Characterization of a New Immunosuppressive and Antimicrobial Peptide, DRS-DA2, Isolated from the Mexican Frog, Pachymedusa dacnicolor

Figure 9

DRS-DA2N induces the death of immune cells by membrane disruption. Jurkat E6-1 cells were incubated for two hours with DRS-DA2N, Scr, or PBS or exposed to UV for 10 min and put to rest for 4 hours, 5% CO₂ at 37°C, before being stained with annexin V and the viability dye eFluor 780. (a) Representative flow cytometry analysis of Jurkat E6-1 cells double stained with annexin V (A) and the viability dye eFluor 780 (V). (b) Percentage of cells A⁻V⁻, A⁺V⁻, A⁻ V⁺, and A⁺V⁺ after the different treatments. Two experiments were run in triplicate.

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(b)