- About this Journal
- Abstracting and Indexing
- Aims and Scope
- Article Processing Charges
- Articles in Press
- Author Guidelines
- Bibliographic Information
- Citations to this Journal
- Contact Information
- Editorial Board
- Editorial Workflow
- Free eTOC Alerts
- Publication Ethics
- Reviewers Acknowledgment
- Submit a Manuscript
- Subscription Information
- Table of Contents
Anatomy Research International
Volume 2012 (2012), Article ID 837626, 9 pages
Origin and Regenerative Potential of Vertebrate Mechanoreceptor-Associated Stem Cells
1Department of Cell Biology, University of Bielefeld, Universitätsstraße 25, 33501 Bielefeld, Germany
2Department of Molecular Neurobiology, University of Bielefeld, Universitätsstraße 25, 33501 Bielefeld, Germany
Received 9 July 2012; Accepted 4 September 2012
Academic Editor: David Yew
Copyright © 2012 Darius Widera et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Meissner corpuscles and Merkel cell neurite complexes are highly specialized mechanoreceptors present in the hairy and glabrous skin, as well as in different types of mucosa. Several reports suggest that after injury, such as after nerve crush, freeze injury, or dissection of the nerve, they are able to regenerate, particularly including reinnervation and repopulation of the mechanoreceptors by Schwann cells. However, little is known about mammalian cells responsible for these regenerative processes. Here we review cellular origin of this plasticity in the light of newly described adult neural crest-derived stem cell populations. We also discuss further potential multipotent stem cell populations with the ability to regenerate disrupted innervation and to functionally recover the mechanoreceptors. These capabilities are discussed as in context to cellularly reprogrammed Schwann cells and tissue resident adult mesenchymal stem cells.
Meissner corpuscles (MCs, also called tactile corpuscles) were first described in 1852 by the German physiologists Rudolf Wagner and Georg Meissner . These are encapsulated, rapidly adapting mechanoreceptors responsible for sensing light touch on the skin. Recently, due to their immunocytochemical properties, it has been proposed that MC may also act as nociceptors . They can be found within the dermis, beneath the basal layer of skin regions sensitive to light touch. Within the murine, rat and human palatal mucosa, MCs are located centrally within palatal ridges (rugae palatinae) and are often accompanied by Merkel cell-neurites  (see Figure 1(a)). Remarkably, an anterior-posterior gradient of Nestin-expressing cells within the rat palate could be identified (see Figure 2). In particular, numerous Nestin-positive MCs can be observed in the lamina propria of hard palate, whereas nearly no MCs are present in the soft palate. In humans, the number of MCs gradually decreases with age .
A further type of highly specialized mechanoreceptors is the Merkel cell-neurite complexes (Merkel nerve endings), which are, in contrast to Meissner corpuscles, not encapsulated and seem to act as slowly adapting mechanoreceptors responsible for sustained sensing of mechanical pressure. In mammals, they are widely distributed and can be found in the basal layer of the palatal/oral mucosa (see Figure 1(a)), as well as in hairy and glabrous skin. Merkel cells were first described in 1875 by Friedrich Siegmund Merkel  and were originally termed “Tastzellen” (German: touch cells) (reviewed in ).
Remarkably, after injury, such as experimental nerve crash or freeze injury, MCs and Merkel cells seem to harbor a limited capacity to regenerate [7–9]. However, the exact cellular and developmental origin of plastic cells within MCs and Merkel cell-neurites responsible for this limited plasticity remains unclear. Recently, we demonstrated high expression of neural crest and general stem cell markers as well as pluripotency-associated transcripts within rat palatal mucosa. In addition, immunocytochemical analysis revealed high expression of Nestin within the MCs and adjacent to Merkel cell-neurite complexes, suggesting the presence of stem cells or other cells with progenitor properties within these mechanoreceptors .
In the following, we review the cellular composition of Meissner corpuscles and Merkel cell-neurite complexes. We focus on their developmental ancestry, marker expression, and the potential origin of multipotent stem cells within these highly specialized mechanoreceptors, factors which might explain their regenerative potential.
