved in the antioxidant activity of pomegraniin A. Chong Zhao, Takenori Sakaguchi, Kosuke Fujita, Hideyuki Ito, Norihisa Nishida, Akifumi Nagatomo, Yukimasa Tanaka-Azuma, and Yoshinori Katakura Copyright © 2016 Chong Zhao et al. All rights reserved. Evaluating Correlations and Development of Meteorology Based Yield Forecasting Model for Strawberry Thu, 20 Oct 2016 16:08:30 +0000 http://www.hindawi.com/journals/amete/2016/9525204/ California state is among the leading producers of strawberries in the world. The value of the California strawberry crop is approximately $2.6 billion, which makes it one of the most valuable fruit crops for the state and nation’s economy. California’s weather provides ideal conditions for strawberry production and changes in weather pattern could have a significant impact on strawberry fruit production. Evaluating relationships between meteorological parameters and strawberry yield can provide valuable information and early indications of yield forecasts that growers can utilize to their advantage. Objectives of this paper were to evaluate correlations of meteorological parameters on strawberry yield for Santa Maria region and to develop meteorology based empirical yield forecasting models for strawberries. Results showed significant correlation between meteorological parameters and strawberry yield and provided a basis for yield forecasting with lead time. Results from empirical models showed that cross-validated yields were closely associated with observed yield with lead time of 2 to 5 months. Overall, this study showed great potential in developing meteorology based yield forecast using principal components. This study only looked at meteorology based yield forecasts. Skills of these models can be further improved by adding physiological parameters of strawberry to existing models for strawberry. Tapan B. Pathak, Surendra Dara, and Andre Biscaro Copyright © 2016 Tapan B. Pathak et al. All rights reserved. Monitoring Minimal Residual Disease in the Myeloproliferative Neoplasms: Current Applications and Emerging Approaches Thu, 20 Oct 2016 16:08:04 +0000 http://www.hindawi.com/journals/bmri/2016/7241591/ The presence of acquired mutations within the JAK2, CALR, and MPL genes in the majority of patients with myeloproliferative neoplasms (MPN) affords the opportunity to utilise these mutations as markers of minimal residual disease (MRD). Reduction of the mutated allele burden has been reported in response to a number of therapeutic modalities including interferon, JAK inhibitors, and allogeneic stem cell transplantation; novel therapies in development will also require assessment of efficacy. Real-time quantitative PCR has been widely adopted for recurrent point mutations with assays demonstrating the specificity, sensitivity, and reproducibility required for clinical utility. More recently, approaches such as digital PCR have demonstrated comparable, if not improved, assay characteristics and are likely to play an increasing role in MRD monitoring. While next-generation sequencing is increasingly valuable as a tool for diagnosis of MPN, its role in the assessment of MRD requires further evaluation. Karl Haslam and Stephen E. Langabeer Copyright © 2016 Karl Haslam and Stephen E. Langabeer. All rights reserved. Tetrastigma diepenhorstii (Miq.) Latiff (Vitaceae), a New Host of Rafflesia tuan-mudae Becc. (Rafflesiaceae) in Borneo Thu, 20 Oct 2016 16:07:40 +0000 http://www.hindawi.com/journals/jb/2016/3952323/ Rafflesia tuan-mudae Becc. (Rafflesiaceae) is endemic to Borneo and was recorded from Sarawak and probably Kalimantan. Previous records showed that Tetrastigma rafflesiae (Miq.) Planch. (Vitaceae) is the only host plant for R. tuan-mudae. In this study the host plants were collected each time R. tuan-mudae was observed or collected. Out of 20 Tetrastigma specimens collected infected by R. tuan-mudae, 14 were identified as T. diepenhorstii (Miq.) Latiff while 6 belonged to T. rafflesiae. Therefore, a new host for R. tuan-mudae is recorded and descriptions for each host are presented. Wan Nuur Fatiha Wan Zakaria, Aida Shafreena Ahmad Puad, Connie Geri, Ramlah Zainudin, and Abdul Latiff Copyright © 2016 Wan Nuur Fatiha Wan Zakaria et al. All rights reserved. Neuroinflammation in Autism: Plausible Role of Maternal Inflammation, Dietary Omega 3, and Microbiota Thu, 20 Oct 2016 16:06:52 +0000 http://www.hindawi.com/journals/np/2016/3597209/ Several genetic causes of autism spectrum disorder (ASD) have been identified. However, more recent work has highlighted that certain environmental exposures early in life may also account for some cases of autism. Environmental insults during pregnancy, such as infection or malnutrition, seem to dramatically impact brain development. Maternal viral or bacterial infections have been characterized as disruptors of brain shaping, even if their underlying mechanisms are not yet fully understood. Poor nutritional diversity, as well as nutrient deficiency, is strongly associated with neurodevelopmental disorders in children. For instance, imbalanced levels of essential fatty acids, and especially polyunsaturated fatty acids (PUFAs), are observed in patients with ASD and other neurodevelopmental disorders (e.g., attention deficit hyperactivity disorder (ADHD) and schizophrenia). Interestingly, PUFAs, and specifically n-3 PUFAs, are powerful immunomodulators that exert anti-inflammatory properties. These prenatal dietary and immunologic factors not only impact the fetal brain, but also affect the microbiota. Recent work suggests that the microbiota could be the missing link between environmental insults in prenatal life and future neurodevelopmental disorders. As both nutrition and inflammation can massively affect the microbiota, we discuss here how understanding the crosstalk between these three actors could provide a promising framework to better elucidate ASD etiology. Charlotte Madore, Quentin Leyrolle, Chloé Lacabanne, Anouk Benmamar-Badel, Corinne Joffre, Agnes Nadjar, and Sophie Layé Copyright © 2016 Charlotte Madore et al. All rights reserved. Computational Analysis of Damaging Single-Nucleotide Polymorphisms and Their Structural and Functional Impact on the Insulin Receptor Thu, 20 Oct 2016 16:06:18 +0000 http://www.hindawi.com/journals/bmri/2016/2023803/ Single-nucleotide polymorphisms (SNPs) associated with complex disorders can create, destroy, or modify protein coding sites. Single amino acid substitutions in the insulin receptor (INSR) are the most common forms of genetic variations that account for various diseases like Donohue syndrome or Leprechaunism, Rabson-Mendenhall syndrome, and type A insulin resistance. We analyzed the deleterious nonsynonymous SNPs (nsSNPs) in INSR gene based on different computational methods. Analysis of INSR was initiated with PROVEAN followed by PolyPhen and I-Mutant servers to investigate the effects of 57 nsSNPs retrieved from database of SNP (dbSNP). A total of 18 mutations that were found to exert damaging effects on the INSR protein structure and function were chosen for further analysis. Among these mutations, our computational analysis suggested that 13 nsSNPs decreased protein stability and might have resulted in loss of function. Therefore, the probability of their involvement in disease predisposition increases. In the lack of adequate prior reports on the possible deleterious effects of nsSNPs, we have systematically analyzed and characterized the functional variants in coding region that can alter the expression and function of INSR gene. In silico characterization of nsSNPs affecting INSR gene function can aid in better understanding of genetic differences in disease susceptibility. Zabed Mahmud, Syeda Umme Fahmida Malik, Jahed Ahmed, and Abul Kalam Azad Copyright © 2016 Zabed Mahmud et al. All rights reserved. CAA of an Air-Cooling System for Electronic Devices Thu, 20 Oct 2016 14:48:48 +0000 http://www.hindawi.com/journals/aav/2016/4785389/ This paper presents the workflow and the results of fluid dynamics and aeroacoustic simulations for an air-cooling system as used in electronic devices. The setup represents a generic electronic device with several electronic assemblies with forced convection cooling by two axial fans. The aeroacoustic performance is computed using a hybrid method. In a first step, two unsteady CFD simulations using the Unsteady Reynolds-Averaged Navier-Stokes simulation with Shear Stress Transport (URANS-SST) turbulence model and the Scale Adaptive Simulation with Shear Stress Transport (SAS-SST) models were performed. Based on the unsteady flow results, the acoustic source terms were calculated using Lighthill’s acoustic analogy. Propagation of the flow-induced sound was computed using the Finite Element Method. Finally, the results of the acoustic simulation are compared with measurements and show good agreement. Sven Münsterjohann, Jens Grabinger, Stefan Becker, and Manfred Kaltenbacher Copyright © 2016 Sven Münsterjohann et al. All rights reserved. Endothelium-Dependent Vasorelaxant Effects of Dealcoholized Wine Powder of Wild Grape (Vitis coignetiae) in the Rat Thoracic Aorta Thu, 20 Oct 2016 14:45:30 +0000 http://www.hindawi.com/journals/ecam/2016/6846084/ The vasorelaxant effects of dealcoholized wild grape (Vitis coignetiae) wine were investigated with isolated rat thoracic aorta. In our present study, we demonstrate that wild grape wine powder (WGWP) induced relaxation of aortic rings preconstricted with norepinephrine in a dose-dependent manner (at concentrations ranging from 0.1 to 1 mg/mL). The vasorelaxant effect of WGWP was dependent on intact endothelia, which was attenuated by incubation with inhibitors of endothelium-derived relaxing factors, such as -nitro-L-arginine (nitric oxide synthase inhibitor), methylene blue (guanylate cyclase inhibitor), and indomethacin (cyclooxygenase inhibitor). Moreover, treatment with WGWP and atropine (muscarinic receptor antagonist) or diphenylhydramine (histamine receptor antagonist) significantly inhibited endothelium-dependent vasorelaxation. Our results suggest that WGWP induces relaxation in rat aortic rings in an endothelium-dependent manner. Results further indicate that this effect occurs via nitric oxide-cGMP pathway and prostacyclin-cAMP pathway through a muscarinic receptor and histamine receptor. Sang Keun Ha, Ho-Young Park, Mee-Ra Ryu, Yoonsook Kim, and Yongkon Park Copyright © 2016 Sang Keun Ha et al. All rights reserved. Recent Advances in Biomedical Applications Thu, 20 Oct 2016 14:38:58 +0000 http://www.hindawi.com/journals/abb/2016/6808695/ Alberto Borboni, Tadeusz Mikołajczyk, and Vera Murgul Copyright © 2016 Alberto Borboni et al. All rights reserved. Trichotillomania as a Manifestation of Dementia Thu, 20 Oct 2016 14:37:21 +0000 http://www.hindawi.com/journals/crips/2016/9782702/ Pathological hair-pulling or trichotillomania, which is commonly associated with anxiety and depression, obsessive-compulsive disorder, and neurodevelopmental disorders, has been rarely associated with dementing illnesses. Investigators have not clarified the neural correlates and treatment of trichotillomania in dementia. We report a patient who developed an early-onset cognitive decline with genetic, cerebrospinal fluid biomarker and structural and functional neuroimaging studies consistent with Alzheimer’s disease. Eight years into her disease, she developed severe, repetitive hair-pulling behavior leading to marked hair loss, along with other repetitive and “frontal” behaviors. Selective serotonin reuptake inhibitors (SSRIs) were ineffective in controlling her hair-pulling behavior, which subsequently responded to quetiapine 150 mg/day. This patient and a review of the literature suggest that trichotillomania may be a compulsive-related symptom in dementias of different etiologies as they involve frontal areas and release primitive grooming behavior from frontostriatal dysfunction. Dopamine blockade, rather than SSRIs, may be effective in managing trichotillomania in dementia. Pongsatorn Paholpak and Mario F. Mendez Copyright © 2016 Pongsatorn Paholpak and Mario F. Mendez. All rights reserved. Improvement of Saemangeum Dredged Soils Using Coffee Sludge for Vegetation Soil Thu, 20 Oct 2016 14:37:09 +0000 http://www.hindawi.com/journals/amse/2016/4698561/ In Korea, a large scale national project (Saemangeum Project) has been underway that requires a huge amount of dredged soils and their reclamation. Although a lot of dredged soil is needed for reclamation, only about 10% of the dredged soil is used. For this reason, much effort should be made to extensively use the dredged soil. The objective of the study is to find reasonable ways of improving the dredged soils in the Saemangeum area so that they can be used for vegetation of land plants. In order to develop ameliorating methods, we treated silty sand samples, the representative dredged soil of Saemangeum, with mountain soil (0% and 30%), sawdust fertilizer (0% and 6%), bioameliorant (0% and 6%), and coffee sludge (3%, 6%, and 9%), measured the germination rate of bent grass, and applied the lab experiment results to the field for validation. As a result, it was verified that when a mixture of coffee sludge and sawdust fertilizer was used, the chemical and physical properties of dredged soil were significantly improved. This implies that the beneficial use of the dredged soil can be facilitated. Daehyeon Kim, Minseok Kim, Munchae Kim, and Kyungho Park Copyright © 2016 Daehyeon Kim et al. All rights reserved. A Rare Case of Glossitis due to Pasteurella multocida after a Cat Scratch Thu, 20 Oct 2016 14:36:23 +0000 http://www.hindawi.com/journals/criid/2016/2868505/ Pasteurella is one of the zoonotic pathogens that can cause variety of serious infections in animals and humans such as bacteremia, septic shock, endocarditis, meningitis, prosthetic and native valve infections, osteomyelitis, skin and soft tissue infections, abscesses, and even pneumonia with empyema. However, there have been few reports of upper respiratory involvements like tonsillitis and epiglottitis in humans. We present a case of recurrent Pasteurella glossitis after a cat scratch which has not been reported in humans. Negin Niknam, Thien Doan, and Elizabeth Revere Copyright © 2016 Negin Niknam et al. All rights reserved. A Conservative Approach to a Peripheral Ameloblastoma Thu, 20 Oct 2016 14:36:20 +0000 http://www.hindawi.com/journals/crid/2016/8254571/ Peripheral Ameloblastoma (PA) is the rarest variant of ameloblastoma. It differs from the other subtypes of ameloblastoma in its localization: it arises in the soft tissues of the oral cavity coating the tooth bearing bones. Generally, it manifests nonaggressive behavior and it can be treated with complete removal by local conservative excision. In this study we report a case of PA of the maxilla in a 78-year-old female patient and we describe the four different histopathological patterns revealed by histological examination. After local excision and diagnosis, we planned a long term follow-up: in one year no recurrence had been reported. The choice of treatment is illustrated in Discussion. Rocco Borrello, Elia Bettio, Christian Bacci, Marialuisa Valente, Stefano Sivolella, Sergio Mazzoleni, and Mario Berengo Copyright © 2016 Rocco Borrello et al. All rights reserved. Internal Carotid Artery Stenosis Presenting with Limb Shaking TIA Thu, 20 Oct 2016 14:35:47 +0000 http://www.hindawi.com/journals/crinm/2016/3656859/ Internal carotid artery (ICA) stenosis may lead to a wide range of clinical symptoms. We describe the case of a 66-year-old female who experienced a transient ischemic attack (TIA) with episodes of limb shaking caused by ICA stenosis. After epilepsy had been suspected and ruled out, studies of her left ICA showed extensive blockage as a result of atherosclerosis. Magnetic resonance angiography (MRA) revealed total occlusion of the left ICA and the patient was eventually medically managed due to the strong possibility of surgical complications. We reported this patient’s clinical course to shed light on a rare manifestation of carotid stenosis that may be confused with other diagnoses if not closely scrutinized. Awad Javaid and Mostafa Alfishawy Copyright © 2016 Awad Javaid and Mostafa Alfishawy. All rights reserved. Multiproduct Multiperiod Newsvendor Problem with Dynamic Market Efforts Thu, 20 Oct 2016 14:31:20 +0000 http://www.hindawi.com/journals/ddns/2016/7674027/ We study a multiperiod multiproduct production planning problem where the production capacity and the marketing effort on demand are both considered. The accumulative impact of marketing effort on demand is captured by the Nerlove and Arrow (N-A) advertising model. The problem is formulated as a discrete-time, finite-horizon dynamic optimization problem, which can be viewed as an extension to the classic newsvendor problem by integrating with the N-A model. A Lagrangian relaxation based solution approach is developed to solve the problem, in which the subgradient algorithm is used to find an upper bound of the solution and a feasibility heuristic algorithm is proposed to search for a feasible lower bound. Twelve kinds of instances with different problem size involving up to 50 products and 15 planning periods are randomly generated and used to test the Lagrangian heuristic algorithm. Computational results show that the proposed approach can obtain near optimal solutions for all the instances in very short CPU time, which is less than 90 seconds even for the largest instance. Jianmai Shi and Yiping Bao Copyright © 2016 Jianmai Shi and Yiping Bao. All rights reserved. Isolation, Identification, and Optimization of Culture Conditions of a Bioflocculant-Producing Bacterium Bacillus megaterium SP1 and Its Application in Aquaculture Wastewater Treatment Thu, 20 Oct 2016 14:30:56 +0000 http://www.hindawi.com/journals/bmri/2016/2758168/ A bioflocculant-producing bacterium, Bacillus megaterium SP1, was isolated from biofloc in pond water and identified by using both 16S rDNA sequencing analysis and a Biolog GEN III MicroStation System. The optimal carbon and nitrogen sources for Bacillus megaterium SP1 were 20 g L−1 of glucose and 0.5 g L−1 of beef extract at 30°C and pH 7. The bioflocculant produced by strain SP1 under optimal culture conditions was applied into aquaculture wastewater treatment. The removal