Research Article

Effects of Long-Term Cultivation on Medium with Alpha-Ketoglutarate Supplementation on Metabolic Processes of Saccharomyces cerevisiae

Table 1

Wet biomass and levels of α-dicarbonyl compounds (DC) and triacylglycerides (TAG) in S. cerevisiae cells during prolonged cultivation in the control or 10 mM AKG-supplemented medium.

ParameterGrowth conditions
Control10 mM AKG
18 h42 h7 d18 h42 h7 d

Wet biomass (mg/108 cells)6.90 ± 0.81NSNS7.90 ± 0.27NSNS
TAG, μg/108 cells16.5 ± 1.115.2 ± 2.315.2 ± 1.615.6 ± 1.718.5 ± 3.116.7 ± 2.5
DC, nmol glyoxal equivalents/mg proteinNSNS7.01 ± 0.33NSNS5.53 ± 0.38

Results are shown as means ± SEM ( = 6–8). different from respective control values with using Student’s -test. NS: not studied.