2. Cellular Composition of Meissner Corpuscles and Merkel Cell-Neurite Complexes
At the cellular level, MCs consist of a coiled arrangement of endings from up to six myelinated axons that terminate between layers of very thin, flattened, specialized Schwann cells called lamellar cells (see schematic diagram in Figure 4) [10–13]. MCs are connected with thin axons and contain the myelinated axons as well as several unmyelinated nerve fibers [10, 14].
MCs contain several fibroblastoid cells, generating the connective tissue capsule. This capsule is distinguishable from the surrounding tissue, since this extracellular matrix is mainly of fibrillar connective tissue consisting of fibrillary proteins like collagens and fibronectins. Importantly, lamellar cells itself are embedded within the so-called basal lamina consisting among others of laminin and collagen type IV (reviewed in ). Notably, the extracellular matrix ECM, including the basal lamina, plays an essential role in connection, support, and growth regulation of cells in vivo and may therefore have major impact on cells responsible for regenerative processes. This may be due to direct influence on differentiation and proliferation or due to guidance of cells migrating into lesioned MCs.
However, although the acellular components and the cellular composition of MCs have been studied intensely using morphological and immunocytochemical analyses, the existence of further cell types like adult neural crest-derived stem cells or tissue resident mesenchymal stem cells has not been previously reported.
Merkel cell-neurite complexes consist of large, oval Merkel cells (Merkel-Ranvier Cells), which are in synapse-like contact with flat terminal endings of myelinated nerve fibers. In addition to their postulated function as receptor cells essential for tactile discrimination, Merkel cells may also exhibit an endocrine function, as they contain dense core granules comprising various neuropeptides [16–18]. In addition, due to the close spatial proximity of Merkel cells to associated myelinated nerve fibres, several myelinating Schwann cells are known to be located adjacent to Merkel cell-neurite complexes.
3. Expression Pattern
Within MCs, neuronal cells can be identified based on their characteristic expression of neuronal markers including neurofilaments, -III-tubulin, synaptophysin, protein gene product 9.5 (PGP 9.5) , and neuron-specific enolase (NSE) [3, 13, 20, 21]. In addition, MCs expressed a panel of specific ion channels (reviewed in ). Nonneuronal cells of MC show robust expression of S100, Nestin (Figure 1(b)), Vimentin as well as the epidermal growth factor receptor (EGFR) [3, 20, 21, 23]. Several studies reported strong immunoreactivity for the neurotrophin receptors TrkA, TrkB, and p75 NTR [20, 21, 24]. Human MCs are negative for cytokeratins and GFAP, as described by Vega and colleagues . Very recently, Meissner corpuscles have been described to express acid-sensing ion channels 2 (ASIC2) .
Merkel cells are characterized by the expression of cytokeratins CK8, CK18, CK19, and CK20 (reviewed in ) and the absence of CK4 and CK13 . CK20 is of note as the most specific marker of Merkel cells; its expression shows no overlap with other cell types. Moreover, Merkel cells show expression of p75NTR, whereas no expression of Vimentin or GFAP can be detected by immunocytochemistry [27, 28]. Although we detected Nestin-positive cells in close proximity to Merkel cell-neurite complexes (Figure 1(b)), there are no direct evidences that Merkel cells itself are positive for this intermediate filament . Indeed, a study by Eispert suggested that Nestin is absent in human Merkel cells of different anatomical origin . Merkel cells are known to express a variety of neuronal markers like NSE, Synaptophysin, and PGP 9.5, whereas Neurofilament is absent [26, 28, 30–33]. Additionally, it was reported that Neurotrophin-3 (NT-3) is required for the development of Merkel cells . Recently, it has been reported that CK20-positive Merkel cells within human skin express Sox2—a pluripotency-associated transcription factor which is crucial for embryonic development as well as embryonic and adult neural crest-derived stem cell populations .
