Aquaporin 5 Expression in Mouse Mammary Gland Cells Is Not Driven by Promoter Methylation

Figure 2

Cell growth and AQP5 expression at different cell culturing stages. (a) Cell culturing and treatment times at which cells were harvested (indicated with arrows). (b) Numbers of cultured cells were counted with a Coulter Counter. Shown are averages (dots) and confidence intervals; ; alpha = 0.05. (c) Total mRNA was extracted from cells used for seeding, 12 h after seeding, 26 h after seeding (with or without 2 h Dex treatment), 36 h after seeding with 12 h Matrigel treatment, and 50 h after seeding with 26 h Matrigel treatment (with or without 2 h Dex treatment). AQP5 expression is normalized to GAPDH and shown relative to the 0 time point treatment. Bars represent averages and confidence intervals; ; alpha = 0.05. Significance was tested between the 26 h treatments (−Dex, −Matrigel, 26 h and 2 h Dex, −Matrigel, 26 h) and between the 50 h treatments (−Dex, 26 h Matrigel, 50 h and 2 h Dex, 26 h Matrigel, 50 h).

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