Aquaporin 5 Expression in Mouse Mammary Gland Cells Is Not Driven by Promoter Methylation

Figure 6

Effect of Dex on the AQP5 promoter. EpH4 cells were treated with Dex, 4 h prior () or during transfection () of the luciferase expression pGL3-basic vector including the AQP5 promoter (FF) and the pRL-TK transfection control plasmid (R). Negative controls included the pGL3-basic vector without promoter (cFF) () and the peGFP-N1 vector (GFP), (). (a) Levels of AQP5 mRNA normalized to GAPDH are shown for the different transfected EpH4 cells with or without Dex treatment. Bars represent averages and confidence intervals; alpha = 0.05. Significance was tested between samples without Dex and with Dex treatment of the same transfection type. (b) Levels of Firefly enzyme activity normalized to Renilla enzyme activity measured with the luciferase reporter assay for the same transfected cells as in panel (a). Bars represent averages and confidence intervals; alpha = 0.05. Significance was tested between controls (−Dex) and treatments (+Dex), with one or two asterisks denoting or , respectively.

(a)

(b)