4. Developmental Origin of Meissner Corpuscles and Merkel Cell-Neurite Complexes
It has been suggested that in addition to the Schwann cell-related cells of the MC, the nerve fibres within NC are also of neural crest origin . In this study, the authors used lineage tracing in Wnt1Cre/RetfCFP mice and demonstrated expression of CFP in neuronal cells, thus elucidating their neural crest ancestry. However, the discussion surrounding the developmental origin of Merkel cells is controversial. In the avian system, it has been suggested that Merkel cells also arise from the neural crest . To address the question of the ontogenic origin of mammalian Merkel cells, Maya Sieber-Blums and colleagues used Wnt1Cre/Rosa26R reporter mice in which cells of neural crest lineage can be clearly traced back via expression of -galactosidase (-gal) . In this study, CK8-immunopositive Merkel cells showed clear coexpression of -gal, suggesting their neural-crest origin. In addition, electron microscopy after peroxidase immunostaining revealed presence of -gal and Merkel cell typical dense core granules. A contrary model for the developmental origin of mammalian cells was proposed in 2009 by deletion of Atoh1 in either epidermal (Krt14Cre ) or neural crest deleter mice (Wnt1Cre ). In this approach, the authors selectively deleted Atoh1, which is necessary for the survival of Merkel cells, in epidermal, Krt14-expressing cells or in Wnt1-positive progeny of the neural crest. Interestingly, epidermal deletion of Atoh1 resulted in loss of Merkel cells in the skin, whereas neural crest-specific deletion showed no significant effects . As the debate surrounding these findings continues, the final assessment of the developmental origin of Merkel cells requires further experimental analysis and as the exact nature of this origin remains elusive.
5. Regenerative Potential of MCs and Merkel Cell-Neurite Complexes
In the last two decades of the twentieth century, several reports provided evidences for regenerative capacity of MCs and Merkel cell-neurite complexes [7–9, 40–42]. Through transection of the nerve innervating Meissner corpuscles in mice, Chizuka Ide observed that after an initial degeneration, at least some corpuscles start to regenerate, including re-innervation 30 days after the section and proper morphological appearance 4 months after the surgery . After freezing injury, complete disintegration of cells within the corpuscles occurs after 25 days. In an advanced experimental procedure involving freezing damage, it was observed that the regenerated axons demonstrated extensive branching and morphologically specialized axonal terminals as well as the occurrence of newly formed lamellar cells . Here, the author postulated that Schwann cells migrated into the site of the lesion and differentiated into lamellar cells, although the ancestry of the regenerated axon was not investigated further. Studies by Zelena and colleagues revealed that the regeneration potential of Meissner corpuscles after nerve crush and experimental freeze injury is higher in older animals than in young rats [8, 9].
In addition to MCs, Merkel cells as well seem to regenerate after injury. In an intriguing initial study, reinnervation and an increase of the Merkel cell number after experimental nerve crush were observed 40–100 days after the surgery [7, 43]. Importantly, the authors were able to show that the newly formed Merkel cells appeared physiologically normal and demonstrated typical histological features via electron microscopy and toluidine blue staining.
Although these reports clearly suggested that mechanoreceptors such as MCs and Merkel cell-neurite complexes can regenerate at least to a certain degree, the exact cellular origin of such unexpected plasticity remains unclear. Thus, the existence of so far unknown plastic progenitors or stem cells responsible for the regeneration process seems likely and is further discussed here.
6. Potential Stem Cell/Progenitor Populations within MCs and Merkel Cell-Neurite Complexes
6.1. Mammalian Adult Neural Crest-Derived Stem Cells
In general, such neural crest-derived stem cells (NCSCs) are pluripotent in the early embryonic development (able to differentiate into cells of all germ layers) and become more restricted after migration from their niche between the ectoderm and the neural tube. A limited number of NCSCs that persist in the adult are able to generate multiple cell progenies in vitro and in vivo and aretherefore, multipotent stem cells (reviewed in ). In particular, such cells have been described to efficiently differentiate into neuronal and glial cells, osteogenic cell types, adipocytes, and chondrocytes, as well as into melanocytes and muscle cells. Such adult NCSCs express in vitro and in vivo high levels of Nestin, which is an intermediate filament originally described in Schwann cells and important for the self-renewal of neural stem cells [53, 54]. Adult NCSCs show expression of Vimentin, Sox2, and, depending on the cultivation method, the neurotrophin receptor p75NTR. As observed in adult human NCSCs isolated from respiratory mucosa, such cells also express TrkA (Hauser et al., unpublished observation). In addition, their expression pattern further includes Sox9, Sox10, Klf4, c-Myc, and Oct4 (see  for the full list). When cultivated under serum-free conditions in vitro, NCSCs form self-adherent clusters similar to neural stem cell-derived neurospheres.
