Anti-Biofilm Effect of Ampicillin-Loaded Poly (Lactic-co-glycolic Acid) Nanoparticles Conjugated with Lysostaphin on Methicillin-Resistant Staphylococcus aureus
Table 1
Components and technological parameters of various formulations.
Formulation
AMP (mg)
LYS (mg)
PLGA (mg)
DCM1 (ml)
Chl2 (ml)
PVA (ml)
Size (nm)
PDI
ZP (mV)
EE (%)
DL (%)
LCE3 (%)
F1
6
—
30
20
—
20 (1%)
724.4 ± 45
0.372 ± 0.021
−18.5 ± 1.6
92.24 ± 7.3
13.91 ± 1.2
—
F2
6
—
30
—
15
20 (1%)
435.4 ± 23
0.280 ± 0.014
−17.3 ± 1.5
93.43 ± 5.2
15.53 ± 1.4
—
F3
12
—
60
—
15
20 (2%)
321.2 ± 36
0.274 ± 0.012
−20.5 ± 2.1
93.50 ± 4.5
16.42 ± 2.3
—
F4
24
3
120
—
15
20 (2%)
301.9±32
0.261±0.010
−19.2±3.4
94.53±3.8
18.91±1.6
40
1Dichloromethane. 2Chloroform. 3Lysostaphin conjugation efficiency. Bold values represent the best formulation used in the study.
