Cryo-Imaging and Software Platform for Analysis of Molecular MR Imaging of Micrometastases

Figure 1

Experimental design for evaluation of CREKA imaging agents for a metastatic breast cancer model. GFP-labeled, 4T1 cells were injected in the fat pad. CREKA-Cy5 was injected in the tail vein for fluorescence evaluation. After 3 hours to allow clearance of nonbound agent, CREKA-Gd was injected. With MRI, we captured in vivo and high resolution ex vivo image volumes. After embedding in the freezing medium and snap freezing, we acquired microscopic, anatomical, colored episcopic and molecular, multispectral fluorescence cryo-image volumes. Optionally, selected histological sections were manually acquired using a tape system.