Bridging the Divide between Manual Gating and Bioinformatics with the Bioconductor Package flowFlowJo
Figure 3
Comparison of the monocyte P-p38 mean fluorescent intensity as determined by the different gating methods for the 96 samples of whole blood. The apparent P-p38 response in any particular sample may be affected by the donor, the person running the assay, and the amount of TNF- stimulation applied to the cells. The points corresponding to the two samples shown in Figure 2 are labeled in red.
