Research Article
Cytotoxic Effects of Biosynthesized Zinc Oxide Nanoparticles on Murine Cell Lines
Figure 4
Fluorescent micrograph of AO/PI double stained WEHI-3B cells that was treated with ZnO-NPs. (a) Untreated cells showing normal cell structure. (b) Early apoptotic cells after 24 h treatment showing membrane blebbing and chromatin condensation. (c) Blebbing and nuclear margination after 48 h treatment. (d) DNA fragmentation and apoptotic body formation after 72 h treatment. VC: viable cells; EA: early apoptotic cells; CC: chromatin condensation; BL: blebbing; MN: marginated nucleus; FN: fragmented nucleus; LA: late apoptotic cells; and AB: apoptotic body.
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