DGCR8 Localizes to the Nucleus as well as Cytoplasmic Structures in Mammalian Spermatogenic Cells and Epididymal Sperm

Figure 8

Localization of DGCR8 in the head and neck regions of rat late spermatids by IEM and the corresponding area stained by IF (inset). All data obtained with antibody from ProteinTech. (a) Apical area of a step 17 spermatid head. Gold particles are present on dense material in the cytoplasm of a Sertoli cell at the ectoplasmic specialization (bracket). The corresponding areas stained by IF are shown in inset (arrows). (b) A neck granule in a step 15 spermatid, which corresponds to granules stained by IF (Inset) (long arrows). Gold labeling is seen in the granule (short arrow) but not on cross-sectioned annulus (arrowheads). (c) Two neck granules are shown. They are positive for DGCR8 (arrows). (d) IEM control. No gold labeling is observed in a neck granule (arrow). Arrowheads indicate a cross-sectioned annulus. (e) Routine transmission EM image of neck region. A neck granule attaching to neck piece of flagellum is observed (arrow). A cross-sectioned annulus is pointed by arrowheads. N: nucleus. Bar = 0.5 μm.