rates of chemical oxygen demand (COD), total ammonia nitrogen (TAN), and suspended solids (SS) in aquaculture wastewater reached 64, 63.61, and 83.8%, respectively. The volume of biofloc (FV) increased from 4.93 to 25.97 mL L−1. The addition of Bacillus megaterium SP1 in aquaculture wastewater could effectively improve aquaculture water quality, promote the formation of biofloc, and then form an efficient and healthy aquaculture model based on biofloc technology. Liang Luo, Zhigang Zhao, Xiaoli Huang, Xue Du, Chang’an Wang, Jinnan Li, Liansheng Wang, and Qiyou Xu Copyright © 2016 Liang Luo et al. All rights reserved. Mercaptopurine Treatment in an Adult Man with Orbital and Intracranial Rosai-Dorfman Disease Thu, 20 Oct 2016 14:25:46 +0000 http://www.hindawi.com/journals/crinm/2016/1030478/ Background. Rosai-Dorfmann disease (RDD) is a rare, idiopathic non-Langerhans cell histiocytosis, affecting children and young adults, that commonly presents as painless, massive cervical lymphadenopathy with fever, weight loss, and polyclonal hypergammaglobulinemia. Cervical lymphadenopathy and extranodal involvement are the main presentations. On the contrary, ophthalmic involvement and localisation in the central nervous system are rare. Case Report. An old man was admitted to our hospital for first seizure. Brain imaging studies revealed on the left an extra-axial thickening of the dura mater with enhancement and perilesional oedema, infiltrating the sphenoorbital fissure and an isointense mass with enhancement in the orbital region with dislocation of the optic nerve. Pathological and immunohistochemistry examination of the bioptical specimen was consistent with a diagnosis of RDD. Treatment with levetiracetam and steroids was started obtaining only remission of seizures. Because of the patient refusal of the surgical debulking, therapy with mercaptopurine was started, stopping disease progression. Conclusion. So far, very few cases of extranodal RDD with multiple CNS lesions involving the orbital region have been described. Our case is significant because it is the first case in which the efficacy of mercaptopurine treatment has been documented in an adult patient with isolated ocular and intracranial RDD. Valentina Arnao, Marianna Riolo, Giovanni Savettieri, and Paolo Aridon Copyright © 2016 Valentina Arnao et al. All rights reserved. Apolipoprotein C-II Deposition Amyloidosis: A Potential Misdiagnosis as Light Chain Amyloidosis Thu, 20 Oct 2016 14:18:44 +0000 http://www.hindawi.com/journals/crin/2016/8690642/ Hereditary amyloidoses are rare and pose a diagnostic challenge. We report a case of hereditary amyloidosis associated with apolipoprotein C-II deposition in a 61-year-old female presenting with renal failure and nephrotic syndrome misdiagnosed as light chain amyloidosis. Renal biopsy was consistent with amyloidosis on microscopy; however, immunofluorescence was inconclusive for the type of amyloid protein. Monoclonal gammopathy evaluation revealed kappa light chain. Bone marrow biopsy revealed minimal involvement with amyloidosis with kappa monotypic plasma cells on flow cytometry. She was started on chemotherapy for light chain amyloidosis. She was referred to the Mayo clinic where laser microdissection and liquid chromatography mass spectrometry detected high levels of apolipoprotein C-II, making a definitive diagnosis. Apolipoprotein C-II is a component of very low-density lipoprotein and aggregates in lipid-free conditions to form amyloid fibrils. The identification of apolipoprotein C-II as the cause of amyloidosis cannot be solely made with routine microscopy or immunofluorescence. Further evaluation of biopsy specimens with laser microdissection and mass spectrometry and DNA sequencing of exons should be done routinely in patients with amyloidoses for definitive diagnosis. Our case highlights the importance of determining the subtype of amyloidosis that is critical for avoiding unnecessary therapy such as chemotherapy. Sadichhya Lohani, Emily Schuiteman, Lohit Garg, Dhiraj Yadav, and Sami Zarouk Copyright © 2016 Sadichhya Lohani et al. All rights reserved. Ileocolic Intussusception in a Leukemic Adult Patient: A Case Report and Review of the Literature Thu, 20 Oct 2016 14:16:02 +0000 http://www.hindawi.com/journals/cris/2016/3972605/ We present a rare case of intussusception in a 41-year-old man with acute myeloid leukemia without an evidence of leukemic infiltration of the bowel. The patient presented to the emergency room with right lower quadrant pain. Initially he was diagnosed with typhlitis. CT scan was done and showed ileocolic intussusception without a definitive lead point identified. Patient underwent hemicolectomy and histopathological study of the specimen did not show any leukemic infiltrate. High suspicion of intussusception should be kept in mind with leukemic patients presenting with abdominal pain. Ayoub Innabi, Wa’el Tuqan, Alia Alawneh, Alaa Saleh, Kamal Alrabi, and Lina Marei Copyright © 2016 Ayoub Innabi et al. All rights reserved. Electromagnetic Stirring versus ECAP: Morphological Comparison of Al-Si-Cu Alloys to Make the Microstructural Refinement for Use in SSM Processing Thu, 20 Oct 2016 14:14:10 +0000 http://www.hindawi.com/journals/amse/2016/9789061/ This work evaluates the morphological evolution at the semisolid state of the Al-4.0wt%Si-2.5wt%Cu alloy produced by direct chill casting under electromagnetic stirring (EMS) and by one equal channel angular pressing (ECAP) pass. The ECAP emerged as a promising technique capable of reduction and homogeneous metals microstructure imposing large deformations occurs in a matrix that contains two channels of the same cross-sectional area and forms an angle of 120°. The materials were submitted to reheating treatment in condition of 60% solid fraction at treatment times of 0, 30, and 90 s. Comparing the two cases, we have the presented ECAP process that had an excellent response to the recovery and recrystallization mechanisms, and refined microstructures ideal for thixoforming were produced. Primary particle sizes of about 45 μm and grain sizes of about 75 μm and a circularity shape factor of more than 0.60 were obtained. The low silicon alloy, Al-4.0wt%Si-2.5wt%Cu, presented excellent refinement when processed via equal channel angular pressing, presenting good morphological stability at the semisolid state, without significant changes in size or shape of the solid particles. This fully globular structure is favourable for thixoforming processes. Luis Vanderlei Torres, Luis Fernando Torres, and Eugênio José Zoqui Copyright © 2016 Luis Vanderlei Torres et al. All rights reserved. Diagnosis and Management of Cirrhosis-Related Osteoporosis Thu, 20 Oct 2016 14:11:13 +0000 http://www.hindawi.com/journals/bmri/2016/1423462/ Management of cirrhosis complications has greatly improved, increasing survival and quality of life of the patients. Despite that, some of these complications are still overlooked and scarcely treated, particularly those that are not related to the liver. This is the case of osteoporosis, the only cirrhosis complication that is not solved after liver transplantation, because bone loss often increases after immunosuppressant therapy. In this review, the definitions of bone conditions in cirrhotic patients are analyzed, focusing on the more common ones and on those that have the largest impact on this population. Risk factors, physiopathology, diagnosis, screening strategies, and treatment of osteoporosis in cirrhotic patients are discussed, presenting the more striking data on this issue. Therapies used for particular conditions, such as primary biliary cirrhosis and liver transplantation, are also presented. Lívia Alves Amaral Santos and Fernando Gomes Romeiro Copyright © 2016 Lívia Alves Amaral Santos and Fernando Gomes Romeiro. All rights reserved. Efficacy of Scutellaria baicalensis for the Treatment of Hand, Foot, and Mouth Disease Associated with Encephalitis in Patients Infected with EV71: A Multicenter, Retrospective Analysis Thu, 20 Oct 2016 14:10:05 +0000 http://www.hindawi.com/journals/bmri/2016/5697571/ This study aimed to evaluate the clinical efficacy and safety of using the traditional Chinese herbal medicine Scutellaria baicalensis for the treatment of severe HFMD in 725 patients aged >1 year in a multicenter, retrospective analysis. The patients were divided into the S. baicalensis and ribavirin groups, and the temperatures, presence or absence of skin rashes and oral lesions, nervous system (NS) involvement, and viral loads of the patients, as well as the safety of the treatments, were evaluated. The median duration of fever, median time to NS involvement, and the number of patients with oral ulcers and/or vesicles, as well as skin rashes, were decreased in the S. baicalensis group compared with the ribavirin group. In addition, the EV71 viral loads were decreased in the S. baicalensis group, suggesting that S. baicalensis exerted more potent antiviral effects compared with ribavirin. The present study demonstrated that S. baicalensis was suitable for the treatment of severe HFMD in patients aged >1 year, since it was shown to rapidly relieve fever, attenuate oral lesions and rashes, and improve NS involvement. Furthermore, it was demonstrated to be relatively safe for topical application. Hailong Lin, Jian Zhou, Kaichun Lin, Hongjiao Wang, Zunhong Liang, Xingshuai Ren, Leting Huang, and Chan Xia Copyright © 2016 Hailong Lin et al. All rights reserved. An Experimental Study on Botulinum Toxin Type A for the Treatment of Excessive Secretion after Submandibular Gland Transplantation in Rabbits Thu, 20 Oct 2016 14:02:27 +0000 http://www.hindawi.com/journals/joph/2016/7058537/ Objectives. To investigate whether botulinum toxin type A (BTXA) could control excessive secretion after submandibular gland (SMG) transplantation in rabbits and its possible mechanisms. Methods. A new SMG transplantation model was established in rabbit. 30 successfully constructed models were randomly assigned to five groups including control group and four experimental groups. Secretion outputs were used to analyze the effect of BTXA injection on excessive secretion. Hematoxylin and eosin (HE) staining, transmission electron microscopy (TEM), Western blot, and immunofluorescence were performed to analyze its possible mechanisms. Results. After BTXA injection, a significant decrease of excessive secretion after SMG transplantation was found in 2 and 4 weeks groups, but no significant effect on 12 and 24 weeks groups. HE and TEM results showed that BTXA led to morphological and ultrastructural changes of acinar cells of transplanted SMG. Western blot results suggested that BTXA decreased the aquaporin-5 (AQP5) protein expression after BTXA injection for 2 and 4 weeks. Immunofluorescence results showed that AQP5 protein was mainly expressed in the cytoplasm after BTXA injection for 2 and 4 weeks, which might indicate that BTXA promoted AQP5 expression from the cell membrane to cytoplasm. Conclusion. BTXA could effectively control excessive secretion after SMG transplantation in rabbits. Shang Xie, Hui Xu, Bo Lin, Kan Wang, Xiao-Feng Shan, and Zhi-Gang Cai Copyright © 2016 Shang Xie et al. All rights reserved. Fixed Points and Exponential Stability for Impulsive Time-Delays BAM Neural Networks via LMI Approach and Contraction Mapping Principle Thu, 20 Oct 2016 14:02:05 +0000 http://www.hindawi.com/journals/mpe/2016/3154683/ The fixed point technique has been employed in the stability analysis of time-delays bidirectional associative memory (BAM) neural networks with impulse. By formulating a contraction mapping in a product space, a new LMI-based exponential stability criterion was derived. Lately, fixed point methods have educed various good results inspiring this work, but those criteria cannot be programmed by a computer. In this paper, LMI conditions of the obtained result can be applicable to computer Matlab LMI toolbox which meets the need of the large-scale calculation in real engineering. Moreover, a numerical example and a comparable table are presented to illustrate the effectiveness of the proposed methods. Ruofeng Rao, Zhilin Pu, Shouming Zhong, and Xinggui Li Copyright © 2016 Ruofeng Rao et al. All rights reserved. The Effect of Salt-Processed Psoralea corylifolia on Generative Organ Targeting Thu, 20 Oct 2016 14:01:46 +0000 http://www.hindawi.com/journals/jamc/2016/7484202/ Psoralen and isopsoralen are two isomers and main effective components within Psoralea corylifolia. In order to investigate the salt-processing effect on tissue distribution characters of psoralen and isopsoralen, a sensitive and accurate ultrahigh pressure liquid chromatographic tandem mass spectrometric (UHPLC-MS/MS) method has been developed and validated for simultaneous determination of the 2 components in rats’ tissues after administration of the extracts that came from either crude or salt-processed Psoralea corylifolia L. Data displayed that both areas under the curve (AUC) of psoralen and isopsoralen from salt-processed scurfpea fruit group were significantly increased compared with that of the crude herb group, especially in heart (), ovary, and testes (). Though the RE and of psoralen and isopsoralen in all of the investigated organs were over 1.0, generative organs kept the maximum value. The experiment manifested that salt-processing of scurfpea fruit can increase the distribution of psoralen and isopsoralen to generative organs, heart and spleen, and the distribution of psoralen and isopsoralen to generative organs is significantly higher compared to heart and spleen (). Results indicate that salt-processing of scurfpea fruit can significantly increase the distribution of psoralen and isopsoralen to generative organs. Gen-hua Zhao, Cui-ping Yan, Zi-sheng Xu, Qian-qian Gao, Zhi-peng Chen, and Wei-dong Li Copyright © 2016 Gen-hua Zhao et al. All rights reserved. A Review of the Evaluation, Control, and Application Technologies for Drill String Vibrations and Shocks in Oil and Gas Well Thu, 20 Oct 2016 14:01:34 +0000 http://www.hindawi.com/journals/sv/2016/7418635/ Drill string vibrations and shocks (V&S) can limit the optimization of drilling performance, which is a key problem for trajectory optimizing, wellbore design, increasing drill tools life, rate of penetration, and intelligent drilling. The directional wells and other special trajectory drilling technologies are often used in deep water, deep well, hard rock, and brittle shale formations. In drilling these complex wells, the cost caused by V&S increases. According to past theories, indoor experiments, and field studies, the relations among ten kinds of V&S, which contain basic forms, response frequency, and amplitude, are summarized and discussed. Two evaluation methods are compared systematically, such as theoretical and measurement methods. Typical vibration measurement tools are investigated and discussed. The control technologies for drill string V&S are divided into passive control, active control, and semiactive control. Key methods for and critical equipment of three control types are compared. Based on the past development, a controlling program of drill string V&S is devised. Application technologies of the drill string V&S are discussed, such as improving the rate of penetration, controlling borehole trajectory, finding source of seismic while drilling, and reducing the friction of drill string. Related discussions and recommendations for evaluating, controlling, and applying the drill string V&S are made. Guangjian Dong and Ping Chen Copyright © 2016 Guangjian Dong and Ping Chen. All rights reserved. Assessment of Heat Hazard during the Polymerization of Selected Light-Sensitive Dental Materials Thu, 20 Oct 2016 13:59:06 +0000 http://www.hindawi.com/journals/bmri/2016/4158376/ Introduction. Polymerization of light-cured dental materials used for restoration of hard tooth tissue may lead to an increase in temperature that may have negative consequence for pulp vitality. Aim. The aim of this study was to determine maximum temperatures reached during the polymerization of selected dental materials, as well as the time that is needed for samples of sizes similar to those used in clinical practice to reach these temperatures. Materials and Methods. The study involved four composite restorative materials, one lining material and a dentine bonding agent. The polymerization was conducted with the use of a diode light-curing unit. The measurements of the external surface temperature of the samples were carried out using the Thermovision®550 thermal camera. Results. The examined materials significantly differed in terms of the maximum temperatures values they reached, as well as the time required for reaching the temperatures. A statistically significant positive correlation of the maximum temperature and the sample weight was observed. Conclusions. In clinical practice, it is crucial to bear in mind the risk of thermal damage involved in the application of light-cured materials. It can be reduced by using thin increments of composite materials. Maciej Janeczek, Katarzyna Herman, Katarzyna Fita, Krzysztof Dudek, Małgorzata Kowalczyk-Zając, Agnieszka Czajczyńska-Waszkiewicz, Dagmara Piesiak-Pańczyszyn, Piotr Kosior, and Maciej Dobrzyński Copyright © 2016 Maciej Janeczek et al. All rights reserved. Integrated Yard Space Allocation and Yard Crane Deployment Problem in Resource-Limited Container Terminals Thu, 20 Oct 2016 13:49:41 +0000 http://www.hindawi.com/journals/sp/2016/6421943/ Yard storage space and yard crane equipment are the core resources in the container terminal yard area. This paper studies the integrated yard space allocation (outbound container space) and yard crane deployment problem in resource-limited container terminals where yard space and yard cranes are extremely scarce. Two corresponding counterstrategies are introduced, respectively, and the integrated problem is solved as mixed integer programming. The model this paper formulated considers the container volume fluctuation of the service line, and the objective is a trade-off between yard sharing space and terminal operation cost. In numerical experiments, this paper tries to reveal the management meaning in practical operation of container terminal and provides decision support for terminal managers; therefore a series of scenarios