In 2009, we reported Nestin-expressing NCSCs adjacent to MCs and Merkel cell-neurite complexes within the palatal ridges (palatal rugae/rugae palatinae) of adult rats . Due to their anatomical origin within the palate, we termed these palatal neural crest-derived stem cells (pNCSCs). In their niche, the palatal ridges of hard palate, pNCSCs demonstrated high expression of Nestin, as demonstrated by immunocytochemical analysis and RT-PCR. In particular, Nestin immunoreactivity was detected in the periphery, centre, and on the top of Meissner corpuscles, as well as in close proximity to Merkel cell-neurite complexes within the basal layer of the palatal mucosa. We were further able to successfully isolate and expand rat and human palatal NCSCs in vitro as free-floating neurosphere cultures [3, 21] (see also Figure 3). Such cultivated pNCSCs were positive for a set of stem cell markers including Nestin, p75NTR, Sox9, Notch1, Slug and Snail in addition to Sox2, Klf4, Oct4, and c-Myc. Using appropriate differentiation protocols we demonstrated that pNCSCs were not only able to differentiate into GFAP-expressing glial cells, but also into -III-tubulin, NF- and Map2-positive neuronal cells. Due to such tremendous plasticity, tissue-resistant NCSCs are strong candidates for the observed in vivo regeneration of MCs and Merkel cell-neurite complexes after dissection and consequent degeneration. However, at least in case of regeneration after freeze injury, in which all cells within the mechanoreceptors including adjacent cells perish, there must be a further source of plastic cells capable of migration to the site of the lesion. These may be other NCSCs attracted by injury signals (e.g., chemokines and growth factors) from greater spatial distances or different cell types like cellularly reprogrammed Schwann cells or their progenitors (see the following).
6.2. Schwann Cell Progenitors and Dedifferentiated Schwann Cells
Schwann cells are directly related to the neural crest and share several markers with NCSCs (e.g., Vimentin, Nestin, or p75NTR, reviewed in ). Schwann cell progenitors (SCPs) are considered as late NCSCs that have established contact with axons . It has been proposed that Schwann cell progenitors and Schwann cells have an extraordinarily unstable phenotype as differentiated Schwann cells can be switched back into more primitive cell types by injury signals or by appropriate cultivation methods [56, 57]. Consequently, these data suggest that Schwann cells and their immature ancestors—Schwann cell progenitors—may act as stem cells. In accordance with this hypothesis, Schwann cells have been described to perform differentiation into melanocytes after the lesion of the adult sciatic nerve [58, 59]. After injury, Schwann cells can reenter the cell cycle and dedifferentiate [56, 60]. Dupin and colleagues demonstrated differentiation of Schwann cells into myofibroblasts, indicating stemness characteristics of such cells in vitro .
Recently, we demonstrated successful cellular reprogramming of adult myelinating Schwann cells into an immature multipotent NCSC phenotype . After isolation and expansion of Schwann cells under culture conditions mimicking an in vivo injury, we observed significantly elevated expression levels of p75, c-Myc, Sox2, Klf4, Oct4, Sox9, and Slug. Importantly, we were also able to differentiate such cultivated adult Schwann cells into ectodermal and mesodermal progeny. Such cellular reprogramming into immature neural crest-like phenotype also seems to occur in vivo in response to injury. This has been impressively demonstrated in a Wnt Cre/lox-EGFP mouse model . After injury, mature Schwann cells residing at the nerve roots dedifferentiate into proliferating p75NTR-positive immature Schwann cells, which migrate into the lesion site.
