Role of Reactive Oxygen Species in the Neural and Hormonal Regulation of the PNMT Gene in PC12 Cells

Figure 2

Effect of NAC pretreatment on levels of PNMT mRNA, intracellular ROS, and extracellular NO in PC12 cells treated with 200 μM CoCl₂ for 24 h at 37°C. (a) Representative image (𝑛=3) of PNMT (intron-retaining and intronless splice variants) mRNA levels from semiquantitative RT-PCR. GAPDH mRNA was used as a housekeeping control. (b) PNMT intron-retaining mRNA and (c) intronless mRNA. Data is normalized to GAPDH control. (d) Intracellular ROS levels and (e) NO concentration after 24 h. Data presented as relative fold change or NO concentration ± SEM from a minimum of three independent experiments. Significance defined as ^*/+𝑃<0.05; ^++/##𝑃<0.01; ^***/+++/###𝑃<0.001.

(a) Representative gel image

(b) PNMT intron-retaining mRNA

(c) PNMT intronless mRNA

(d) Intracellular ROS levels

(e) NO concentration