Circulating miR-320b Contributes to CD4+ T-Cell Proliferation in Systemic Lupus Erythematosus via MAP3K1

Figure 5

MSCs regulate miR-320b/MAP3K1 expression both in vitro and in vivo. (a) PBMCs levels of MAP3K1 in SLE patients receiving MSCT. n = 8. (b) The changes in MAP3K1 expression in responders (n = 5) and nonresponders (n = 3) during MSCT. (c–f) PBMCs from HC were isolated, stimulated, and cocultured with or without MSCs for 4 days. The expression levels of miR-320b in PBMCs (c) and coculture medium (d) and MAP3K1 in PBMCs (e) were compared between two groups. The proliferation rate of CD4+ T cells was measured by flow cytometry (f). (c) n = 9; (d) n = 7; (e) n = 10. (g) PBMCs from HC were isolated, stimulated, and cultured in the presence of MSCs or RNase for 4 days. The proliferation rate of CD4+ T cells was measured by flow cytometry. n = 3. MSCT, mesenchymal stem cell transplantation; PBMC, peripheral blood mononuclear cell. , , .

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