Origin and Regenerative Potential of Vertebrate Mechanoreceptor-Associated Stem Cells

Figure 3

Cultivated palatal neural crest-derived stem cells form self-adherent neurospheres and express the intermediate filament Nestin. Human palatal NCSCs were isolated according to protocol described in [3], fixed using 4% paraformaldehyde and stained using primary antibody against human Nestin and Alexa 555-coupled secondary antibody. DNA was stained using SYTOX green (bar 20 𝜇m).