are presented to analyze the relations among the yard sharing space, the number of yard cranes, the size of yard subblock, and the cost of terminal operation. Caimao Tan and Junliang He Copyright © 2016 Caimao Tan and Junliang He. All rights reserved. Development of Shear Capacity Prediction Model for FRP-RC Beam without Web Reinforcement Thu, 20 Oct 2016 13:49:28 +0000 http://www.hindawi.com/journals/amse/2016/4356967/ Available codes and models generally use partially modified shear design equation, developed earlier for steel reinforced concrete, for predicting the shear capacity of FRP-RC members. Consequently, calculated shear capacity shows under- or overestimation. Furthermore, in most models some affecting parameters of shear strength are overlooked. In this study, a new and simplified shear capacity prediction model is proposed considering all the parameters. A large database containing 157 experimental results of FRP-RC beams without shear reinforcement is assembled from the published literature. A parametric study is then performed to verify the accuracy of the proposed model. Again, a comprehensive review of 9 codes and 12 available models is done, published back from 1997 to date for comparison with the proposed model. Hence, it is observed that the proposed equation shows overall optimized performance compared to all the codes and models within the range of used experimental dataset. Md. Arman Chowdhury, Zubayer Ibna Zahid, and Md. Mashfiqul Islam Copyright © 2016 Md. Arman Chowdhury et al. All rights reserved. Uncoupling of Vascular Nitric Oxide Synthase Caused by Intermittent Hypoxia Thu, 20 Oct 2016 13:43:49 +0000 http://www.hindawi.com/journals/omcl/2016/2354870/ Objective. Obstructive sleep apnea (OSA), characterized by chronic intermittent hypoxia (CIH), is often present in diabetic (DB) patients. Both conditions are associated with endothelial dysfunction and cardiovascular disease. We hypothesized that diabetic endothelial dysfunction is further compromised by CIH. Methods. Adult male diabetic (BKS.Cg-Dock7m +/+ Leprdb/J) (db/db) mice (10 weeks old) and their heterozygote littermates were subjected to CIH or intermittent air (IA) for 8 weeks. Mice were separated into 4 groups: IA (intermittent air nondiabetic), IH (intermittent hypoxia nondiabetic), IADB (intermittent air diabetic), and IHDB (intermittent hypoxia diabetic) groups. Endothelium-dependent and endothelium-independent relaxation and modulation by basal nitric oxide (NO) were analyzed using wire myograph. Plasma 8-isoprostane, interleukin-6 (IL-6), and asymmetric dimethylarginine (ADMA) were measured using ELISA. Uncoupling of eNOS was measured using dihydroethidium (DHE) staining. Results. Endothelium-dependent vasodilation and basal NO production were significantly impaired in the IH and IADB group compared to IA group but was more pronounced in IHDB group. Levels of 8-isoprostane, IL-6, ADMA, and eNOS uncoupling were ≈2-fold higher in IH and IADB groups and were further increased in the IHDB group. Conclusion. Endothelial dysfunction is more pronounced in diabetic mice subjected to CIH compared to diabetic or CIH mice alone. Oxidative stress, ADMA, and eNOS uncoupling were exacerbated by CIH in diabetic mice. Mohammad Badran, Bisher Abuyassin, Saeid Golbidi, Najib Ayas, and Ismail Laher Copyright © 2016 Mohammad Badran et al. All rights reserved. Patient Perceptions of Open, Laparoscopic, and Robotic Gynecological Surgeries Thu, 20 Oct 2016 13:42:34 +0000 http://www.hindawi.com/journals/bmri/2016/4284093/ Objective. To investigate patient knowledge and attitudes toward surgical approaches in gynecology. Design. An anonymous Institutional Review Board (IRB) approved questionnaire survey. Patients/Setting. A total of 219 women seeking obstetrical and gynecological care in two offices affiliated with an academic medical center. Results. Thirty-four percent of the participants did not understand the difference between open and laparoscopic surgeries. 56% of the participants knew that laparoscopy is a better surgical approach for patients than open abdominal surgeries, while 37% thought that laparoscopy requires the surgeon to have a higher technical skill. 46% of the participants do not understand the difference between laparoscopic and robotic procedures. 67.5% of the participants did not know that the surgeon moves the robot’s arms to perform the surgery. Higher educational level and/or history of previous abdominal surgeries were associated with the highest rates of answering all the questions correctly (), after controlling for age and race. Conclusions. A substantial percentage of patients do not understand the difference between various surgical approaches. Health care providers should not assume that their patients have an adequate understanding of their surgical options and accordingly should educate them about those options so they can make truly informed decisions. Mohamad Irani, Cheruba Prabakar, Sepide Nematian, Nitasha Julka, Devika Bhatt, and Pedram Bral Copyright © 2016 Mohamad Irani et al. All rights reserved. Management of Respiratory Syncytial Virus Bronchiolitis: 2015 Survey of Members of the European Society for Paediatric Infectious Diseases Thu, 20 Oct 2016 13:38:32 +0000 http://www.hindawi.com/journals/cjidmm/2016/9139537/ In 1995, the European Society for Paediatric Infectious Diseases (ESPID) carried out a survey of its members to assess the variation in management of respiratory syncytial virus (RSV) bronchiolitis. The aim of the current study was to carry out a similar survey 20 years later to assess how the management had changed. An electronic, structured, English language survey, based on the United Kingdom National Institute for Health and Care Excellence (NICE) bronchiolitis draft guideline, was sent to ESPID members in March 2015. Questions asked included information on treatment practices of infants with bronchiolitis and doctor demographics. We received responses from 135 doctors (14% of the ESPID members) who worked in 115 hospitals. 56% of the doctors used a written guideline to manage bronchiolitic infants. All doctors stated that they isolated individually or in cohorts all hospitalised bronchiolitis infants. The level of oxygen saturation suggested as an indication to administer supplemental oxygen varied between <89% and <95%. We found significant reductions in the use of ribavirin, bronchodilators, and corticosteroids from 1995 to 2015 (ribavirin 57% to 13%, ; bronchodilators 95% to 82%, ; corticosteroids 81% to 45%, ). Although variability in management remains high, encouragingly significantly fewer doctors are prescribing ribavirin, bronchodilators, and corticosteroids compared to 20 years ago. Elliott J. Carande, Andrew J. Pollard, and Simon B. Drysdale Copyright © 2016 Elliott J. Carande et al. All rights reserved. Perceptions and Practices regarding Breastfeeding among Postnatal Women at a District Tertiary Referral Government Hospital in Southern India Thu, 20 Oct 2016 13:32:44 +0000 http://www.hindawi.com/journals/scientifica/2016/5430164/ Background. Breastfeeding is the optimal method for achieving a normal growth and development of the baby. This study aimed to study mothers’ perceptions and practices regarding breastfeeding in Mangalore, India. Methodology. A cross-sectional study of 188 mothers was conducted using a structured proforma. Results. Importance of breast feeding was known to most mothers. While initiation of breast feeding within one hour of birth was done by majority of mothers, few had discarded colostrum and adopted prelacteal feeding. Mothers opined that breast feeding is healthy for their babies (96.3%) and easier than infant feeding (79.8%), does not affect marital relationship (51%), and decreases family expenditure (61.1%). However, there were poor perceptions regarding the advantages of breast milk with respect to nutritive value, immune effect, and disease protection. Few respondents reported discontinuation of breastfeeding in previous child if the baby had fever/cold (6%) or diarrhea (18%) and vomiting (26%). There was a statistically significant association between mother’s educational level and perceived importance of breastfeeding and also between the mode of delivery and initiation of breast feeding (). Conclusion. Importance of breast feeding was known to most mothers. Few perceptions related to breast milk and feeding along with myths and disbeliefs should be rectified by health education. Sowmini P. Kamath, Dikshy Garg, Mohd. Khursheed Khan, Animesh Jain, and B. Shantharam Baliga Copyright © 2016 Sowmini P. Kamath et al. All rights reserved. Pharmacological Relaxation of the Ureter When Using Ureteral Access Sheaths during Ureterorenoscopy: A Randomized Feasibility Study in a Porcine Model Thu, 20 Oct 2016 13:27:14 +0000 http://www.hindawi.com/journals/au/2016/8064648/ Objective. High intraluminal pressure during ureterorenoscopy (URS) increases risk of infectious and haemorrhagic complications. Intrarenal pressure may be reduced by the use of ureteral access sheaths (UASs), which on the other hand may cause ureteral damage. We have previously shown that the β-agonist isoproterenol (ISO), when administered topically in the irrigation fluid, is able to inhibit ureteral muscle tone and lower intrarenal pressure during URS. The aim of this study was to examine the effect of ISO on the success rate of UAS insertion in a porcine model. Materials and Methods. 22 pigs in which a UAS could not initially be placed were randomized to endoluminal irrigation with either ISO (0.1 μg/mL) or saline before a new insertion trial. Subsequently, it was registered whether the UAS could be passed without resistance. During extraction of the sheath, any ureteral lesions were characterized ureteroscopically using the PULS classification system. Surgeons were blinded to randomization. Results. In the ISO group, the observed effect of irrigation was 63% successful UAS insertions, compared to 27% in the saline group. No serious lesions (<PULS grade 2) were observed in the ISO group. Conclusions. Endoluminal irrigation with ISO may facilitate UAS insertion and potentially decrease UAS related ureteral lesions. Søren Kissow Lildal, Kim Hovgaard Andreassen, Frederikke Eichner Christiansen, Helene Jung, Malene Roland Pedersen, and Palle Jörn Sloth Osther Copyright © 2016 Søren Kissow Lildal et al. All rights reserved. Hydrogen Sulfide Regulates the [Ca2+]i Level in the Primary Medullary Neurons Thu, 20 Oct 2016 13:14:38 +0000 http://www.hindawi.com/journals/omcl/2016/2735347/ In the present study, we attempted to elucidate mechanisms for the regulation of intracellular calcium levels by H2S in primary rat medullary neurons. Our results showed that NaHS significantly increased the level of in rat medullary neurons in a concentration-dependent manner. L-Cysteine and SAM significantly raised the level of in the medullary neurons while HA and/or AOAA produced a reversal effect. In addition, L-cysteine and SAM significantly increased but HA and/or AOAA decreased the production of H2S in the cultured neurons. The elevation induced by H2S was significantly diminished by EGTA-Ca2+-free solutions, and this elevation was also reduced by nifedipine or nimodipine and mibefradil, suggesting the role of L-type and/or T-type Ca2+ channels. Moreover, the effect of H2S on level in neurons was significantly attenuated by BAPTA-AM and thapsigargin, suggesting the source of Ca2+. Therefore, we concluded that both exogenous and endogenous H2S elevates level in primarily cultured rat medullary neurons via both increasing calcium influx and mobilizing intracellular Ca2+ stores from ER. Xiaoni Liu, Nana Zhang, Yingjiong Ding, Dongqing Cao, Ying Huang, Xiangjun Chen, Rui Wang, and Ning Lu Copyright © 2016 Xiaoni Liu et al. All rights reserved. Chronic Leptomeningitis and Spinal Intradural Mass Secondary to Alternaria Infection in a Patient with Ventriculoperitoneal Shunt Thu, 20 Oct 2016 13:10:01 +0000 http://www.hindawi.com/journals/criid/2016/4693409/ Fungal infection following placement of ventriculostomy or ventriculoperitoneal (VP) shunt is uncommon. We report the first case of Alternaria related central nervous system (CNS) shunt infection in a patient with CNS ependymoma manifesting as leptomeningitis and a spinal intradural mass. This case illustrates the diagnostic and management challenges. Sanjeet S. Dadwal, Russell Thompson, Rahul Jandial, Bernard Tegtmeier, and Mike Yue Chen Copyright © 2016 Sanjeet S. Dadwal et al. All rights reserved. Primary Neuroendocrine Carcinoma of the Breast: Histopathological Criteria, Prognostic Factors, and Review of the Literature Thu, 20 Oct 2016 13:05:48 +0000 http://www.hindawi.com/journals/cripa/2016/6762085/ We present here a case of a 42-year-old woman diagnosed with primary neuroendocrine carcinoma of the breast (NECB). We discuss the importance of histological criteria for primary neuroendocrine mammary carcinoma, established by WHO in 2003 and 2012. After an overview of different cases of primary neuroendocrine carcinoma of the breast published in the literature, we present information about differential diagnosis, prognostic factors, and surgical and adjuvant treatment. Prognosis of NECB is not different from that of other invasive breast carcinomas and the most important prognostic factor is tumor grade (G). There is no standard treatment and patients should be treated similarly to patients with invasive ductal carcinoma, NOS (not otherwise specified), whose choice of therapy depends on tumor’s size, degree of differentiation, clinical stage, and hormonal status. Lena Marinova, Doroteya Malinova, and Snezhinka Vicheva Copyright © 2016 Lena Marinova et al. All rights reserved. Chemically Diverse Secondary Metabolites from Davidia involucrata (Dove Tree) Thu, 20 Oct 2016 13:03:13 +0000 http://www.hindawi.com/journals/jchem/2016/9806102/ Dove tree, Davidia involucrata Baill. which is endemic in western China, is not only one of the best known relict species of the tertiary, but also a famous ornamental plant with dove-shaped flowers. A diverse array of secondary metabolites have been isolated and identified from Davidia involucrata. The 58 structures of the secondary metabolites were presented and classified as triterpenoids, tannins, phenolic compounds, lignans, flavonoids, alkaloids, and sterol. In addition, the biosynthetic route of some triterpenoids was proposed. Moreover, the advances in the discovery of unprecedented compounds and uncovering of notable bioactivities were highlighted in this review. Li-Yan Song, Ran Lin, Zu-Jian Wu, and Ming-An Ouyang Copyright © 2016 Li-Yan Song et al. All rights reserved. A Rare Primary Neuroendocrine Tumor (Typical Carcinoid) of the Sublingual Gland Thu, 20 Oct 2016 13:00:33 +0000 http://www.hindawi.com/journals/crid/2016/7462690/ A typical carcinoid is extremely rare in the oral cavity. We here present a case of a typical carcinoid arising in the sublingual gland of a 62-year-old woman. The tumor was removed by primary excision with 10 mm surgical margins and submandibular dissection. Examination of the tumor showed medium-sized tumor cells that were positive for CD56 and chromogranin A, with no necrosis, and with a mitotic count less than 1/10 HPF. A pathological diagnosis of typical carcinoid was made from both morphological and immunological examinations. One year after excision surgery, there was no tumor recurrence or neck metastasis. Kenji Yamagata, Kousuke Ohki, Fumihiko Uchida, Naomi Kanno, Shogo Hasegawa, Toru Yanagawa, and Hiroki Bukawa Copyright © 2016 Kenji Yamagata et al. All rights reserved. Research and Application of Influences of Lateral Pressure Coefficients on the Extension Angle of Coal Cracks Thu, 20 Oct 2016 13:00:07 +0000 http://www.hindawi.com/journals/mpe/2016/3068347/ Fluid-solid coupling seepage fields are ubiquitous in engineering practices. However, few investigations have been carried out on the rules of crack extension of solids under the effect of fluids. By using the RFPA2D (realistic failure process analysis in 2 dimensions), this research studied the influences of different lateral pressure coefficients on the extension direction and length of coal cracks. Also the result can be proved by fracture mechanics, stress intensity factor theory, and sliding crack theory. On this basis, aiming at a coal mine with the mining depth being over 700 m, the reliability of the above conclusion was further proved by testing the crustal stress at the positions with the buried depth varying from 750 m to 1,300 m. At the same time, in condition of meeting the water pressure required by the crack extension, permeability-increasing radius is selected preferably through AE energy index by simulation of hydraulic fracturing for coal seams under different lateral pressure coefficients, and the gas drainage achieves good effect in engineering practice. Cheng Liu, Shu-gang Li, Song Qin, and Shou-guo Yang Copyright © 2016 Cheng Liu et al. All rights reserved. Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke Thu, 20 Oct 2016 12:58:15 +0000 http://www.hindawi.com/journals/sci/2016/6104780/ Currently, there is increasing interest in human bone marrow stromal cells (hBMSCs) as regeneration therapy against cerebral stroke. The aim of the present study was to evaluate the feasibility and validity of hBMSC cultures with allogeneic platelet lysates (PLs). Platelet concentrates (PC) were harvested from healthy volunteers and made into single donor-derived PL (sPL). The PL mixtures (mPL) were made from three different sPL. Some growth factors and platelet cell surface antigens were detected by enzyme-linked immunosorbent assay (ELISA). The hBMSCs cultured with 10% PL were analyzed for their proliferative potential, surface markers, and karyotypes. The cells were incubated with superparamagnetic iron oxide (SPIO) agents and injected into a pig brain. MRI and histological analysis were performed. Consequently, nine lots of sPL and three mPL were prepared. ELISA analysis showed that PL contained adequate growth factors and a particle of platelet surface antigens. Cell proliferation capacity of PLs was equivalent to or higher than that of fetal calf serum (FCS). No contradiction in cell surface markers and no chromosomal aberrations were found. The MRI detected the distribution of SPIO-labeled hBMSCs in the pig brain. In summary, the hBMSCs cultured with allogeneic PL are suitable for cell therapy against stroke. Chengbo Tan, Hideo Shichinohe, Zifeng Wang, Shuji Hamauchi, Takeo Abumiya, Naoki Nakayama, Ken Kazumata, Tsuneo Ito, Kohsuke Kudo, Shigeru Takamoto, and Kiyohiro Houkin Copyright © 2016 Chengbo Tan et al. All rights reserved. Usefulness of Low-Dose Splenic Irradiation prior to Reduced-Intensity Conditioning Regimen for Hematopoietic Stem Cell Transplantation in Elderly Patients with Myelofibrosis Thu, 20 Oct 2016 12:56:17 +0000 http://www.hindawi.com/journals/crihem/2016/8751329/ The Janus kinase (JAK) 1 and 2 inhibitor, ruxolitinib, was recently approved in Japan and has been effective in many patients with myelofibrosis (MF). Although the inhibitor decreases splenomegaly and relieves MF-related symptoms, allogeneic hematopoietic cell transplantation (HCT) remains as the only curative therapy for MF. The presence of splenomegaly has been reported as a risk factor for graft failure, delayed engraftment, and poor survival. Here, we report two elderly MF patients with massive splenomegaly and a JAK2 V617F mutation. These patients underwent splenic irradiation to decrease splenomegaly prior to HCT with a reduced-intensity conditioning (RIC) regimen. Massive splenomegaly gradually decreased by 4 Gy splenic irradiation. The subsequent RIC regimen involved 4 Gy total body irradiation and fludarabine and intravenous busulfan. In both patients, engraftment failure did not occur, and complete remission was achieved. The splenomegaly decreased, and MF-related symptoms were resolved. Furthermore, the JAK2 V617F mutation disappeared, and fibrosis in the bone marrow regressed. We suggest that splenic irradiation prior to the RIC regimen for HCT in elderly MF patients with massive splenomegaly is safe. Furthermore, the HCT protocols with splenic irradiation should be considered for patients who have not shown clinical benefits to optimal medical management such as treatment with ruxolitinib. Etsuko Matsubara, Jun Yamanouchi, Riko Kitazawa, Taichi Azuma, Hiroshi Fujiwara, Takaaki Hato, and Masaki Yasukawa Copyright © 2016 Etsuko Matsubara et al. All rights reserved. Gender-Specific Hippocampal Dysrhythmia and Aberrant Hippocampal and Cortical Excitability in the APPswePS1dE9 Model of Alzheimer’s Disease Thu, 20 Oct 2016 12:54:31 +0000 http://www.hindawi.com/journals/np/2016/7167358/ Alzheimer’s disease (AD) is a multifactorial disorder leading to progressive memory loss and eventually death. In this study an APPswePS1dE9 AD mouse model has been analyzed using implantable video-EEG radiotelemetry to perform long-term EEG recordings from the primary motor cortex M1 and the hippocampal CA1 region in both genders. Besides motor activity, EEG recordings were analyzed for electroencephalographic seizure activity and frequency characteristics using a Fast Fourier Transformation (FFT) based approach. Automatic seizure detection revealed severe electroencephalographic seizure activity in both M1 and CA1 deflection in APPswePS1dE9 mice with gender-specific characteristics. Frequency analysis of both surface and deep EEG recordings elicited complex age, gender, and activity dependent alterations in the theta and gamma range. Females displayed an antithetic decrease in theta (θ) and increase in gamma (γ) power at 18-19 weeks of age whereas related changes in males occurred earlier at 14 weeks of age. In females, theta (θ) and gamma (γ) power alterations predominated in the inactive state suggesting a reduction in atropine-sensitive type II theta in APPswePS1dE9 animals. Gender-specific central dysrhythmia and network alterations in APPswePS1dE9 point to a functional role in behavioral and cognitive deficits and might serve as early biomarkers for AD in the future. Anna Papazoglou, Julien Soos, Andreas Lundt, Carola Wormuth, Varun Raj Ginde, Ralf Müller, Christina Henseler, Karl Broich, Kan Xie, Dan Ehninger, Britta Haenisch, and Marco Weiergräber Copyright © 2016 Anna Papazoglou et al. All rights reserved. Effect of Sulindac Binary System on In Vitro and In Vivo Release Profiles: An Assessment of Polymer Type and Its Ratio Thu, 20 Oct 2016 12:50:01 +0000 http://www.hindawi.com/journals/bmri/2016/3182358/ The bioavailability of sulindac (SDC), a nonsteroidal anti-inflammatory drug, is low due to poor aqueous solubility and poor dissolution rate. For this reason it is necessary to enhance the solubility and enhance dissolution of the drug by dispersing SDC in polyethylene glycols 6000 (PEG 6000) and polyvinyl pyrrolidone 40000 (PVP 40000) matrices using the coevaporation technique. Studying the influence of SDC to polymer ratio on drug content, percent yield, particle size, and in vitro release was performed. Differential scanning calorimetry, X-ray diffraction, and scanning electron microscopy were used to characterize any change in crystal habit of SDC in the prepared formulae. The anti-inflammatory effect of SDC was studied using the hind paw edema model. It was found that incorporation of SDC in PEG 6000 and PVP 40000 matrices resulted in improving the dissolution rate, which was found to depend on the polymer and its weight ratio of the drug. It is clearly obvious that the dissolution rate was remarkably improved in drug PVP 40000 molecular dispersions when compared to drug PEG 6000 systems. Solid dispersion of SDC in PEG and PVP improved the anti-inflammatory effect of SDC and it was found that formula SDV5 exhibited a more pronounced inhibition of swelling than other formulae. Gamal A. Shazly Copyright © 2016 Gamal A. Shazly. All rights reserved. Notes on the Wild Tomistoma Populations in Western Sarawak, Malaysian Borneo Thu, 20 Oct 2016 12:12:02 +0000 http://www.hindawi.com/journals/ijecol/2016/4357623/ Tomistoma schlegelii, the Freshwater Malayan Gharial or Tomistoma (local name: buaya jejulong), is secretive in nature; thus, very limited information is currently available on its wild population, especially in Sarawak. The objective of this study is to describe effort in assessing the Tomistoma wild populations in Ulu Sebuyau, Samarahan, and Serian, western Sarawak. Despite many challenges faced, this research has been successful at different levels. There was no Tomistoma seen during day surveys (2014-2015) in tributary of Samarahan River (Samarahan), Bunga-Baki River (Serian), and Kepayang River (Ulu Sebuyau). However, the research team came across two incidents of Tomistoma captured by the local people: (i) an adult male Tomistoma, captured in February 2015 in peat swamp area of Samarahan River tributary, and (ii) a hatchling, caught in Bunga-Baki River in March 2015. The sightings of Tomistoma in Kepayang River were reported by local people in February 2016; however, no individual was captured. The presence of hatchling in Bunga-Baki River indicates that a breeding population is most likely to occur in the area, and this gives hope for future conservation of Tomistoma in Sarawak. All sampling sites recorded almost similar water quality parameters and landscape of peat swamp areas, which previous studies claimed to be the potential Tomistoma habitats. The findings of this study should help relevant state agencies to step up efforts related to conservation of Tomistoma in Sarawak. Ruhana Hassan, Rambli Ahmad, Muhammad Amirul Arib Md Adzhar, Mohd Izwan Zulaini Abdul Gani, Anuar Ayob, and Ramlah Zainudin Copyright © 2016 Ruhana Hassan et al. All rights reserved. Optical Coherence Tomography as a Biomarker for Diagnosis, Progression, and Prognosis of Neurodegenerative Diseases Thu, 20 Oct 2016 12:06:13 +0000 http://www.hindawi.com/journals/joph/2016/8503859/ Neurodegenerative diseases present a current challenge for accurate diagnosis and for providing precise prognostic information. Developing imaging biomarkers for multiple sclerosis (MS), Parkinson disease (PD), and Alzheimer’s disease (AD) will improve the clinical management of these patients and may be useful for monitoring treatment effectiveness. Recent research using optical coherence tomography (OCT) has demonstrated that parameters provided by this technology may be used as potential biomarkers for MS, PD, and AD. Retinal thinning has been observed in these patients and new segmentation software for the analysis of the different retinal layers may provide accurate information on disease progression and prognosis. In this review we analyze the application of retinal evaluation using OCT technology to provide better understanding of the possible role of the retinal layers thickness as biomarker for the detection of these neurodegenerative pathologies. Current OCT analysis of the retinal nerve fiber layer and, specially, the ganglion cell layer thickness may be considered as a good biomarker for disease diagnosis, severity, and progression. Maria Satue, Javier Obis, Maria J. Rodrigo, Sofia Otin, Maria I. Fuertes, Elisa Vilades, Hector Gracia, Jose R. Ara, Raquel Alarcia, Vicente Polo, Jose M. Larrosa, Luis E. Pablo, and Elena Garcia-Martin Copyright © 2016 Maria Satue et al. All rights reserved. Community Perception towards Mental Illness among Residents of Gimbi Town, Western Ethiopia Thu, 20 Oct 2016 12:06:11 +0000 http://www.hindawi.com/journals/psychiatry/2016/6740346/ Background. Despite the increased burden of mental health problem, little is known about knowledge and perception of the public towards mental health problems in Ethiopia. Methods. Community based cross-sectional study was conducted among selected 845 Gimbi town residents from May 28 to June 28, 2014. Results. Out of the total study participants, 304 (37.3%) were found to have poor perception (a score below mean five semantic differential scales for positive questions and above mean for negative questions) of mental illness. Being above 28 years of age (AOR = 0.48 CI (0.23, 0.78)), private workers (AOR = 0.41 CI (0.19, 0.87)), and lack of mental health information were found to be associated with poor perception of mental illness (AOR = 0.133 CI (0.09, 0.20)). Absence of family history of mental illness was also found to be associated with poor perception of mental illness (AOR = 0.37 CI (0.21, 0.66)). Conclusions. Significant proportions of the community in Gimbi town were found to have poor perception of mental illness. Poor perception is common among old aged, less educated, private workers, those unable to access mental health information, and those with no family history of mental illness. Mental health education on possible causes, treatment options, and possible outcome of treatment to the community is required. Misael Benti, Jemal Ebrahim, Tadesse Awoke, Zegeye Yohannis, and Asres Bedaso Copyright © 2016 Misael Benti et al. All rights reserved. Urinary Concentrations of Steroids in Bulls under Anabolic Treatment by Revalor-XS® Implant Thu, 20 Oct 2016 12:05:41 +0000 http://www.hindawi.com/journals/jamc/2016/8013175/ Despite the European ban of using anabolics in food-producing animals, growth promoters might still be illegally used in the European Union. To control the food chain and guarantee consumers’ health, there is a need of highly sensitive analytical methods for the identification of marker residues of such treatments. In the present study, a group of bulls () received trenbolone acetate (200 mg) and estradiol (40 mg) by a commercial ear implant during a time range of 71 days, and a second group () was kept for control. The aim of the research was to measure the residual urinary concentrations of the administered drugs (β-trenbolone and β-estradiol), their main metabolites (α-trenbolone and α-estradiol), and possible alterations of the urinary profile of other endogenous hormones metabolically related. The analytical method was based on liquid chromatography-tandem mass spectrometry. Results showed average urinary concentrations of α-trenbolone and α-estradiol during treatment in the range of () ng mL−1 and () ng mL−1, respectively, whereas β-trenbolone and β-estradiol exhibit urinary concentrations lower than 0.22 ng mL−1 in both cases. Data obtained from the urinary profiles of endogenous steroids indicate that they could be useful to indirectly detect the ongoing treatment. Giancarlo Biancotto, Roberto Stella, Federica Barrucci, Francesca Lega, and Roberto Angeletti Copyright © 2016 Giancarlo Biancotto et al. All rights reserved. Serum Metabolomic Response to Long-Term Supplementation with all-rac-α-Tocopheryl Acetate in a Randomized Controlled Trial Thu, 20 Oct 2016 11:55:10 +0000 http://www.hindawi.com/journals/jnme/2016/6158436/ Background. The Alpha-Tocopherol, Beta-Carotene Cancer Prevention (ATBC) Study, a randomized controlled cancer prevention trial, showed a 32% reduction in prostate cancer incidence in response to vitamin E supplementation. Two other trials were not confirmatory, however. Objective. We compared the change in serum metabolome of the ATBC Study participants randomized to receive vitamin E to those who were not by randomly selecting 50 men from each of the intervention groups (50 mg/day all-rac-α-tocopheryl acetate (ATA), 20 mg/day -carotene, both, placebo). Methods. Metabolomic profiling was conducted on baseline and follow-up fasting serum (Metabolon, Inc.). Results. After correction for multiple comparisons, five metabolites were statistically significantly altered (β is the change in metabolite level expressed as number of standard deviations on the log scale): α-CEHC sulfate (, ), α-CEHC glucuronide (, ), α-tocopherol (, ), γ-tocopherol (, ), and β-tocopherol (, ). Glutarylcarnitine, beta-alanine, ornithine, and N6-acetyllysine were also decreased by ATA supplementation (β range 0.40 to −0.36), but not statistically significantly. Conclusions. Comparison of the observed metabolite alterations resulting from ATA supplementation to those in other vitamin E trials of different populations, dosages, or formulations may shed light on the apparently discordant vitamin E-prostate cancer risk findings. Alison M. Mondul, Steven C. Moore, Stephanie J. Weinstein, Anne M. Evans, Edward D. Karoly, Satu Männistö, Joshua N. Sampson, and Demetrius Albanes Copyright © 2016 Alison M. Mondul et al. All rights reserved. The Role of H. pylori CagA in Regulating Hormones of Functional Dyspepsia Patients Thu, 20 Oct 2016 11:43:32 +0000 http://www.hindawi.com/journals/grp/2016/7150959/ Helicobacter pylori (H. pylori, Hp) colonizes the stomachs of approximately 20%–80% of humans throughout the world. The Word Healthy Organization (WHO) classified H. pylori as a group 1 carcinogenic factor in 1994. Recently, an increasing number of studies has shown an association between H. pylori infection and various extragastric diseases. Functional dyspepsia (FD) is considered a biopsychosocial disorder with multifactorial pathogenesis, and studies have shown that infection with CagA-positive H. pylori strains could explain some of the symptoms of functional dyspepsia. Moreover, CagA-positive H. pylori strains have been shown to affect the secretion of several hormones, including 5-HT, ghrelin, dopamine, and gastrin, and altered levels of these hormones might be the cause of the psychological disorders of functional dyspepsia patients. This review describes the mutual effects of H. pylori and hormones in functional dyspepsia and provides new insight into the pathogenesis of functional dyspepsia. Wang-Ping Meng, Zhong-Qiong Wang, Jia-Qi Deng, Yi Liu, Ming-Ming Deng, and Mu-Han Lü Copyright © 2016 Wang-Ping Meng et al. All rights reserved. Microbiological Safety of Kitchen Sponges Used in Food Establishments Thu, 20 Oct 2016 11:43:26 +0000 http://www.hindawi.com/journals/ijfs/2016/1659784/ Kitchen sponges are among the possible sources of contaminants in food establishments. The main purpose of the current study was, therefore, to assess the microbiological safety of sponges as it has been used in selected food establishments of Jimma town. Accordingly, the microbiological safety of a total of 201 kitchen sponges randomly collected from food establishments was evaluated against the total counts of aerobic mesophilic bacteria (AMB), Enterobacteriaceae, coliforms, and yeast and molds. The mean counts of aerobic mesophilic bacteria ranged from 7.43 to 12.44 log CFU/mm3. The isolated genera were dominated by Pseudomonas (16.9%), Bacillus (11.1%), Micrococcus (10.6%), Streptococcus (7.8%), and Lactobacillus (6%) excluding the unidentified Gram positive rods (4.9%) and Gram negative rods (9.9%). The high microbial counts (aerobic mesophilic bacteria, coliforms, Enterobacteriaceae, and yeast and molds) reveal the existence of poor kitchen sponge sanitization practice. Awareness creation training on basic hygienic practices to food handlers and periodic change of kitchen sponges are recommended. Tesfaye Wolde and Ketema Bacha Copyright © 2016 Tesfaye Wolde and Ketema Bacha. All rights reserved. Ipsilesional High Frequency Repetitive Transcranial Magnetic Stimulation Add-On Therapy Improved Diffusion Parameters of Stroke Patients with Motor Dysfunction: A Preliminary DTI Study Thu, 20 Oct 2016 11:19:10 +0000 http://www.hindawi.com/journals/np/2016/6238575/ Purpose. The aim of this study was to evaluate the effects of high frequency repetitive transcranial magnetic stimulation (HF-rTMS) on stroke patients with motor dysfunction and to investigate the underlying neural mechanism. Methods. Fifteen stroke patients were assigned to the rTMS treatment (RT) group and conventional treatment (CT) group. Patients in the RT received 10 Hz rTMS stimulation on the ipsilesional primary motor cortex for 10 days plus conventional treatment of CT, which consisted of acupuncture and antiplatelet aggregation medication. Difference in fractional anisotropy (FA) between pretreatment and posttreatment and between two groups was determined. Correlations between FA values and neurological assessments were also calculated. Results. Both groups significantly improved the neurological function after treatment. rTMS-treated patients showed better improvement in Fugl-Meyer Assessment (FMA) score and increased FA value in motor-related white matter and gray matter cortices compared with CT-treated patients and pretreatment status. Besides, the increased FA value in the ipsilesional posterior limb of the internal capsule in RT group was significantly correlated with the improved FMA score. Significance. HF-rTMS could be a supplement therapy to CT in improving motor