Pigment Melanin Scavenges Nitric Oxide In Vitro: Possible Relevance to Keloid Formation

Figure 3

(a) Determination of NO as NO₂₋ by Griess analysis in 0.1 M phosphate buffer, pH 7.4. Black circles: initial amount of NO=1.44 nmole in “melanin bag” (see text). White circles: initial amount of 1.44 nmole in the absence of melanin (“blank bag”; see text). Mean±S.D. of 3 determinations, 𝑃<.03. (b) Determination of NO as NO₃₋ by Griess analysis in 0.1 M phosphate buffer, pH 7.4 under the same conditions as in Figure 3(a). Black circles: “melanin bag.” White circles: “blank bag”; see text. Mean±S.D. of 3 determinations, 𝑃<.02.

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(b)