It might be assumed that a similar injury-induced reprogramming mechanism could switch mechanoreceptor-associated Schwann cells, such as lamellar cells of MCs, into more primitive phenotype. Since mechanoreceptor-associated, subcutaneous Schwann cells are permanently exposed to mild mechanical stress, a cellular turnover and a latent cellular plasticity may be a hallmark of those cells. Indeed, in our study we detected proliferating, Ki67-positive cells not only in the basal cell layer of the palatal mucosa, but also in the center of MCs . The expression of p75NTR is usually observed in immature, plastic Schwann cell progenitors. However, we and others reported that lamellar cells within MCs show strong p75NTR-immunoreactivity in addition to the well-described expression of S100 [20, 21]. Within palatal MCs, S100-immunoreactivity was detected in 100% of the cells in the investigated region, whereas not all cells expressed p75NTR (~50%) . In addition, we observed a high degree of co-expression between Nestin and p75NTR (nearly 100%). Such co-expression of Nestin and p75NTR is a typical marker of immature cells such as early Schwann cell progenitors. However, the most likely source of multipotent neural crest-related stem cells within palatal MCs may be myelinating Schwann cells and not Schwann cell progenitors. Although SCPs are defined as neural crest stem cells which have established contact to nerve fibers, myelinization seems to be crucial for Nestin expression as Nestin has always been observed within myelinating Schwann cells and not in Schwann cells directly in contact with unmyelinated axons .
6.3. Tissue Resident Mesenchymal Stem Cells as Further Putative Stem Cell Type Responsible for Regeneration
A further adult stem cell type is represented by mesenchymal stem cells. Beside their initially described niche within the mammalian bone marrow, the presence of adult MSCs has also been confirmed in almost all tissues including muscle, fat, and the dermis of diverse types of mucosa . Similar to adult NCSCs, MSCs are able to generate osteogenic, adipogenic, and chondrogenic progeny, although their neuronal differentiation potential is a subject of an ongoing scientific debate [63–65]. At least in vitro, MSCs seem to show the ability to differentiate into neuron-like and Schwann cell-like phenotypes [66, 67] (reviewed in ). Concerning their morphological and immunocytochemical properties, as well as the expression profile, MSCs show high degree of similarity to fibroblasts, making them nearly indistinguishable in vivo [68–71]. Importantly, in addition to typical MSC markers such as CD105, CD73 and CD90, MSCs express also Nestin and Vimentin [64, 72, 73].
As discussed previously, MCs contain not only glial cell, such as lamellar cells, but also fibroblast-like cells which, in the classical view, generate the connective encapsulation of the corpuscle. Remarkably, we observed Nestin immunoreactivity not only in lamellar cells of MCs, but also adjacent to the connective encapsulation of the corpuscles [3, 21]. Therefore, it cannot be excluded that MCs and adjacency of Merkel cell-neurite complexes contain both at least some tissue resident MSCs.
Taking into consideration that MSCs may be able to differentiate into multiple progenies, also this cell type can address the question of mechanoreceptor regeneration.
In this context, it is noteworthy that in our studies, we focused on cranial mucosa, a tissue where also tissue-resident MSCs are clearly of neural crest origin . Thus, such cranial neural crest-derived MSCs may reveal a higher degree of plasticity than their trunk counterparts, which are not of neural crest ancestry .
As summarized previously, there are different multipotent cell populations in anatomical adjacency of mechanoreceptors which may be responsible for regenerative processes after lesion (see Figure 4). Firstly, due to their extraordinarily high plasticity, NCSCs may directly contribute to the regeneration process in vivo. A second plastic cell population is represented by cellularly reprogrammed Schwann cells, which reenter the cell cycle and dedifferentiate into more primitive phenotypes after lesion in their endogenous niche. Tissue resident MSCs may also play an important role in recovery after degeneration of mechanoreceptors. Additionally, a cooperative manner action, including more than one cell type, cannot be excluded at this time.
A definitive identification of the cellular base of regeneration remains a future challenge, the elucidation of which may help to develop effective treatments for acute injuries and age-related diseases accompanied by loss of mechanoreceptors, such as peripheral neuropathies. In vitro cultivation of autologous, mechanoreceptor-associated stem cells may be the basis of new therapeutic strategies.
Conflict of Interests
The authors declare no potential conflict of interests.
