Decreased S100A9 Expression Promoted Rat Airway Smooth Muscle Cell Proliferation by Stimulating ROS Generation and Inhibiting p38 MAPK

Figure 3

Quantification of oxidative stress in airway smooth muscle cells (ASMCs) with stable knockdown of S100A9 expression (ASMC-shRNA-S100A9). (a) Effects of antioxidative agent N-acetylcysteine (NAC) on ASMC-shRNA-S100A9 proliferation at 24 h, 48 h, and 72 h. indicates a significant difference as compared to control (). (b) Comparison of the ratio of DCFH-DA fluorescence to cell area in ASMCs and ASMC-shRNA-S100A9 cells. The fluorescence of forty cells from three microscope fields was measured and the assay was repeated in triplicate. indicates a significant difference as compared to ASMCs (). All data are presented as the means ± SD.

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