Characterization of a New Immunosuppressive and Antimicrobial Peptide, DRS-DA2, Isolated from the Mexican Frog, Pachymedusa dacnicolor

Table 2

Antimicrobial activities of DRS-DA2N and DRS-DA2NEQ on Gram-negative and Gram-positive strains.

	Antimicrobial activity: MIC/MBC (pM)
	DRS-DA2N		DRS-DA2NEQ		SAT		Melittin
	MIC	MBC	MIC	MBC	MIC	MBC	MIC	MBC
Gram-negative strains
E. coli ATCC 8739	0.8	0.8	1.6	1.6	>50	>50	3.2	3.2
E. coli K12	3.2	6.3	12.5	12.5	n.d	n.d	12.5	12.5
E. coli ML35p	0.8	0.8	1.6	1.6	n.d	n.d	12.5	12.5
E. coli P7 (BLSE)	1.6	12.5	12.5	50	n.d	n.d	6.3	6.3
P. aeruginosa ATCC 9027	3.2	3.2	6.3	6.3	>50	>50	6.3	6.3
P. aeruginosa ATCC 27853	12.5	50	50	>100	n.d	n.d	50	>50
K. pneumoniae CIP 52.211	0.8	0.8	0.8	0.8	n.d	n d	12.5	12.5
K. oxytoca CIP 7932	3.2	6.3	12.5	>100	n.d	n.d	zs	No
S. enterica CIP 8297	12.5	25	50	100	n.d	n.d	50	>50
Y. ruckeri ATGG 29473	3.2	12.5	25	>100	n.d	n.d	6.3	12.5
Gram-positive strains
S. aureus ATCC 6538	1.6	3.2	6.3	12.5	>50	>50	6.3	6.3
S. aureus MRSA	25	>50	100	>100	n.d	n.d	6.3	12.5
S. aureus ST 1065	6.3	6.3	12.5	25	n.d	n.d	10	10
S. epidermidis BM 3302	6.3	25	12.5	>50	n.d	n.d	10	10
L. monocytogenes SOR 100	25	25	100	>100	n.d	n.d	1.5	3.2
E. faecalis CIP A186	50	>50	100	>100	n.d	n.d	12.5	12.5
L. garvieae ATCC 43921	25	50	100	>100	n.d	n.d	6.3	25
E. faecalis CIP 103015	6.3	6.3	12.5	12.5	>50	>50	1.6	1.6
K. rhizophila ATCC 9341	0.8	1.6	1.6	1.s	n.d	n.d	0.8	0.8

Multiresistant strains.