Affect cleavage efficiency, toxicity, and immunologic responsiveness and produce sequence-independent effects
Substitution of oxygen atoms with sulfur atoms affects the DNAzyme structure in a molecularity-dependent manner Counteract the degradation by exonucleases
Increase affinity for complementary sequence, increase stability, solubility, easily automated synthesis, and straightforward cellular delivery
Influence catalytic activity and biological potency
Increase in stability due to efficient base stacking by adopting A-form geometry and oxymethylene bridge link between 2 and 4 carbon atoms of a furanose ring Charged backbone facilitating lucid cellular transfection A change in the charge distribution of the minor groove wall furnish solvation properties