recovery in patients with stroke. And this benefit effect may be achieved through modulating the ipsilesional corticospinal tracts and motor-related gray matter cortices. Zhiwei Guo, Yu Jin, Haitao Peng, Guoqiang Xing, Xiang Liao, Yunfeng Wang, Huaping Chen, Bin He, Morgan A. McClure, and Qiwen Mu Copyright © 2016 Zhiwei Guo et al. All rights reserved. Role of Nitric Oxide, Nitric Oxide Synthase, Soluble Guanylyl Cyclase, and cGMP-Dependent Protein Kinase I in Mouse Stem Cell Cardiac Development Thu, 20 Oct 2016 11:13:35 +0000 http://www.hindawi.com/journals/sci/2016/2868323/ Introduction and Aim. Nitric oxide (NO) can trigger cardiac differentiation of embryonic stem cells (ESCs), indicating a cardiogenic function of the NO synthetizing enzyme(s) (NOS). However, the involvement of the NO/NOS downstream effectors soluble guanylyl cyclase (sGC) and cGMP activated protein kinase I (PKG-I) is less defined. Therefore, we assess the involvement of the entire NO/NOS/sGC/PKG-I pathway during cardiac differentiation process. Methods. Mouse ESCs were differentiated toward cardiac lineages by hanging drop methodology for 21 days. NOS/sGC/PKG-I pathway was studied quantifying genes, proteins, enzymatic activities, and effects of inhibition during differentiation. Percentages of beating embryoid bodies (mEBs) were evaluated as an index of cardiogenesis. Results and Discussion. Genes and protein expression of enzymes were increased during differentiation with distinctive kinetics and proteins possessed their enzymatic functions. Exogenous administered NO accelerated whereas the blockade of PKG-I strongly slowed cardiogenesis. sGC inhibition was effective only at early stages and NOS blockade ineffective. Of NOS/sGC/PKG-I pathway, PKG-I seems to play the prominent role in cardiac maturation. Conclusion. We concluded that exogenous administered NO and other pharmacological strategies able to increase the activity of PKG-I provide new tools to investigate and promote differentiation of cardiogenic precursors. Valentina Spinelli, Alessia Vona, Francesca Corti, Lorenzo Diolaiuti, Matteo Zanardelli, Laura Sartiani, and Paola Failli Copyright © 2016 Valentina Spinelli et al. All rights reserved. Microwave-Assisted Tissue Preparation for Rapid Fixation, Decalcification, Antigen Retrieval, Cryosectioning, and Immunostaining Thu, 20 Oct 2016 11:10:42 +0000 http://www.hindawi.com/journals/ijcb/2016/7076910/ Microwave irradiation of tissue during fixation and subsequent histochemical staining procedures significantly reduces the time required for incubation in fixation and staining solutions. Minimizing the incubation time in fixative reduces disruption of tissue morphology, and reducing the incubation time in staining solution or antibody solution decreases nonspecific labeling. Reduction of incubation time in staining solution also decreases the level of background noise. Microwave-assisted tissue preparation is applicable for tissue fixation, decalcification of bone tissues, treatment of adipose tissues, antigen retrieval, and other special staining of tissues. Microwave-assisted tissue fixation and staining are useful tools for histological analyses. This review describes the protocols using microwave irradiation for several essential procedures in histochemical studies, and these techniques are applicable to other protocols for tissue fixation and immunostaining in the field of cell biology. Kazuo Katoh Copyright © 2016 Kazuo Katoh. All rights reserved. The Role of Parvalbumin, Sarcoplasmatic Reticulum Calcium Pump Rate, Rates of Cross-Bridge Dynamics, and Ryanodine Receptor Calcium Current on Peripheral Muscle Fatigue: A Simulation Study Thu, 20 Oct 2016 10:36:41 +0000 http://www.hindawi.com/journals/cmmm/2016/3180205/ A biophysical model of the excitation-contraction pathway, which has previously been validated for slow-twitch and fast-twitch skeletal muscles, is employed to investigate key biophysical processes leading to peripheral muscle fatigue. Special emphasis hereby is on investigating how the model’s original parameter sets can be interpolated such that realistic behaviour with respect to contraction time and fatigue progression can be obtained for a continuous distribution of the model’s parameters across the muscle units, as found for the functional properties of muscles. The parameters are divided into 5 groups describing (i) the sarcoplasmatic reticulum calcium pump rate, (ii) the cross-bridge dynamics rates, (iii) the ryanodine receptor calcium current, (iv) the rates of binding of magnesium and calcium ions to parvalbumin and corresponding dissociations, and (v) the remaining processes. The simulations reveal that the first two parameter groups are sensitive to contraction time but not fatigue, the third parameter group affects both considered properties, and the fourth parameter group is only sensitive to fatigue progression. Hence, within the scope of the underlying model, further experimental studies should investigate parvalbumin dynamics and the ryanodine receptor calcium current to enhance the understanding of peripheral muscle fatigue. Oliver Röhrle, Verena Neumann, and Thomas Heidlauf Copyright © 2016 Oliver Röhrle et al. All rights reserved. An Explanation of the Underlying Mechanisms for the In Vitro and In Vivo Antiurolithic Activity of Glechoma longituba Thu, 20 Oct 2016 10:21:15 +0000 http://www.hindawi.com/journals/omcl/2016/3134919/ Purpose. To use in vitro and in vivo models to evaluate Glechoma longituba extract to provide scientific evidence for this extract’s antiurolithic activity. Materials and Methods. Potassium citrate was used as a positive control group. Oxidative stress (OS) markers and the expression of osteopontin (OPN) and kidney injury molecule-1 (KIM-1) were measured to assess the protective effects of Glechoma longituba. Multiple urolithiasis-related biochemical parameters were evaluated in urine and serum. Kidneys were harvested for histological examination and the assessment of crystal deposits. Results. In vitro and in vivo experiments demonstrated that treatment with Glechoma longituba extract significantly decreased calcium oxalate- (CaOx-) induced OPN expression, KIM-1 expression, and OS compared with the positive control group (). Additionally, in vivo rats that received Glechoma longituba extract exhibited significantly decreased CaOx deposits and pathological alterations () compared with urolithic rats. Significantly lower levels of oxalate, creatinine, and urea and increased citrate levels were observed among rats that received Glechoma longituba () compared with urolithic rats. Conclusion. Glechoma longituba has antiurolithic effects due to its possible combined effects of increasing antioxidant levels, decreasing urinary stone-forming constituents and urolithiasis-related protein expression, and elevating urinary citrate levels. Qiang Liang, Xiaoran Li, Wangning Zhou, Yu Su, Shenbao He, Shuanglei Cheng, Jianzhong Lu, Wenjuan Cao, Yuke Yan, Xiaxia Pei, Jin Qi, Guangli Xu, and Zhongjin Yue Copyright © 2016 Qiang Liang et al. All rights reserved. A Case of Reactive Cervical Lymphadenopathy with Fat Necrosis Impinging on Adjacent Vascular Structures Thu, 20 Oct 2016 10:20:51 +0000 http://www.hindawi.com/journals/criot/2016/6019501/ A tender neck mass in adults can be a diagnostic challenge due to a wide differential diagnosis, which ranges from reactive lymphadenopathy to malignancy. In this report, we describe a case of a young female with an unusually large and tender reactive lymph node with fat necrosis. The diagnostic imaging findings alone mimicked that of scrofula and malignancy, which prompted a complete workup. Additionally, the enlarged lymph node was compressing the internal jugular vein in the setting of oral contraceptive use by the patient, raising concern for Lemierre’s syndrome or internal jugular vein thrombosis. This report shows how, in the appropriate clinical context, and especially with the involvement of adjacent respiratory or neurovascular structures, aggressive diagnostic testing can be indicated. Albert Y. Han, Jacob F. Lentz, Edward C. Kuan, Hiwot H. Araya, and Mohammad Kamgar Copyright © 2016 Albert Y. Han et al. All rights reserved. Inconceivable Hypokalemia: A Case Report of Acute Severe Barium Chloride Poisoning Thu, 20 Oct 2016 10:19:07 +0000 http://www.hindawi.com/journals/crim/2016/2743134/ Barium is a heavy divalent alkaline earth metal that has been known as a muscle poison. Barium can cause human toxicity, which may lead to significant hypokalemia and have serious consequences. This paper reports a case of unprecedented barium intoxication in which the patient, who suffered from depression, swallowed at least 3.0 g barium chloride to commit suicide. On admission, the patient presented with nausea, vomiting, stomach burning feeling, dizziness, and weakness. Emergency biochemical testing showed that the patient was suffering from severe hypokalemia (K+ 1.7 mmol/L). His electrocardiogram (ECG) prompted atrioventricular blocking, ventricular tachycardia, prolongation of PR interval, ST segment depression with U waves, and T wave inversion. Intravenous potassium supplements were given immediately to correct hypokalemia and regular monitoring of vital signs and fluid balance was arranged. After all-out rescue of our hospital personnel, the condition of the patient is currently stable and he is gradually recovering. This case exemplifies the weaknesses of the management of toxic substances and the lack of mental health education for young people. We hope to get more attention for the supervision of toxic substances and the healthy development of young people. Haibo Tao, Yanru Man, Xiaoyuan Shi, Jun Zhu, Hang Pan, Qin Qin, and Shanrong Liu Copyright © 2016 Haibo Tao et al. All rights reserved. Qualitative and Quantitative Determination of Phytochemical Contents of Indigenous Nigerian Softwoods Thu, 20 Oct 2016 10:16:10 +0000 http://www.hindawi.com/journals/njos/2016/5601327/ The phytochemical contents of some milled Nigerian softwood chips were carried out in a quest to evaluate their potentials as sources of alternative medicine as well as uses in other industrial applications. The qualitative and quantitative analysis were ascertained. Tannin was found in all the Nigerian softwoods examined with the highest quantities obtained in Sterculia oblonga (1240 mg/100 g) and Barteria nigritiana (1230 mg/100 g). Highest quantities of alkaloid were obtained in Cordia millenii (11.2%) and Sterculia oblonga (10.4%). Barteria nigritiana (14.2%) and Moringa oleifera (12.2%) recorded more flavonoid content than other individual softwoods. Saponin was more in Anogeissus leiocarpus (12.5%) and Dichrostachys cinerea (9.8%). Oxalate was found to be higher in Combretodendron macrocarpum (5.84 g/100 g) and Glyphaea brevis (3.55 g/100 g). Pentaclethra macrophylla (890 mg/100 g) and Moringa oleifera (880 mg/100 g) contained more cyanogenic glycosides. Sacoglottis gabonensis (4.68 mg/g) and Pentaclethra macrophylla (4.04 mg/g) showed the highest contents of phenol, while more lipids (8% and 7.2%) were found in Anogeissus leiocarpus and Kaempferia galanga, respectively. The results showed that these Nigerian softwoods grains could be a source for the exploitation of these phytochemicals beneficial in the pharmaceutical and alternative medicine industries. Chukwuma S. Ezeonu and Chigozie M. Ejikeme Copyright © 2016 Chukwuma S. Ezeonu and Chigozie M. Ejikeme. All rights reserved. Successful Ablation for Atrial Tachycardia Originated from Sinus Venosa with Tachycardia-Induced Cardiomyopathy Thu, 20 Oct 2016 10:07:01 +0000 http://www.hindawi.com/journals/cric/2016/4865034/ A 74-year-old male suffering from congestive heart failure with atrial tachycardia (AT) with 2 : 1 atrioventricular conduction was admitted to our hospital. After the therapy with diuretics and β-blocker, his rapid AT was still sustained. He took the catheter ablation for his AT. Postpacing interval mapping from entrainment and noncontact mapping system revealed the mechanism of his AT, originated from sinus venosa. His AT was successfully terminated and eliminated by radiofrequency catheter ablation. After the successful ablation, he has been free from any AT, and his cardiac function was also improved. Sou Takenaka, Hideaki Sato, Mikio Yuhara, and Takashi Uchiyama Copyright © 2016 Sou Takenaka et al. All rights reserved. Retrospective Case Reports of Anemic Pregnant Women Receiving Intravenous Ferric Carboxymaltose: Experience from a Tertiary Hospital in Spain Thu, 20 Oct 2016 10:05:00 +0000 http://www.hindawi.com/journals/ogi/2016/5060252/ Iron deficiency and iron deficiency anemia during pregnancy call for safe treatment options that raise maternal hemoglobin levels and counterbalance iron demand and blood volume expansion while minimizing risks for the growing fetus. This retrospective study describes experience with intravenous ferric carboxymaltose given to pregnant women in a tertiary hospital in Spain. In a 5-year period, 95 pregnant women who had pretreatment hemoglobin <10 g/dL and at least one time of ferric carboxymaltose administration during pregnancy were included. Main outcome measures were week of pregnancy at iron administration, Hb levels before and after treatment, neonatal 5-minute Apgar scores, and birth weight. The majority received one dose of ferric carboxymaltose (1000 mg iron) during advanced pregnancy (median 31 weeks; interquartile range [IQR]: 27; 37 weeks) with minor to no adverse outcomes. Overall, median Hb increased from 8.5 g/dL (8.1; 8.9 g/dL) before treatment to 11.0 g/dL (9.9; 11.7 g/dL) after treatment. Normal Apgar scores were observed in all 97 infants (median birth weights 3560 g, 3270, and 3798 g). Four women received ferric carboxymaltose in the first trimester and twenty-eight during the second trimester without adverse outcomes for mother or child. These cases add to the evidence that ferric carboxymaltose administration during pregnancy is effective and safe. Rafael Aporta Rodriguez, Mariola García Montero, Jose Pablo Lorente Aporta, Carolina Gallego Luque, Alfonso Chacón Mayor, Jose Aragón Ruiz, Virginia Torres Degayón, Claudia García Jimenez, and Guadalupe Sanchez Sanchez Copyright © 2016 Rafael Aporta Rodriguez et al. All rights reserved. Alendronate Can Improve Bone Alterations in Experimental Diabetes by Preventing Antiosteogenic, Antichondrogenic, and Proadipocytic Effects of AGEs on Bone Marrow Progenitor Cells Thu, 20 Oct 2016 10:03:11 +0000 http://www.hindawi.com/journals/bmri/2016/5891925/ Bisphosphonates such as alendronate are antiosteoporotic drugs that inhibit the activity of bone-resorbing osteoclasts and secondarily promote osteoblastic function. Diabetes increases bone-matrix-associated advanced glycation end products (AGEs) that impair bone marrow progenitor cell (BMPC) osteogenic potential and decrease bone quality. Here we investigated the in vitro effect of alendronate and/or AGEs on the osteoblastogenic, adipogenic, and chondrogenic potential of BMPC isolated from nondiabetic untreated rats. We also evaluated the in vivo effect of alendronate (administered orally to rats with insulin-deficient Diabetes) on long-bone microarchitecture and BMPC multilineage potential. In vitro, the osteogenesis (Runx2, alkaline phosphatase, type 1 collagen, and mineralization) and chondrogenesis (glycosaminoglycan production) of BMPC were both decreased by AGEs, while coincubation with alendronate prevented these effects. The adipogenesis of BMPC (PPARγ, intracellular triglycerides, and lipase) was increased by AGEs, and this was prevented by coincubation with alendronate. In vivo, experimental Diabetes (a) decreased femoral trabecular bone area, osteocyte density, and osteoclastic TRAP activity; (b) increased bone marrow adiposity; and (c) deregulated BMPC phenotypic potential (increasing adipogenesis and decreasing osteogenesis and chondrogenesis). Orally administered alendronate prevented all these Diabetes-induced effects on bone. Thus, alendronate could improve bone alterations in diabetic rats by preventing the antiosteogenic, antichondrogenic, and proadipocytic effects of AGEs on BMPC. Sara Rocío Chuguransky, Ana María Cortizo, and Antonio Desmond McCarthy Copyright © 2016 Sara Rocío Chuguransky et al. All rights reserved. Tackling Cancer Stem Cells via Inhibition of EMT Transcription Factors Thu, 20 Oct 2016 10:00:44 +0000 http://www.hindawi.com/journals/sci/2016/5285892/ Cancer stem cell (CSC) has become recognized for its role in both tumorigenesis and poor patient prognosis in recent years. Traditional therapeutics are unable to effectively eliminate this group of cells from the bulk population of cancer cells, allowing CSCs to persist posttreatment and thus propagate into secondary tumors. The therapeutic potential of eliminating CSCs, to decrease tumor relapse, has created a demand for identifying mechanisms that directly target and eliminate cancer stem cells. Molecular profiling has shown that cancer cells and tumors that exhibit the CSC phenotype also express genes associated with the epithelial-to-mesenchymal transition (EMT) feature. Ample evidence has demonstrated that upregulation of master transcription factors (TFs) accounting for the EMT process such as Snail/Slug and Twist can reprogram cancer cells from differentiated to stem-like status. Despite being appealing therapeutic targets for tackling CSCs, pharmacological approaches that directly target EMT-TFs remain impossible. In this review, we will summarize recent advances in the regulation of Snail/Slug and Twist at transcriptional, translational, and posttranslational levels and discuss the clinical implication and application for EMT blockade as a promising strategy for CSC targeting. Megan Mladinich, Diane Ruan, and Chia-Hsin Chan Copyright © 2016 Megan Mladinich et al. All rights reserved. Duplicate Vas Deferens Encountered during Inguinal Hernia Repair: A Case Report and Literature Review Thu, 20 Oct 2016 09:55:27 +0000 http://www.hindawi.com/journals/cris/2016/8324925/ Duplication of the vas deferens is a rare anomaly, defined as the presence of two distinct vasa deferentia within one spermatic cord, with only 28 cases reported worldwide since 1959. We report the case of a 63-year-old man with a duplicate vas deferens, presenting with abdominal pain from bowel obstruction secondary to incarcerated inguinal hernia. Spermatic cord dissection during hernioplasty revealed duplication of the vas deferens within the right spermatic cord. Doppler ultrasonography confirmed absence of waveforms in both vasa deferentia with arterial signal in the accompanying vessel. The hernia was repaired without complication. This report emphasizes recognition of duplicate vas deferens in avoiding iatrogenic injury and optimizing surgical outcome. Maxwell C. Breitinger, Evan H. Roszkowski, Adam J. Bauermeister, and Andrew A. Rosenthal Copyright © 2016 Maxwell C. Breitinger et al. All rights reserved. Therapeutic Endoscopy Can Be Performed Safely in an Ambulatory Surgical Center: A Multicenter, Prospective Study Thu, 20 Oct 2016 09:51:27 +0000 http://www.hindawi.com/journals/dte/2016/7168280/ Background. Even amongst experienced endoscopists, endoscopic retrograde cholangiopancreatography (ERCP) and endoscopic ultrasound with fine needle aspiration (EUS-FNA) carry a potential risk for complications. These procedures are typically performed in a hospital-based endoscopy unit with general anesthesia. Aims. The goal of our study was to evaluate the feasibility of ERCP and EUS-FNA in an ambulatory surgical center (ASC). Methods. From June to November of 2014, we prospectively enrolled consecutive subjects undergoing ERCP and/or EUS-FNA in an ASC. An anesthesiologist, who was not involved in our study group, screened all subjects prior to their scheduled procedure. In order to monitor for adverse events (AE), all subjects received a telephone call at day 1 and 30 days after procedure. Results. 