Cytotoxic Effects of Biosynthesized Zinc Oxide Nanoparticles on Murine Cell Lines

Table 2

Flow cytometric analysis of WEHI-3B cells after treated with ZnO-NPs. The cells were stained with PI and incubated at 37°C for 24, 48, and 72 h.

Cells (%)
Cell cycle phases	Control; 24 h	Treated; 24 h	Control; 48 h	Treated; 48 h	Control; 72 h	Treated; 72 h
G0/G1	55.25 ± 0.06	33.40 ± 0.45	51.10 ± 0.29	42.61 ± 0.52	50.64 ± 0.32	37.68 ± 0.68
G2/M	18.85 ± 0.76	18.00 ± 0.41	19.16 ± 0.26	12.29 ± 0.35	9.30 ± 0.22	6.06 ± 0.93
Synthesis	24.91 ± 0.06	38.30 ± 0.33	29.24 ± 0.06	22.93 ± 0.12	37.50 ± 0.61	30.35 ± 0.18
Sub-G0/G1	0.9 ± 0.23	10.50 ± 0.28^*	0.50 ± 0.34	22.00 ± 0.20^*	2.20 ± 0.46	25.85 ± 0.56^*

Values are expressed as mean ± SD of three different experiments. The data has been analyzed using post hoc comparison test one-way ANOVA; means compared with Tukey’s test. ^*Significant ( < 0.05) increased cells in sub-G0/G1 phase in ZnO-NPs treated groups compared to untreated controls.