A Drosophila Model for Screening Antiobesity Agents

Figure 6

Effects of oral administration of histone deacetylase (HDAC) inhibitors on bmm expression in Drosophila. The transgenic flies were fed a diet containing HDAC inhibitors, and the salivary glands of the third-instar larvae were observed by fluorescence microscopy to evaluate bmm promoter-driven GFP signaling as a marker. GFP signals of Drosophila fed with instant food alone (a), instant food with NCC-149 (HDAC8 inhibitor) (b), or instant food with T302 (an HDAC9 inhibitor) (c). The GFP intensity measured by the MetaMorph software was enhanced in flies fed the HDAC inhibitors, and significant differences were observed relative to the control (d). for each treatment. The error bars represent the standard deviation. ; .

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(b)

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(d)