Cytotoxic Effects of Biosynthesized Zinc Oxide Nanoparticles on Murine Cell Lines

Table 1

Flow cytometric analysis of WEHI-3B cells after treated with ZnO-NPs. The cells were stained with FITC-conjugated annexin V and PI and incubated at 37°C for 12, 24, and 48 h, respectively.

Cells (%)
Cell condition	Control; 12 h	Treated; 12 h	Control; 24 h	Treated; 24 h	Control; 48 h	Treated; 48 h
Viable cells	96.4 ± 0.75	80.59 ± 0.65	92.33 ± 0.55	78.29 ± 0.15	90.78 ± 0.25	73.5 ± 0.13
Early apoptosis	1.87 ± 0.15	8.75 ± 0.99^*	2.98 ± 0.70	10.00 ± 0.30^*	6.15 ± 0.45	15.5 ± 0.22^*
Late apoptosis necrosis	1.69 ± 0.35	10.66 ± 0.95^**	4.69 ± 0.50	11.7 ± 0.80^**	3.07 ± 0.25	10.9 ± 0.10^**

Values are expressed as mean ± SD of three different experiments. The data has been analyzed using post hoc comparison test one-way ANOVA; means were compared by Tukey’s test. ^*Significant ( < 0.05) increased early apoptotic cells in ZnO-NPs treated groups compared to untreated controls. ^**Significant ( < 0.05) increased late apoptotic/necrotic cells in ZnO-NPs-treated groups compared to untreated controls.