Research Article

A Chromosomal-Level Genome of Dermatophagoides farinae, a Common Allergenic Mite Species

Table 1

Assembly statistics of the Dermatophagoides farinae genome.

D. farinae 2.0 (GenBank no. GCA_000767015.2)UMICH_USM_1.1 (GenBank no. GCA_002085665.2)ASM2080927v1 (GenBank no. GCA_020809275.1)Our work (GenBank no. GCA_024713945.1)
ScaffoldContigScaffoldContigScaffoldContigScaffoldContig

Total number101081704172010102122
Total length (bp)6039494560120219918917129189121458776842587768426242831462428214
Gap number (bp)27478105890001000
N50 length (bp)898149022938411925011888699265486926548671179905241854
N90 length (bp)436661760675920380193373602211360221133489463348946
Maximum length (bp)16911843399594617407221740722137989241379892491729539172953
Minimum length (bp)3315592027067066138826138828972789727
GC content (%)30.5830.5830.4930.4930.3830.3830.5730.57
BUSCO (v5.3.2, arachnida_odb10)C: 91.4% (S: 89.4%, D: 2.0%), F: 4.0%, M: 4.6%, : 2934C: 92.4% (S: 72.7%, D: 19.7%), F: 3.5%, M: 4.1%, : 2934C: 91.7% (S: 90.4%, D: 1.3%), F: 3.7%, M: 4.6%, : 2934C: 92.1% (S: 90.9%, D: 1.2%), F: 3.6%, M: 4.3%, : 2934
Sequencing technologyOxford Nanopore GridION; PacBio SequelPacBio; Illumina HiSeqNanoporePacBio; Illumina HiSeq; Hi-C
Assembly methodFlye v. 2.6HGAP3 v. 2013WTDBG v. 1.2.8FALCON v. 0.3.0; WTDBG2 v. 2.5; Flye v. 2.0
Genome coverage431.0x100.0x396.0x265.0x
Assembly levelScaffoldScaffoldContigChromosome
Assembly release dateFeb 2022Apr 2017Nov 2021Aug 2022

C: complete BUSCOs; S: complete and single-copy; D: complete and duplicated BUSCOs; F: fragmented BUSCOs; M: missing BUSCOs.