CD4⁺T and CD8⁺T Cells in Uterus Exhibit Both Selective Dysfunction and Residency Signatures

Figure 7

The immunological characteristics of CD4⁺T and CD8⁺T cells from human endometrium and blood. Isolated cells were incubated with respective mAbs and the percentages of T cell subsets were detected by flow cytometry. The representative dot plots (a) and pie chart (n = 6) (b) for the frequencies of CD4⁺T, CD8⁺T, and other T cells in human endometrium and PBMC were shown. The representative dot plots showed the frequencies of CD45RO, CD69, and CD103-expressing CD4⁺T cells (c) and CD8⁺T cells (d) from human endometrium and blood. Data were representative of three independent experiments. Isolated cells from human endometrium were stimulated with or without PMA and Ionomycin in the existence of BFA for 6 hr and then assessed the expression of IFN-γ and IL-17. The frequencies of IFN-γ and IL-17-expressing CD4⁺T cells (e) and CD8⁺T cells (f) from human endometrium and blood were shown. Data were presented as mean ± SEM and compared with the Student’s t-test. NS, no significance; .

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