Synergistic Cytotoxicity of Extracts of Chaga Mushroom and Microalgae against Mammalian Cancer Cells In Vitro

Table 4

Combination index (CI) from isobologram analysis of selected extracts of I. obliquus and microalgae with various ratios.

Cells	Ratios of the two extracts
	MH : GL (1 : 1)	MH : GL (2 : 1)	MH : GL (1 : 2)	MH : GL (4 : 1)	MH : GL (1 : 4)
MCF7	1.27	1.15	1.15	0.42	0.41
HepG2	0.76	0.76	0.78	0.43	0.40
HOS	1.27	0.95	0.99	0.42	0.46
D-17	1.17	0.84	0.76	0.47	0.47
DH-82	1.17	0.80	0.82	0.49	0.45

MH, MH-E1-SF (sugar-free ethanol extract of I. obliquus), GL, GLE1-CF-SF (chlorophyll- and sugar-free ethanol extract of microalgae mixture); MCF-7, human breast epithelial adenocarcinoma; HepG2, human hepatocellular carcinoma; HOS, human osteosarcoma; D-17, canine osteosarcoma; and DH-82, canine histiocytosis. CI < 1: synergism, CI = 1: additive effect, and CI > 1: antagonism.