Transcriptomic Modification in the Cerebral Cortex following Noninvasive Brain Stimulation: RNA-Sequencing Approach

Figure 6

Weighted Gene Coexpression Network Analysis (WGCNA). (a) Network heatmap plot. Each branch in the hierarchical clustering dendrograms corresponds to each of 15 modules determined by the WGCNA. The color bars between the dendrograms and the heatmap plot represent the color-coded eigengene-based connectivity. Progressively more red color indicates highly interconnected genes in each module. (b) Heatmap plot of the adjacencies in the eigengene network including the trait tDCS current intensities. Each row and column in the heatmap indicate one module eigengene and the last row and column represent the trait tDCS current intensities. Negative and positive correlation are represented by green and red colors, respectively, as shown on the indicator (range: 0.0–1.0). (c) Module-trait (tDCS current intensity) relationship. Each row corresponds to a module eigengene, and each cell contains the corresponding correlation (top) and value (bottom in parentheses) calculated by the WGCNA package. (d) A scatterplot of gene significance (GS) for tDCS intensity versus module membership (MM) in the brown module. A highly significant correlation between GS and MM in the brown module (correlation = 0.74; value = ) was detected. (e) The network connections among the most connected genes in the brown module, generated by the VisANT software (version 5.5). The plot shows network connections whose topological overlap is above the threshold of 0.35, and the red-colored genes are the top 3 genes that have the greater number of connections.

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