Research Article
Determination of Platycodin D and Platycodin D3 in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry
Table 4
Stability of PD in rat plasma (
at each level).
| Conditions | Theoretical concentration (ng/mL) | Final concentration (ng/mL) | RE (%) | CV (%) |
| Three freeze/thaw cycles (−70°C) | 150 | 149.8 | 0.1 | 4.2 | 1000 | 1005.3 | 0.5 | 3.7 | 8000 | 7956.8 | −0.5 | 3.2 |
| Bench top stability (room temp., 24 h) | 150 | 147.9 | −1.4 | 7.0 | 1000 | 1053.5 | 5.4 | 5.2 | 8000 | 8180.9 | 2.3 | 1.8 |
| Postpreparative stability (4°C, 24 h) | 150 | 147.0 | −2.0 | 6.2 | 1000 | 927.1 | −7.3 | 1.1 | 8000 | 7479.3 | −6.5 | 1.3 |
| Long term stability (−70°C, 1 week) | 150 | 148.4 | −1.1 | 5.5 | 1000 | 973.9 | −2.6 | 3.1 | 8000 | 7425.3 | −7.2 | 1.9 |
| Long term stability (−70°C, 2 weeks) | 150 | 152.2 | 1.5 | 3.7 | 1000 | 993.3 | −0.7 | 6.1 | 8000 | 7477.6 | −6.5 | 3.6 |
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CV: coefficient of variation; RE: relative error.
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