Characterization of a New Immunosuppressive and Antimicrobial Peptide, DRS-DA2, Isolated from the Mexican Frog, Pachymedusa dacnicolor

Figure 10

Bacterial membrane permeabilization. (a) E. coli ML35p inner membrane permeabilization by DRS-DA2 peptides at a 2 × MIC concentration in comparison with melittin or omiganan revealed by hydrolysis of ONPG. (b) Depolarization effects of DRS-DA2N, DRS-DA2NEQ, and melittin on the E. coli cytoplasmic membrane. (c) ANTS/DPX assay with DRS-DA2 peptides in comparison with melittin and omiganan. (d) Tryptophan blue shift (Δλmax) in the presence of liposomes produced from E. coli ML35p and DRS-DA2N or DRS-DA2NEQ peptides.

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