In Silico Analysis of Gene Expression Network Components Underlying Pigmentation Phenotypes in the Python Identified Evolutionarily Conserved Clusters of Transcription Factor Binding Sites

Figure 11

MLANA, RAB27A, and MC1R upstream regions and associated candidate enhancer modules. (a) The upstream region from melan-A (MLANA) contains a cluster of binding sites beginning at position 275 and ending at position 475 which include recognition sites for LEF-1, POU3F2, USF-1, and FOX3D. A second cluster of binding sites begins at position 650 and extends to position 850 which contains binding sites for POU3F2, LEF-1, and FOX3D. The third cluster of sites occurs between position 1100 and position 1300 which contains binding sites for FOXD3, POU3F2, and USF-1. (b) The upstream region for RAS oncogene family member 27A (RAB27A) (Figure 11(b)) has a cluster of transcription factor binding sites beginning at position 250 and ending at position 450 which include sites for POU3F2, USF-1, and FOXD3. The second cluster of binding sites begins at position 500 and ends at position 700 which contains sites for POU3F2, USF-1, FOX3D, and LEF-1. The third cluster begins at position 1050 and ends at position 1250 and binds LEF-1, USF-1, CREB, FOX3D, and POU3F2. (c) The upstream region for melanocortin 1 receptor (MC1R) contains a cluster of transcription factor binding sites for USF-1 and POU3F2 between position 150 and position 350. A second cluster begins at position 725 and ends at position 925 with binding sites for USF-1, LEF-1, FOX3D, POU3F2, and MITF. A third cluster of binding sites is located between position 1225 and position 1425 with sites for LEF-1, USF-1, POU3F2, and FOXD3.

(a)

(b)

(c)