Research Article
Insulin-Like Growth Factor I Prevents Cellular Aging via Activation of Mitophagy
Figure 7
PINK1 silencing blocked IGF-1-induced effect on cellular aging. P20 SMC was transfected with 5 nM PINK1 siRNA (black bars) or scrambled siRNA (control, grey bars), and after 48 hours, cells were exposed to IGF-1. Activity of senescence-associated β-galactosidase (SA-β-gal) (a), cytochrome C oxidase (b), mitochondrial membrane potential (MMP) (c), mitochondrial DNA damage (d), and DNA telomere length (e) was quantified using commercial assays. for SMC + IGF-1 vs. no IGF-1.
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