375 subjects (98 inpatients and 277 from an ASC) were enrolled. In the total population, a high proportion of subjects underwent procedures for neoplasms (21 (23.3%) inpatients versus 44 (17.1%) from an ASC) and for sphincter of Oddi dysfunction (SOD) (27 (27.5%) versus 48 (17.3%)) and had the American Society for Anesthesiologists (ASA) class ≥III (75 (76.5%) versus 140 (50.5%)) and high-risk features (17 (17.3%) versus 75 (27.1%)). Overall ERCP-related AE (10 (13.2%) versus 12 (7.5%), ), pancreatitis (7 (9.2%) versus 11 (6.9%), ), and hemorrhage (3.9% versus 0.6%, ) were not different between inpatients and ASC subjects. There was also no difference between inpatients and ASC subjects’ EUS-related AE (1 (4.5%) versus 4 (3.4%), ), pancreatitis (1 (4.5%) versus 3 (2.6%), ), and hemorrhage (0% versus 1 (0.9%), ). Conclusions. ERCP and EUS can be performed in a higher risk population under the supervision of anesthesia in ASCs. Overall, the AE are equivalent between inpatients and ASC subjects. Shaffer R. S. Mok, Henry C. Ho, John P. Gaughan, and Adam B. Elfant Copyright © 2016 Shaffer R. S. Mok et al. All rights reserved. Gene Expression Profile of Colon Mucosa after Cytotoxic Insult in wt and Apc-Mutated Pirc Rats: Possible Relation to Resistance to Apoptosis during Carcinogenesis Thu, 20 Oct 2016 09:47:55 +0000 http://www.hindawi.com/journals/bmri/2016/1310342/ Apc-mutated Pirc rats, spontaneously developing intestinal tumours, are resistant to 1,2-dimethylhydrazine- (DMH-) induced colon apoptosis. To understand this phenomenon, we analyzed the expression of genotoxic stress-related genes Mgmt, Gsta1, and Gstp1 in the colon of wt and Pirc rats in basal conditions and 24 h after DMH; plasmatic oxidant/antioxidant status was also evaluated. After DMH, Mgmt expression was increased in both genotypes but significantly only in wt rats; Gsta1 expression was significantly increased in both genotypes. Gstp1 expression did not vary after DMH but was lower in Pirc rats. Moreover, for each genotype, we studied by microarray technique whole gene expression profile after DMH. By unsupervised cluster analysis, 28 genes were differentially modulated between the two genotypes. Among them were interferon-induced genes Irf7, Oas1a, Oasl2, and Isg15 and the transcription factor Taf6l, overexpressed in DMH-treated wt rats and unchanged in Pirc rats. RT-PCR confirmed their overexpression in DMH-treated wt rats and showed a slighter variation in DMH-treated Pirc rats. Taken together, despite a blunted induction of Irf7, Oas1a, and Mgmt, defective apoptosis in Pirc rats 24 h after DMH is not mirrored by major differences in gene expression compared with wt rats. Angelo Pietro Femia, Cristina Luceri, Maura Lodovici, Stefania Crucitta, and Giovanna Caderni Copyright © 2016 Angelo Pietro Femia et al. All rights reserved. -Mixing Tuple of Operators on Banach Spaces Thu, 20 Oct 2016 09:47:17 +0000 http://www.hindawi.com/journals/jfs/2016/9251672/ We consider the question: what is the appropriate formulation of Godefroy-Shapiro criterion for tuples of operators? We also introduce a new notion about tuples of operators, -mixing, which lies between mixing and weakly mixing. We also obtain a sufficient condition to ensure a tuple of operators to be -mixing. Moreover, we study some new properties of -mixing operators on several concrete Banach spaces. Wei Wang, Yonglu Shu, and Xingzhong Wang Copyright © 2016 Wei Wang et al. All rights reserved. Robust FCM Algorithm with Local and Gray Information for Image Segmentation Thu, 20 Oct 2016 09:33:41 +0000 http://www.hindawi.com/journals/afs/2016/6238295/ The FCM (fuzzy -mean) algorithm has been extended and modified in many ways in order to solve the image segmentation problem. However, almost all the extensions require the adjustment of at least one parameter that depends on the image itself. To overcome this problem and provide a robust fuzzy clustering algorithm that is fully free of the empirical parameters and noise type-independent, we propose a new factor that includes the local spatial and the gray level information. Actually, this work provides three extensions of the FCM algorithm that proved their efficiency on synthetic and real images. Hanane Barrah, Abdeljabbar Cherkaoui, and Driss Sarsri Copyright © 2016 Hanane Barrah et al. All rights reserved. Proteome Analysis of Date Palm (Phoenix dactylifera L.) under Severe Drought and Salt Stress Thu, 20 Oct 2016 09:31:31 +0000 http://www.hindawi.com/journals/ijg/2016/7840759/ Date palm cultivars differently tolerate salinity and drought stress. This study was carried out to study the response of date palm to severe salinity and drought based on leaf proteome analysis. Eighteen-month-old date palm plants were subjected to severe salt (48 g/L NaCl) and drought (82.5 g/L PEG or no irrigation) conditions for one month. Using a protein 2D electrophoresis method, 55 protein spots were analyzed using mass spectrometry. ATP synthase CF1 alpha chains were significantly upregulated under all three stress conditions. Changes in the abundance of RubisCO activase and one of the RubisCO fragments were significant in the same spots only for salt stress and drought stress with no irrigation, and oxygen-evolving enhancer protein 2 was changed in different spots. Transketolase was significantly changed only in drought stress with PEG. The expression of salt and drought stress genes of the chosen protein spots was either overexpressed or downexpressed as revealed by the high or low protein abundance, respectively. In addition, all drought tolerance genes due to no irrigation were downregulated. In conclusion, the proteome analysis of date palm under salinity and drought conditions indicated that both salinity and drought tolerance genes were differentially expressed resulting in high or low protein abundance of the chosen protein spots as a result of exposure to drought and salinity stress condition. Haddad A. El Rabey, Abdulrahman L. Al-Malki, and Khalid O. Abulnaja Copyright © 2016 Haddad A. El Rabey et al. All rights reserved. A Service-Oriented Approach to Crowdsensing for Accessible Smart Mobility Scenarios Thu, 20 Oct 2016 09:22:12 +0000 http://www.hindawi.com/journals/misy/2016/2821680/ This work presents an architecture to help designing and deploying smart mobility applications. The proposed solution builds on the experience already matured by the authors in different fields: crowdsourcing and sensing done by users to gather data related to urban barriers and facilities, computation of personalized paths for users with special needs, and integration of open data provided by bus companies to identify the actual accessibility features and estimate the real arrival time of vehicles at stops. In terms of functionality, the first “monolithic” prototype fulfilled the goal of composing the aforementioned pieces of information to support citizens with reduced mobility (users with disabilities and/or elderly people) in their urban movements. In this paper, we describe a service-oriented architecture that exploits the microservices orchestration paradigm to enable the creation of new services and to make the management of the various data sources easier and more effective. The proposed platform exposes standardized interfaces to access data, implements common services to manage metadata associated with them, such as trustworthiness and provenance, and provides an orchestration language to create complex services, naturally mapping their internal workflow to code. The manuscript demonstrates the effectiveness of the approach by means of some case studies. Silvia Mirri, Catia Prandi, Paola Salomoni, Franco Callegati, Andrea Melis, and Marco Prandini Copyright © 2016 Silvia Mirri et al. All rights reserved. Topological Entropy of One Type of Nonoriented Lorenz-Type Maps Thu, 20 Oct 2016 09:22:05 +0000 http://www.hindawi.com/journals/ddns/2016/6987471/ Constructing a Poincaré map is a method that is often used to study high-dimensional dynamical systems. In this paper, a geometric model of nonoriented Lorenz-type attractor is studied using this method, and its dynamical property is described. The topological entropy of one-dimensional nonoriented Lorenz-type maps is also computed in terms of their kneading sequences. Guo Feng Copyright © 2016 Guo Feng. All rights reserved. A Rare Case of an Irreducible Patella Dislocation Thu, 20 Oct 2016 09:05:39 +0000 http://www.hindawi.com/journals/crim/2016/3728425/ Reports of irreducible patellar dislocations are exceedingly sparse throughout the literature. Obvious radiographic or physical exam findings including fracture or inversion of the patella are often present to explain the block to reduction. Not described previously in the literature is the instance of an irreducible patella dislocation in the setting of innocuous appearing injury imaging. We present a case of a healthy thirty-two-year-old female who sustained an irreducible lateral patella dislocation while participating in a dance aerobics class. Closed means of reduction were unsuccessful, necessitating open reduction. Intraoperative findings suggest incarceration of a nondisplaced fracture and a chondral defect as the block to reduction. Following open reduction, the patient has had no further episodes of pain or instability related to the patella at one-year follow-up. Irreducible patellar dislocations are exceedingly rare injuries, where associated osseous or chondral lesions may necessitate open reduction despite innocuous appearing initial imaging. A high index of suspicion to proceed with open reduction may limit repeated attempts at closed reduction and further injury. Dimitri E. Delagrammaticas and Scott D. Cordes Copyright © 2016 Dimitri E. Delagrammaticas and Scott D. Cordes. All rights reserved. Adalimumab Induced or Provoked MS in Patient with Autoimmune Uveitis: A Case Report and Review of the Literature Thu, 20 Oct 2016 09:01:35 +0000 http://www.hindawi.com/journals/crim/2016/1423131/ Anti-tumor necrosis factor α (anti-TNF-α) agents have been widely used in the field of autoimmune diseases and have proved decisive efficacy and relative safety. Data concerning their adverse effects has been lately describing central nervous system (CNS) demyelination process at escalating basis. Case Presentation. A 23-year-old male with autoimmune uveitis and a family history of multiple sclerosis (MS) developed two neurological attacks, after Adalimumab infusion, simultaneously with several cerebral lesions on magnetic resonance imaging (MRI). Hence the diagnosis of Adalimumab induced MS was suspected. Conclusion. This case is reported to tell physicians to be cautious when using anti-TNF-α in patients with family history of MS and to reconsider the risk of MS in patients with autoimmune diseases. Rana Alnasser Alsukhni, Ziena Jriekh, and Yasmin Aboras Copyright © 2016 Rana Alnasser Alsukhni et al. All rights reserved. Digital Dentistry: New Materials and Techniques Thu, 20 Oct 2016 08:58:13 +0000 http://www.hindawi.com/journals/ijd/2016/5261247/ Francesco Mangano, Jamil A. Shibli, and Thomas Fortin Copyright © 2016 Francesco Mangano et al. All rights reserved. Intelligent Packaging Systems: Sensors and Nanosensors to Monitor Food Quality and Safety Thu, 20 Oct 2016 08:56:47 +0000 http://www.hindawi.com/journals/js/2016/4046061/ The application of nanotechnology in different areas of food packaging is an emerging field that will grow rapidly in the coming years. Advances in food safety have yielded promising results leading to the development of intelligent packaging (IP). By these containers, it is possible to monitor and provide information of the condition of food, packaging, or the environment. This article describes the role of the different concepts of intelligent packaging. It is possible that this new technology could reach enhancing food safety, improving pathogen detection time, and controlling the quality of food and packaging throughout the supply chain. Guillermo Fuertes, Ismael Soto, Raúl Carrasco, Manuel Vargas, Jorge Sabattin, and Carolina Lagos Copyright © 2016 Guillermo Fuertes et al. All rights reserved. Structural Parameter Identification of Articulated Arm Coordinate Measuring Machines Thu, 20 Oct 2016 08:47:10 +0000 http://www.hindawi.com/journals/mpe/2016/4063046/ Precise structural parameter identification of a robotic articulated arm coordinate measuring machine (AACMM) is essential for improving its measuring accuracy, particularly in robotic applications. This paper presents a constructive parameter identification approach for robotic AACMMs. We first develop a mathematical kinematic model of the AACMM based on the Denavit-Hartenberg (DH) approach established for robotic systems. This model is further calibrated and verified via the practical test data. Based on the difference between the calculated coordinates of the AACMM probe via the kinematic model and the given reference coordinates, a parameter identification approach is proposed to estimate the structural parameters in terms of the test data set. The Jacobian matrix is further analyzed to determine the solvability of the identification model. It shows that there are two coupling parameters, which can be removed in the regressor. Finally, a parameter identification algorithm taking the least-square solution of the identification model as the structural parameters by using the obtained poses data is suggested. Practical experiments based on a robotic AACMM test rig are carried out, and the results reveal the effectiveness and robustness of the proposed identification approach. Guanbin Gao, Huaishan Zhang, Xing Wu, and Yu Guo Copyright © 2016 Guanbin Gao et al. All rights reserved. Mitochondrial Disorder Aggravated by Metoprolol Thu, 20 Oct 2016 08:41:45 +0000 http://www.hindawi.com/journals/cripe/2016/7869174/ Beta-adrenergic blocking agents or beta-blockers are a class of medications used to treat cardiac arrhythmias and systemic hypertension. In therapeutic dosages, they have known adverse outcomes that can include muscular fatigue and cramping, dizziness, and dyspnea. In patients with mitochondrial disease, these effects can be amplified. Previous case reports have been published in the adult population; however, their impact in pediatric patients has not been reported. We describe a pediatric patient with a mitochondrial disorder who developed respiratory distress after metoprolol was prescribed for hypertension. As the patient improved with discontinuation of medication and no alternative etiology was found for symptoms, we surmise that administration of metoprolol aggravated his mitochondrial dysfunction, thus worsening underlying chest wall weakness. Cheryl Samuels, Mary Kay Koenig, Mariana Hernandez, Aravind Yadav, and Ricardo A. Mosquera Copyright © 2016 Cheryl Samuels et al. All rights reserved. Ruptured Ectopic Pregnancy with Contralateral Ovarian Serous Cyst Adenoma Torsion: Laparoscopic Management of Double Trouble Thu, 20 Oct 2016 08:38:14 +0000 http://www.hindawi.com/journals/criog/2016/2980941/ Adnexal torsion is responsible for 2.7% of all gynecological emergencies. Ectopic pregnancy is relatively common, occurring in 2% of all pregnancies. We report the second case of ruptured right tubal ectopic gestation with torsion of serous cystadenoma of left ovary. This was diagnosed after emergency laparoscopy done for acute abdomen. Right salpingectomy and left ovarian detorsion followed by cystectomy were done laparoscopically. Deepa Ganesh, Anirudh Rajkumar, J. S. Rajkumar, and Venkatesan Guru Copyright © 2016 Deepa Ganesh et al. All rights reserved. Bowel Ischemia from Heat Stroke: A Rare Presentation of an Uncommon Complication Thu, 20 Oct 2016 08:27:14 +0000 http://www.hindawi.com/journals/crim/2016/5217690/ A healthy 27-year-old female presented to the hospital after she collapsed an hour into her first marathon run on a hot humid day. On presentation, she was hyperthermic, encephalopathic, tachycardic, and hypotensive. On admission, she was found to have lactic acidosis, rhabdomyolysis, and acute kidney injury and was treated with cold normal saline and cooling blankets. She subsequently started having abdominal pain and bloody bowel movements. Computed tomography of the abdomen revealed ascending colon thickening. Furthermore, her lab findings showed transaminitis and elevated coagulation parameters. Due to the acute hypotensive state from the heat stroke, patient had developed bowel ischemia, ischemic hepatitis, and disseminated intravascular coagulation, all of which are uncommon complications of heat stroke. She was managed aggressively with intravenous fluid hydration with resolution of her symptoms over the course of 4 days. In addition to the uncommon complications, early presentation of this bowel ischemia