Synergistic Cytotoxicity of Extracts of Chaga Mushroom and Microalgae against Mammalian Cancer Cells In Vitro

Figure 5

Synergistic effects of I. obliquus and microalgae extracts on mammalian cancer cells. Cancer cells were treated with MH-E1-SF or GLE1-CF-SF extracts (see Table 1) alone and in combination for 24 hr. (a) Synergy and antagonism space, as mapped by the highest single agent (HSA) model of synergy, expressed as a percentage of control. The level of synergism (blue) or antagonism (red) at each concentration is represented by color scale bar. (b) Experimental two extract combination dose–response surface, expressed as a percentage of the control value. The level of synergism (blue) or antagonism (red) at each concentration is represented by the color scale bar. All experiments were conducted at least three times. (c) The synergy of each combination was assessed using the combination index from the isobologram analysis (Loewe model of synergy). A combination index with less than 1 shows synergy, equal to 1 shows an additive effect, and greater than 1 shows an antagonistic effect.

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