Atherogenesis May Involve the Prooxidant and Proinflammatory Effects of Ferryl Hemoglobin
Figure 2
FerrylHb triggers EC death via different mechanisms. (a) Confluent HUVECs grown on 96-well plates were exposed to heme, Hb, metHb, and ferrylHb (5 μmol/L heme) in HBSS for 1 hour. After washing with HBSS, cells were challenged with H2O2 (75 μmol/L in HBSS) for 4 hours, followed by MTT assay to assess cell viability. (b) LDL was incubated with heme, Hb, metHb, and ferrylHb (10 μmol/L heme) overnight. HUVECs were exposed to LDL samples at a dose of 250 μg protein/mL for 4 hours. MTT assay was performed to determine cell viability. Results are shown as mean ± S.D. () from one representative experiment of three.
