Aquaporin 5 Expression in Mouse Mammary Gland Cells Is Not Driven by Promoter Methylation

Figure 4

Changes in DNA methylation with the demethylating agent 5-Aza in actively dividing cells. (a) Changes in DNA methylation monitored by bacterial bisulfite sequencing of individual clones. Cells were treated with 5-Aza for 48 h while still dividing. Data for the control (no Aza) are combined from two experiments, one treated with Matrigel (also shown in Supplementary Figure 9, treatment 1) and one without Matrigel. (b) Expression of AQP5 mRNA analyzed with qPCR. AQP5 expression is normalized to GAPDH and shown relative to the no-5-Aza treatment. Bars represent averages and confidence intervals; ; alpha = 0.05. Significance was tested between control (5-Aza) and the 5-Aza treated sample.

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