Inositol Alleviates Pulmonary Fibrosis by Promoting Autophagy via Inhibiting the HIF-1α-SLUG Axis in Acute Respiratory Distress Syndrome

Figure 5

SLUG was a direct target of HIF-1α. (a) An HIF-1α binding site at loci 776-783 in the promoter region of SLUG was predicted by JASPAR. A dual luciferase reporter assay was performed to detect the regulation of SLUG by HIF-1α. Data represent the mean , , pcDNA-SLUG-WT+HIF-1α group vs. pcDNA-SLUG-WT group: . (b) The binding function of HIF-1α to SLUG was analyzed by ChIP-PCR analysis; inositol served as a positive control, and IgG served as a negative control. (c, d) Colocalization of SLUG and HIF-1α in lung tissues and cells as detected by IF staining. (e) SLUG, HIF-1α, N-cadherin, and E-cadherin expression in cells with HIF-1α overexpression was detected by qPCR. (f) HIF-1α, SLUG, E-cadherin, and N-cadherin expression was detected by western blotting. Data represent the mean , , over-HIF-1α vs. vector: .

(a)

(b)

(c)

(d)

(e)

(f)