despite adequate hydration in such a healthy individual is another unique aspect of the case. Umair Masood, Anuj Sharma, Wajihuddin Syed, and Divey Manocha Copyright © 2016 Umair Masood et al. All rights reserved. ILS Heuristics for the Single-Machine Scheduling Problem with Sequence-Dependent Family Setup Times to Minimize Total Tardiness Thu, 20 Oct 2016 07:34:39 +0000 http://www.hindawi.com/journals/jam/2016/9598041/ This paper addresses a single-machine scheduling problem with sequence-dependent family setup times. In this problem the jobs are classified into families according to their similarity characteristics. Setup times are required on each occasion when the machine switches from processing jobs in one family to jobs in another family. The performance measure to be minimized is the total tardiness with respect to the given due dates of the jobs. The problem is classified as -hard in the ordinary sense. Since the computational complexity associated with the mathematical formulation of the problem makes it difficult for optimization solvers to deal with large-sized instances in reasonable solution time, efficient heuristic algorithms are needed to obtain near-optimal solutions. In this work we propose three heuristics based on the Iterated Local Search (ILS) metaheuristic. The first heuristic is a basic ILS, the second uses a dynamic perturbation size, and the third uses a Path Relinking (PR) technique as an intensification strategy. We carry out comprehensive computational and statistical experiments in order to analyze the performance of the proposed heuristics. The computational experiments show that the ILS heuristics outperform a genetic algorithm proposed in the literature. The ILS heuristic with dynamic perturbation size and PR intensification has a superior performance compared to other heuristics. Vinícius Vilar Jacob and José Elias C. Arroyo Copyright © 2016 Vinícius Vilar Jacob and José Elias C. Arroyo. All rights reserved. The Extract of Chrysanthemum zawadskii var. latilobum Ameliorates Collagen-Induced Arthritis in Mice Thu, 20 Oct 2016 07:32:33 +0000 http://www.hindawi.com/journals/ecam/2016/3915013/ Chrysanthemum zawadskii var. latilobum (CZ) has been used for beverage or tea and also as folk medicine for the remedy of diverse inflammatory diseases. Nevertheless, the therapeutic effect of CZ on arthritis remains to be unknown. In this paper we aim to investigate the CZ’s antiarthritic effect and mechanism of action both in vitro and in vivo. To assess CZ’s antiarthritic effect, mouse models of type II collagen-induced arthritis (CIA) were used. Mice were used to gauge clinical arthritis index and histopathological changes. Reverse transcriptase-polymerase chain reaction (RT-PCR), western blotting, electrophoretic mobility shift assay (EMSA), and other biological methods were adopted to measure CZ’s effect on arthritis and to understand the veiled mechanism of action. CZ greatly suppressed CIA, histopathological score, bone erosion, and osteoclast differentiation. Mechanistically, CZ inhibited the production of various inflammatory and arthritic mediators like inflammatory cytokines, matrix metalloproteinases (MMPs), and chemokines. Of note, CZ significantly suppressed the activation of the NF-κB pathway in vivo. CZ exerted an antiarthritic effect in CIA mice by curbing the production of crucial inflammatory and arthritis mediators. This study warrants further investigation of CZ for the use in human rheumatoid arthritis (RA). A-Ram Kim, Hyuk Soon Kim, Do Kyun Kim, Jun Ho Lee, Young Hyo Yoo, Jeom Yong Kim, Sun Kyu Park, Seung Taek Nam, Hyun Woo Kim, Young Hwan Park, Dajeong Lee, Min Beom Lee, Young Mi Kim, and Wahn Soo Choi Copyright © 2016 A-Ram Kim et al. All rights reserved. Topic Modeling Based Image Clustering by Events in Social Media Thu, 20 Oct 2016 07:28:32 +0000 http://www.hindawi.com/journals/sp/2016/5283471/ Social event detection in large photo collections is very challenging and multimodal clustering is an effective methodology to deal with the problem. Geographic information is important in event detection. This paper proposed a topic model based approach to estimate the missing geographic information for photos. The approach utilizes a supervised multimodal topic model to estimate the joint distribution of time, geographic, content, and attached textual information. Then we annotate the missing geographic photos with a predicted geographic coordinate. Experimental results indicate that the clustering performance improved by annotated geographic information. Bin Xu, Guoliang Fan, and Dan Yang Copyright © 2016 Bin Xu et al. All rights reserved. Systemic Toxicity of Intraperitoneal Vancomycin Thu, 20 Oct 2016 06:51:54 +0000 http://www.hindawi.com/journals/crin/2016/3968690/ Intraperitoneal vancomycin is used for empiric treatment of peritoneal dialysis peritonitis. It is dosed intermittently and a high systemic concentration is often achieved. Despite this, there are very few reports of systemic toxicity from intraperitoneal vancomycin. We report the course of a patient who developed a drug reaction with eosinophilia and systemic symptoms (DRESS) syndrome after three weeks of intraperitoneal vancomycin. We review the literature and conclude that this is the first ever reported case of DRESS syndrome from intraperitoneal vancomycin. Teerath Kumar, Iris Teo, and Brendan B. McCormick Copyright © 2016 Teerath Kumar et al. All rights reserved. Hermite-Hadamard Type Inequalities Obtained via Fractional Integral for Differentiable -Convex and -Convex Functions Thu, 20 Oct 2016 06:51:24 +0000 http://www.hindawi.com/journals/ijanal/2016/4765691/ Some Hermite-Hadamard type inequalities involving fractional integrals for -convex and ()-convex functions are obtained. Erhan Set, Süleyman Sami Karataş, and Muhammad Adil Khan Copyright © 2016 Erhan Set et al. All rights reserved. Management of Psoriasis Herpeticum in Pregnancy: A Clinical Conundrum Thu, 20 Oct 2016 06:48:44 +0000 http://www.hindawi.com/journals/criog/2016/5319425/ Introduction. Kaposi varicelliform eruption (KVE) is a widespread cutaneous viral infection, most commonly herpes simplex virus, which affects patients with underlying dermatosis. When KVE occurs in a patient with a history of psoriasis, it is referred to as psoriasis herpeticum, a rare subtype of KVE with only a handful of cases reported in the literature. To the authors’ knowledge, we report for the first time a case of psoriasis herpeticum in pregnancy. Case Presentation. A 23-year-old woman in her third pregnancy presented at 26-week gestation with a 10-year history of psoriasis. Cutaneous examination revealed diffuse psoriatic plaques with scattered ~1 cm erosions. Punch biopsy of the skin revealed herpes simplex virus (HSV) infection within a psoriatic plaque, necessitating dermatological treatment. The patient experienced premature rupture of membranes at 37-week gestation. Pelvic exam showed no evidence of herpetic lesions. After labor augmentation, the patient delivered a healthy female infant with no evidence of HSV infection. Discussion. Psoriasis herpeticum is a rare and potentially devastating complication of an underlying dermatosis. With a paucity of data available to guide pregnancy-specific issues, the general management of this condition is controversial and requires a multidisciplinary care approach. Concerns for systemic infection in the mother and vertical transmission to the neonate are of critical importance. Leanne Almario, Albert S. Antonyan, Dennis A. Porto, Hunter Gomez-Roberts, Ali Alhousseini, and Bernard Gonik Copyright © 2016 Leanne Almario et al. All rights reserved. Prevalence and Prognosis of Anemia in Dogs with Degenerative Mitral Valve Disease Thu, 20 Oct 2016 06:37:23 +0000 http://www.hindawi.com/journals/bmri/2016/4727054/ In humans, heart failure (HF) and renal insufficiency (RI) have negative reciprocal effects, and anemia can exacerbate their progression. In this retrospective study, the prevalence and prognostic significance of anemia in 114 dogs with degenerative mitral valve disease (DMVD) was investigated. Pretreatment clinical parameters, prevalence of anemia and azotemia, and survival time were analyzed in relation to HF severity. The prevalence of anemia was highest in dogs with the modified New York Heart Association (NYHA) class IV HF (33.3%), followed by classes III (15.2%) and II (0%; p < 0.001). The presence of anemia was associated with HF severity and blood creatinine > 1.6 mg/dL (both p < 0.001). Anemic dogs had a shorter median survival [13 months; 95% confidence interval (CI): 0.7–19.1] than nonanemic dogs (28 months; 95% CI: 15.3–40.7; p < .001). NYHA class IV (hazard ratio (HR): 3.1, 95% CI: 2.2–4.3; p < 0.001), left atrium/aorta ratio > 1.7 (HR: 2.7, 95% CI: 1.7–4.2; p = 0.001), and presence of anemia (HR: 1.43, 95% CI: 1.1–1.9; p = 0.004) emerged as predictors of mortality. A cardiorenal-anemia syndrome-like triangle was observed and anemia was a prognostic factor for survival in dogs with DMVD. Ivarosa Bing-Ye Yu and Hui-Pi Huang Copyright © 2016 Ivarosa Bing-Ye Yu and Hui-Pi Huang. All rights reserved. The Effect of Mineral Trioxide Aggregate on the Periapical Tissues after Unintentional Extrusion beyond the Apical Foramen Thu, 20 Oct 2016 06:24:53 +0000 http://www.hindawi.com/journals/crid/2016/3590680/ Introduction. Single-step apexification procedures using mineral trioxide aggregate (MTA) have been reported as favorable treatment options for teeth with an open apex, posing greater benefits compared to the other available medicaments. However, controlled apical placement of MTA is a challenging procedure to perform using orthograde approach. This case series describes the outcome of the unintentional extrusion of MTA into periradicular tissues during apexification, in three separate cases. Methods. Three adult patients reported to the Department of Conservative Dentistry and Endodontics for the management of maxillary incisors with open apices. After isolation, conventional access, and cleaning and shaping procedures, one-step MTA apexification was performed. On subsequent radiographs, a considerable amount of MTA was seen to be extruded in all the three cases. Results. During follow-up examination the teeth were seen to be asymptomatic in all cases and radiographically demonstrated repair of the periapical lesion. Conclusion. The results of these cases suggest that extrusion of MTA into the periapical tissues does not cause any detrimental effect, which could be attributed to the biologic properties of MTA. Pradnya S. Nagmode, Archana B. Satpute, Ankit V. Patel, and Pushpak L. Ladhe Copyright © 2016 Pradnya S. Nagmode et al. All rights reserved. Atypical Diabetic Foot Ulcer Keratinocyte Protein Signaling Correlates with Impaired Wound Healing Thu, 20 Oct 2016 06:17:10 +0000 http://www.hindawi.com/journals/jdr/2016/1586927/ Diabetes mellitus is associated with chronic diabetic foot ulcers (DFUs) and wound infections often resulting in lower extremity amputations. The protein signaling architecture of the mechanisms responsible for impaired DFU healing has not been characterized. In this preliminary clinical study, the intracellular levels of proteins involved in signal transduction networks relevant to wound healing were non-biasedly measured using reverse-phase protein arrays (RPPA) in keratinocytes isolated from DFU wound biopsies. RPPA allows for the simultaneous documentation and assessment of the signaling pathways active in each DFU. Thus, RPPA provides for the accurate mapping of wound healing pathways associated with apoptosis, proliferation, senescence, survival, and angiogenesis. From the study data, we have identified potential diagnostic, or predictive, biomarkers for DFU wound healing derived from the ratios of quantified signaling protein expressions within interconnected pathways. These biomarkers may allow physicians to personalize therapeutic strategies for DFU management on an individual basis based upon the signaling architecture present in each wound. Additionally, we have identified altered, interconnected signaling pathways within DFU keratinocytes that may help guide the development of therapeutics to modulate these dysregulated pathways, many of which parallel the therapeutic targets which are the hallmarks of molecular therapies for treating cancer. Glenn D. Hoke, Corrine Ramos, Nicholas N. Hoke, Mary C. Crossland, Lisa G. Shawler, and Joseph V. Boykin Copyright © 2016 Glenn D. Hoke et al. All rights reserved. Influences by Air Voids on the Low-Temperature Cracking Property of Dense-Graded Asphalt Concrete Based on Micromechanical Modeling Thu, 20 Oct 2016 06:07:39 +0000 http://www.hindawi.com/journals/amse/2016/6942696/ This study characterized the impacts of air voids on the low-temperature cracking behavior of dense-graded asphalt concrete. Virtual low-temperature bending beam test for dense-graded asphalt concrete was built and executed by discrete element method and PFC3D (particle flow code in three dimensions). Virtual tests were applied to analyze the impacts by content, distribution, and size of air voids on the low-temperature properties of dense-graded asphalt concrete. The results revealed that higher air void content results in worse low-temperature property of dense-graded asphalt concrete, especially when the air void content exceeds the designed air content; even with the same designed air void content, different distributing condition of air voids within asphalt concrete leads to different low-temperature properties of asphalt concrete, especially when the air void content in the central-lower part of testing sample varies. Bigger size of single air void which tends to form interconnected air voids within asphalt concrete has more harmful impacts on the low-temperature properties of asphalt concrete. Thus, to achieve satisfied low-temperature properties of dense-graded asphalt concrete, it is critical to ensure the designed air void content, improve the distribution of air voids, and reduce the interconnected air voids for dense-graded asphalt concrete. Tao Ma, Yao Zhang, Hao Wang, Xiaoming Huang, and Yongli Zhao Copyright © 2016 Tao Ma et al. All rights reserved. Retroperitoneal Teratoma in an Adult: A Potential Pitfall in the Differential Diagnosis of Adrenal Myelolipoma Thu, 20 Oct 2016 06:03:50 +0000 http://www.hindawi.com/journals/criu/2016/5141769/ We report a 32-year-old female case of a right adrenal gland mass detected on CT scan at medical checkup. CT and MRI showed a mass of 5.1 cm made of fat and calcification in the right adrenal gland, leading to the clinical diagnosis of adrenal myelolipoma. Because of its relatively large size and the patient’s desire, the patient underwent gasless single-port retroperitoneoscopic adrenalectomy using the RoboSurgeon system. Histopathological examination revealed that the cystic tumor is composed of keratinized epidermis, mature fat, nerve, cartilage, bone, and sebaceous glands compressing the normal adrenal gland, leading to the diagnosis of retroperitoneal mature cystic teratoma. The patient remains free of recurrence 29 months after surgery. Retroperitoneal teratoma is relatively rare but clinically important because of high possibility of malignancy. In a case of an adrenal mass difficult to clinically distinguish retroperitoneal teratoma from adrenal myelolipoma, surgical resection via a minimally invasive approach would be the best therapeutic option. Madoka Kataoka, Hiroshi Fukushima, Yasukazu Nakanishi, Minato Yokoyama, Nobuaki Funata, Toru Motoi, Ken-ichi Tobisu, and Fumitaka Koga Copyright © 2016 Madoka Kataoka et al. All rights reserved. Gastrointestinal Disturbances Associated with the Consumption of Sugar Alcohols with Special Consideration of Xylitol: Scientific Review and Instructions for Dentists and Other Health-Care Professionals Thu, 20 Oct 2016 06:00:49 +0000 http://www.hindawi.com/journals/ijd/2016/5967907/ Sugar alcohols (polyols) are used in food manufacturing and in medical tests and examinations. d-Glucitol (sorbitol) and d-mannitol were previously the most common alditols used for these purposes. After the 1960s, xylitol became a common ingredient in noncariogenic confectioneries, oral hygiene products, and diabetic food. Erythritol, a polyol of the tetritol type, can be regarded as the sweetener of the “next generation.” The disaccharide polyols maltitol, lactitol, and isomalt have also been used in food manufacturing and in medical tests. Consumption of pentitol- and hexitol-type polyols and disaccharide polyols may cause gastrointestinal disturbances at least in unaccustomed subjects. The occurrence of disturbances depends on consumer properties and on the molecular size and configuration of the polyol molecule. Adaptation may take place as a result of enzyme induction in the intestinal flora. Some of the literature on xylitol has been difficult to access by health-care professionals and will be reviewed here. Research and clinical field experience have found no pathology in polyol-associated osmotic diarrhea—the intestinal mucosa having normal basic structure, except in extreme instances. Xylitol is better tolerated than hexitols or the disaccharide polyols. Erythritol, owing to its smaller molecular weight and configuration that differ from other alditols, normally avoids the gastrointestinal reactions encountered with other polyols. This review will also touch upon the FODMAPs diet concept. Kauko K. Mäkinen Copyright © 2016 Kauko K. Mäkinen. All rights reserved. Optimal Preview Control for a Class of Linear Continuous Stochastic Control Systems in the Infinite Horizon Thu, 20 Oct 2016 05:59:54 +0000 http://www.hindawi.com/journals/mpe/2016/7679165/ This paper discusses the optimal preview control problem for a class of linear continuous stochastic control systems in the infinite horizon, based on the augmented error system method. Firstly, an assistant system is designed and the state equation is translated to the assistant system. Then, an integrator is introduced to construct a stochastic augmented error system. As a result, the tracking problem is converted to a regulation problem. Secondly, the optimal regulator is solved based on dynamic programming principle for the stochastic system, and the optimal preview controller of the original system is obtained. Compared with the finite horizon, we