Characterization of a New Immunosuppressive and Antimicrobial Peptide, DRS-DA2, Isolated from the Mexican Frog, Pachymedusa dacnicolor

Figure 4

DRS-DA2N and DRS-DA2NEQ are not toxic for erythrocytes or epithelial cells. (a) Human erythrocytes in PBS were incubated with various concentrations of DRS-DA2N and DRS-DA2NEQ for one hour. Triton 1% was used as a positive control. Hemoglobin release was monitored by measuring the absorbance at 550 nm (mean of 3 independent experiments run in triplicate). (b) Human primary epithelial cells were incubated with DRS-DA2N and DRS-DA2NEQ at the indicated concentrations for 2 hours at 37°C, 5% CO₂. The percentage of live cells was determined by flow cytometry. (c) A549 cells were incubated for 1 hour at 37°C, 5% CO₂ in the presence of increasing concentrations of the peptides. The percentage of live cells was determined by the CCK8 assay. Experiments were run in duplicate.

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