Research Article
Synergistic Cytotoxicity of Extracts of Chaga Mushroom and Microalgae against Mammalian Cancer Cells In Vitro
Table 1
Extraction methods and total yield of the extracts of I. obliquus and microalgae.
| | Extracts | Extraction method | Starting quantity (g) | Final yield (mg) | Yield (%) |
| | I. obliquus | | MH-E1 | Ethanol (ultrasonication) | 10 | 336 | 3.4 | | MH-E1-SF | Ethanol sugar-free (chromatography) | 30 | 804 | 2.68 | | MH-H2O | Hot water | 25 | 3,700 | 14.8 | | MH-SFE1b | Supercritical (9,000 psi, 50°C, 1 hr) | 40 | 41 | 0.1 | | MH-SFE1c | Supercritical (7,500 psi, 50°C, 10% ethanol, 1 hr) | 40 | 237 | 0.59 | | Microalgae | | GLE1 | Ethanol (ultrasonication) | 30 | 1,442 | 4.8 | | GLE1-CF | Ethanol (ultrasonication; chlorophyll-free) | 2.5 | 270 | 10.8 | | GLE1-CF-SF | Ethanol (ultrasonication; chlorophyll- and sugar-free) | 2.5 | 176.6 | 7.06 |
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