Hyperexcitability in Spinal WDR Neurons following Experimental Disc Herniation Is Associated with Upregulation of Fractalkine and Its Receptor in Nucleus Pulposus and the Dorsal Root Ganglion

Table 1

Primer sequences.

Primer	Sequence 5′  3′	Bp	% GC	Tm °C
IL1β forward	CGT GGA GCT TCC AGG ATG AG	20	60.0	59.4
IL1β reverse	CGT CAT CAT CCC ACG AGT CA	20	50.0	59.1
TNF forward	GCC ACC ACG CTC TTC TGT CTA	21	57.1	59.1
TNF reverse	TGA GAG GGA GCC CAT TTG G	19	57.9	59.6
Csf1 forward	GGG AAT GGA CAC CTA CAG ATT TTG	24	45.8	59.6
Csf1 reverse	AAA TTT ATA TTC GAT CAG GCA TGCA	25	32.0	59.7
CX3CL1 forward	TTG CAC AGC CCA GAT CAT TC	21	47.6	54.3
CX3CL1 reverse	CTG CGC TCT CAG ATG TAG GAA A	22	50.0	55.7
CX3CR1 forward	GTG GCC TTT GGG ACC ATC T	19	57.9	56.7
CX3CR1 reverse	CCA CCA GAC CGA ACG TGA A	19	57.9	56.6
β-actin forward	CTA AGG CCA ACC GTG AAA AGA	21	47.6	58.0
β-actin reverse	ACA ACA CAG CCT GGA TGG CTA	21	52.4	59.2