Characterization of a New Immunosuppressive and Antimicrobial Peptide, DRS-DA2, Isolated from the Mexican Frog, Pachymedusa dacnicolor

Figure 3

DRS-DA2N and DRS-DA2NEQ induce the death of human leukocytes. (a) Different populations of human leukocytes (PMN, Mo, NK, and T cells) were incubated for two hours at 37°C, 5% CO₂ in the presence of various concentrations of DRS-DA2N, DRS-DA2NEQ, or Scr. The percentage of live cells was determined by flow cytometry. (b) Total human leukocytes were incubated in the presence of DRS-DA2N, DRS-DA2NEQ, and Scr at a concentration of 10 µM for different time periods until 2 hours, and the percentage of live cells was determined by flow cytometry. Data represent three independent experiments run in triplicate.

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