N-Acetylcysteine Decreases Myocardial Content of Inflammatory Mediators Preventing the Development of Inflammation State and Oxidative Stress in Rats Subjected to a High-Fat Diet

Figure 3

The expression of proteins involved in the eicosanoid synthesis pathway, i.e., (a) cyclooxygenase-1 (COX-1), (b) cyclooxygenase-2 (COX-2), (c) 5-lipoxygenase (5-LOX), and (d) 12/15-lipoxygenase (12/15-LOX) after eight-week N-acetylcysteine (NAC) treatment in the left ventricle tissue of rats fed a standard diet (control) or a high-fat diet (HFD). The expression of selected proteins was examined by the Western blot method. The data are expressed as mean ± standard deviation (SD) and based on six independent determinations (n = 6). The significant differences were assessed by two-way ANOVA followed by a respective post hoc test (Tukey’s test and t-test). , significant difference compared to the control group; ^#, significant difference compared to the HFD group.

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