simplify the performance index. We also study the stability of the stochastic augmented error system and design the observer for the original stochastic system. Finally, the simulation example shows the effectiveness of the conclusion in this paper. Jiang Wu, Fucheng Liao, and Jiamei Deng Copyright © 2016 Jiang Wu et al. All rights reserved. Comprehensive Comparison of the Performance of Autogenous Brachial-Basilic Transposition Arteriovenous Fistula and Prosthetic Forearm Loop Arteriovenous Graft in a Multiethnic Asian Hemodialysis Population Thu, 20 Oct 2016 05:58:46 +0000 http://www.hindawi.com/journals/bmri/2016/8693278/ Aim. For patients who have exhausted cephalic vein arteriovenous fistula (AVF) options, controversy exists on whether brachial-basilic AVF with transposition (BBTAVF) or a forearm arteriovenous graft (AVG) should be the next vascular access of choice. This study compared the outcomes of these two modalities. Methods. A retrospective study of 122 Asian multiethnic patients who underwent either a BBTAVF (81) or an AVG (41). Maturation time and intervention rates were analyzed. Functional primary, secondary, and overall patency rates were evaluated. Results. The maturation time for BBTAVFs was significantly longer than AVGs. There was also a longer deliberation time before surgeons abandon a failing BBTAVF compared to an AVG. Both functional primary and secondary patency rates were significantly higher in the BBTAVF group at 1-year follow-up: 73.2% versus 34.1% () and 71.8% versus 54.3% (), respectively. AVGs also required more interventions to maintain patency. When maturation rates were considered, the overall patency of AVGs was initially superior in the first 25 weeks after creation and then became inferior afterwards. Conclusion. BBTAVFs had superior primary and functional patency and required less salvage interventions. The forearm AVG might have a role in patients who require early vascular access due to complications from central venous catheters or with limited life expectancy. Koy Min Chue, Kyi Zin Thant, Hai Dong Luo, Yu Hang Rodney Soh, and Pei Ho Copyright © 2016 Koy Min Chue et al. All rights reserved. Spectral Gini Index for Quantifying the Depth of Consciousness Thu, 20 Oct 2016 05:58:07 +0000 http://www.hindawi.com/journals/cin/2016/2304356/ We propose indices that describe the depth of consciousness (DOC) based on electroencephalograms (EEGs) acquired during anesthesia. The spectral Gini index (SpG) is a novel index utilizing the inequality in the powers of the EEG spectral components; a similar index is the binarized spectral Gini index (BSpG), which has low computational complexity. A set of EEG data from 15 subjects was obtained during the induction and recovery periods of general anesthesia with propofol. The efficacy of the indices as indicators of the DOC was demonstrated by examining Spearman’s correlation coefficients between the indices and the effect-site concentration of propofol. A higher correlation was observed for SpG and BSpG (0.633 and 0.770, resp., ) compared to the conventional indices. These results show that the proposed indices can achieve a reliable quantification of the DOC with simplified calculations. Kyung-Jin You, Gyu-Jeong Noh, and Hyun-Chool Shin Copyright © 2016 Kyung-Jin You et al. All rights reserved. Bilateral Simultaneous Quadriceps Tendon Rupture in a 24-Year-Old Obese Patient: A Case Report and Review of the Literature Thu, 20 Oct 2016 05:57:34 +0000 http://www.hindawi.com/journals/crior/2016/4713137/ Introduction. Simultaneous bilateral quadriceps tendon ruptures (SBQTR) are uncommon knee injuries and most frequently occur in male patients, over 50 years of age. It can be associated with one or more predisposing risk factors like obesity, steroids use, and hyperparathyroidism. The main focus of this paper is to review SBQTR in obese patients. Case Report. We are reporting the youngest patient in the literature to date, a 24-year-old obese male patient, who presented to the emergency department complaining of bilateral knee pain and inability to walk after a fall during a basketball game. His clinical examination revealed the presence of a palpable suprapatellar gap and loss of knee extension bilaterally. Magnetic resonance imaging (MRI) confirmed that both of his quadriceps tendons were ruptured. A day after his diagnosis, the patient underwent successful operative repair followed by rehabilitation. At the two-year follow-up, the patient had full strength of both quadriceps muscles with no extension lag. Conclusion. The diagnosis of SBQTR can be challenging. Early diagnosis and treatment are associated with better functional outcome compared to delayed treatment. Physicians should have a high index of clinical suspicion in order not to miss such an injury and achieve favourable outcomes. Fahad H. Abduljabbar, Abdulaziz Aljurayyan, Bayan Ghalimah, and Lawrence Lincoln Copyright © 2016 Fahad H. Abduljabbar et al. All rights reserved. Steady-State Thermoelastic Analytical Solutions for Insulated Pipelines Wed, 19 Oct 2016 14:38:06 +0000 http://www.hindawi.com/journals/mpe/2016/6274509/ A steady-state thermoelastic analytical solution for a multilayer hollow cylinder, composed of an arbitrary number of phases and subject to both radial pressure and temperature gradient, is presented. By assuming each phase to be homogeneous and thermally isotropic and by varying the mechanical and thermal constitutive parameters, a sensitivity analysis has been performed with the aim of finally applying the study to the mechanical behaviour of an industrial pipeline composed of three phases (steel, insulating coating, and polyethylene) under the action of the above-mentioned load conditions. By making reference to a classical Hencky-von Mises criterion, the stress profiles along the thickness of the layers have been carried out, also localizing the onset of plasticity as a function of the temperature variations, material properties, and geometrical features characterizing the composite structure of interest. At the end, some numerical results of practical interest in the engineering applications have been specialized to three different insulated coating materials (expanded polyurethane, laminate glass, and syntactic foam), to highlight the cases in which thermal properties and loads can significantly interfere with the mechanical response in pipes, in terms of stresses, in this way suggesting possible strategies for avoiding unexpected failure and supporting the optimal structural design of these systems. M. Fraldi, L. Esposito, F. Carannante, A. Cutolo, and L. Nunziante Copyright © 2016 M. Fraldi et al. All rights reserved. Construction of the Global Solutions to the Perturbed Riemann Problem for the Leroux System Wed, 19 Oct 2016 14:30:52 +0000 http://www.hindawi.com/journals/amp/2016/4808610/ The global solutions of the perturbed Riemann problem for the Leroux system are constructed explicitly under the suitable assumptions when the initial data are taken to be three piecewise constant states. The wave interaction problems are widely investigated during the process of constructing global solutions with the help of the geometrical structures of the shock and rarefaction curves in the phase plane. In addition, it is shown that the Riemann solutions are stable with respect to the specific small perturbations of the Riemann initial data. Pengpeng Ji and Chun Shen Copyright © 2016 Pengpeng Ji and Chun Shen. All rights reserved. On the Impact of Tsallis Statistics on Cosmic Ray Showers Wed, 19 Oct 2016 14:13:58 +0000 http://www.hindawi.com/journals/ahep/2016/4676024/ We investigate the impact of the Tsallis nonextensive statistics introduced by intrinsic temperature fluctuations in -Air ultrahigh energy interactions on observables of cosmic ray showers, such as the slant depth of the maximum and the muon number on the ground . The results show that these observables are significantly affected by temperature fluctuations and agree qualitatively with the predictions of Heitler model. M. Abrahão, W. G. Dantas, R. M. de Almeida, D. R. Gratieri, and T. J. P. Penna Copyright © 2016 M. Abrahão et al. All rights reserved. Peri-Implant Bone Loss and Peri-Implantitis: A Report of Three Cases and Review of the Literature Wed, 19 Oct 2016 14:12:15 +0000 http://www.hindawi.com/journals/crid/2016/2491714/ Dental implant supported restorations have been added substantially to the clinical treatment options presented to patients. However, complications with these treatment options also arise due to improper patient selection and inadequate treatment planning combined with poor follow-up care. The complications related to the presence of inflammation include perimucositis, peri-implant bone loss, and peri-implantitis. Prevalence rates of these complications have been reported to be as high as 56%. Treatment options that have been reported include nonsurgical therapy, the use of locally delivered and systemically delivered antibiotics, and surgical protocols aimed at regenerating the lost bone and soft tissue around the implants. The aim of this article is to report on three cases and review some of the treatment options used in their management. Vanchit John, Daniel Shin, Allison Marlow, and Yusuke Hamada Copyright © 2016 Vanchit John et al. All rights reserved. The Influence of the Variety, Vineyard, and Vintage on the Romanian White Wines Quality Wed, 19 Oct 2016 14:09:02 +0000 http://www.hindawi.com/journals/jamc/2016/4172187/ The wine is one of the most consumed drinks over the world, being subjected to falsification or adulteration regarding the variety, vintage, and geographical region. In this study, the influence of different characteristics of wines (type, production year, and origin) on the total phenolic content, total flavonoids content, antioxidant activity, total sugars content, pH, and 18O/16O isotopic ratio was investigated. The differentiation of selected wines on the basis of tested parameters was investigated using chemometric techniques, such as analysis of variance, cluster analysis, and principal component analysis. The experimental results are in agreement with other outcomes and allow concluding that variety and vineyard have the major influence on the studied parameters, but, also, statistical interaction effect between year and vineyard and year and variety is observed in some cases. The obtained results have demonstrated that these parameters together with chemometric techniques show a significant potential to be used for discrimination of white wines. Anamaria Hosu, Veronica Floare-Avram, Dana Alina Magdas, Ioana Feher, Mihai Inceu, and Claudia Cimpoiu Copyright © 2016 Anamaria Hosu et al. All rights reserved. Investigation of the Unsteady Flow Behaviour on a Wind Turbine Using a BEM and a RANSE Method Wed, 19 Oct 2016 14:08:25 +0000 http://www.hindawi.com/journals/jre/2016/6059741/ Analyses of the unsteady flow behaviour of a 5 MW horizontal-axis wind turbine (HAWT) rotor (Case I) and a rotor with tower (Case II) are carried out using a panel method and a RANSE method. The panel method calculations are obtained by applying the in-house boundary element method (BEM) panMARE code, which is based on the potential flow theory. The BEM is a three-dimensional first-order panel method which can be used for investigating various steady and unsteady flow problems. Viscous flow simulations are carried out by using the RANSE solver ANSYS CFX 14.5. The results of Case I allow for the calculation of the global integral values of the torque and the thrust and include detailed information on the local flow field, such as the pressure distribution on the blade sections and the streamlines. The calculated pressure distribution by the BEM is compared with the corresponding values obtained by the RANSE solver. The tower geometry is considered in the simulation in Case II, so the unsteady forces due to the interaction between the tower and the rotor blades can be calculated. The application of viscous and inviscid flow methods to predict the forces on the HAWT allows for the evaluation of the viscous effects on the calculated HAWT flows. Israa Alesbe, Moustafa Abdel-Maksoud, and Sattar Aljabair Copyright © 2016 Israa Alesbe et al. All rights reserved. Impact of Anti-Inflammatory Drugs on Pyogenic Vertebral Osteomyelitis: A Prospective Cohort Study Wed, 19 Oct 2016 14:06:06 +0000 http://www.hindawi.com/journals/ijr/2016/9345467/ Objective. Pyogenic vertebral osteomyelitis (PVO) are frequently misdiagnosed and patients often receive anti-inflammatory drugs for their back pain. We studied the impact of these medications. Methods. We performed a prospective study enrolling patients with PVO and categorized them depending on their drugs intake. Then, we compared diagnosis delay, clinical presentation at hospitalization, incidence of complications, and cure rate. Results. In total, 79 patients were included. Multivariate analysis found no correlation between anti-inflammatory drug intake and diagnosis delay, clinical presentation, complications, or outcome. Conclusion. Anti-inflammatory drugs intake does not affect diagnostic delay, severity at diagnosis, or complications of PVO. Aurélien Dinh, Maxime Jean, Frédérique Bouchand, Benjamin Davido, Alexis Descatha, Clara Duran, Guillaume Gras, Christian Perronne, Denis Mulleman, Jérôme Salomon, and Louis Bernard Copyright © 2016 Aurélien Dinh et al. All rights reserved. Double-Layered Lateral Meniscus in an 8-Year-Old Child: Report of a Rare Case Wed, 19 Oct 2016 13:57:29 +0000 http://www.hindawi.com/journals/crior/2016/5263248/ Reports of congenital abnormalities of the lateral meniscus include discoid meniscus, accessory meniscus, double-layered meniscus, and ring-shaped meniscus. Particularly, only a few cases of double-layered meniscus have been reported. We report a case of double-layered lateral meniscus, in which an additional semicircular meniscus was observed under the normal lateral meniscus. The accessory hemimeniscus was resected by means of arthroscopic surgery. This case demonstrates an interesting and extremely rare anatomical abnormality of the lateral meniscus. Susumu Araki, Mitsuhiko Kubo, Kosuke Kumagai, and Shinji Imai Copyright © 2016 Susumu Araki et al. All rights reserved. Lay Health Trainers Supporting Self-Management amongst Those with Low Heath Literacy and Diabetes: Lessons from a Mixed Methods Pilot, Feasibility Study Wed, 19 Oct 2016 13:42:01 +0000 http://www.hindawi.com/journals/jdr/2016/4723636/ This article reports a mixed methods process evaluation of a pilot feasibility randomised controlled trial comparing a Lay Health Trainer (LHT) intervention and usual care for those with poorly controlled Type 2 Diabetes Melitus (T2DM). Set in a deprived area in the UK, this research explores patient and health care practitioner (HCP) views on whether a structured interview between a patient and a Lay Health Trainer (LHT), for the purpose of developing a tailored self-management plan for patients, is acceptable and likely to change health behaviours. In doing so, it considers the implications for a future, randomised controlled trial (RCT). Participants were patients, LHTs delivering the intervention, service managers, and practice nurses recruiting patients to the study. Patients were purposively sampled on their responses to a baseline survey, and semistructured interviews were conducted within an exploratory thematic analysis framework. Findings indicate that the intervention is acceptable to patients and HCPs. However, LHTs found it challenging to work with older patients with long-term and/or complex conditions. In order to address this, given an ageing population and concomitant increases in those with such health needs, LHT training should develop skills working with these populations. The design of any future RCT intervention should take account of this. Bernadette Bartlam, Trishna Rathod, Gillian Rowlands, and Joanne Protheroe Copyright © 2016 Bernadette Bartlam et al. All rights reserved. Serum Lipoprotein (a) Levels in Black South African Type 2 Diabetes Mellitus Patients Wed, 19 Oct 2016 13:41:39 +0000 http://www.hindawi.com/journals/omcl/2016/5743838/ Lipoprotein (a) (Lp(a)) which is a low-density lipoprotein-like particle containing apo(a) is considered as an emergent cardiovascular risk factor. Type 2 diabetes mellitus (T2DM) is associated with a two- to threefold increase in the risk of cardiovascular disease (CVD). The aim of this study was to investigate the levels of Lp(a) in Black South African T2DM patients and its association with other metabolic factors. 67 T2DM patients and 48 healthy control participants were recruited for the cross-sectional study. The Lp(a) level was determined by ELISA and the result was analyzed using SPSS. The Lp(a) level in diabetics was found to be significantly increased () when compared to the normal healthy group. In the diabetic group, the Lp(a) levels correlated significantly with the duration of diabetes () and oxidized LDL (ox-LDL) levels () and decreased total antioxidant capacity (). The third tertile of Lp(a) was significantly correlated with increased ox-LDL, C-reactive protein, and triglycerides and decreased total antioxidant capacity. Jim Joseph, Farzana Ganjifrockwala, and Grace George Copyright © 2016 Jim Joseph et al. All rights reserved. Instrumental and Sensory Analysis of the Properties of Traditional Chinese Fried Fritters Wed, 19 Oct 2016 13:41:04 +0000 http://www.hindawi.com/journals/jchem/2016/3626581/ The quality of traditional Chinese fried fritters is typically measured using human sensory evaluation techniques and physicochemical indices, the process of which is laborious and time-consuming. This study aimed to investigate the relationship between instrumental parameters, sensory criteria, and physicochemical indices. Significant correlations were found using principle component analysis. Volume, fat, texture, palatability, and instrumental parameters (hardness, fracturability, springiness, and gumminess) were found to be the main factors influencing the quality of Chinese fried fritters by principal component analysis (PCA) and instrumental methods, which were satisfactory replacement for human evaluation in correlation testing. Daming Fan, Bowen Yan, Huizhang Lian, Jianxin Zhao, and Hao Zhang Copyright © 2016 Daming Fan et al. All rights reserved.