Experimental work described herein that was performed in our laboratory was supported by a grant of University of Bielefeld to D. Widera, grants of the German Research Council (DFG) to C. Kaltschmidt, and a grant of the German Ministry of Research and Education (BMBF) to B. Kaltschmidt. The authors thank Johannes FW Greiner and Kyle J. Lauersen for critical reading. They acknowledge support for the Article Processing Charge by the Deutsche Forschungsgemeinschaft and the Open Access Publication Funds of Bielefeld University Library.
- R. Wagner and G. Meissner, Ueber das Vorhandensein bisher unbekannter eigenthümlicher Tastkörperchen (Corpuscula tactus) in den Gefühlswärzchen der menschlichen Haut und über die Endausbreitung sensitiver Nerven, 1852.
- M. Paré, R. Elde, J. E. Mazurkiewicz, A. M. Smith, and F. L. Rice, “The meissner corpuscle revised: a multiafferented mechanoreceptor with nociceptor immunochemical properties,” Journal of Neuroscience, vol. 21, no. 18, pp. 7236–7246, 2001.
- D. Widera, C. Zander, M. Heidbreder et al., “Adult palatum as a novel source of neural crest-related stem cells,” Stem Cells, vol. 27, no. 8, pp. 1899–1910, 2009.
- K. Schimrigk and H. Ruettinger, “The touch corpuscles of the plantar surface of the big toe. histological and histometrical investigations with respect to age,” European Neurology, vol. 19, no. 1, pp. 49–60, 1980.
- F. Merkel, “Tastzellen und Tastkörperchen bei den Hausthieren und beim Menschen,” Archiv für Mikroskopische Anatomie, vol. 11, no. 1, pp. 636–652, 1875.
- Z. Halata, M. Grim, and K. I. Bauman, “Friedrich Sigmund Merkel and his “Merkel cell”, morphology, development, and physiology: review and new results,” Anatomical Record, vol. 271, no. 1, pp. 225–239, 2003.
- C. A. Nurse, L. Macintyre, and J. Diamond, “Reinnervation of the rat touch dome restores the Merkel cell population reduced after denervation,” Neuroscience, vol. 13, no. 2, pp. 563–571, 1984.
- J. Zelena, I. Jirmanova, T. Nitatori, and C. Ide, “Effacement and regeneration of tactile lamellar corpuscles of rat after postnatal nerve crush,” Neuroscience, vol. 39, no. 2, pp. 513–522, 1990.
- I. Jirmanová, P. Dubový, and J. Zelená, “Regeneration of tactile lamellar corpuscles of the rat after postnatal freeze injury,” Anatomy and Embryology, vol. 195, no. 4, pp. 363–374, 1997.
- N. Cauna, “Nerve supply and nerve endings in Meissner's corpuscles,” The American Journal of Anatomy, vol. 99, no. 2, pp. 315–350, 1956.
- Z. Halata, “The mechanoreceptors of the mammalian skin ultrastructure and morphological classification,” Advances in Anatomy, Embryology, and Cell Biology, vol. 50, no. 5, pp. 3–77, 1975.
- B. L. Munger and C. Ide, “The structure and function of cutaneous sensory receptors,” Archives of Histology and Cytology, vol. 51, no. 1, pp. 1–34, 1988.
- D. Guinard, Y. Usson, C. Guillermet, and R. Saxod, “PS-100 and NF 70-200 double immunolabeling for human digital skin meissner corpuscle 3D imaging,” Journal of Histochemistry and Cytochemistry, vol. 48, no. 2, pp. 295–302, 2000.
- Dogiel, “Nervenendigungen in Meissnerschen Tastkörperchen,” Monthly International Journal of Anatomy and Physiology, vol. 9, pp. 76–85, 1892.
- J. A. Vega, O. García-Suárez, J. A. Montaño, B. Pardo, and J. M. Cobo, “The Meissner and pacinian sensory corpuscles revisited new data from the last decade,” Microscopy Research and Technique, vol. 72, no. 4, pp. 299–309, 2009.
- S. M. Maricich, S. A. Wellnitz, A. M. Nelson et al., “Merkel cells are essential for light-touch responses,” Science, vol. 324, no. 5934, pp. 1580–1582, 2009.
- S. Polakovicova, H. Seidenberg, R. Mikusova, S. Polak, and V. Pospisilova, “Merkel cells—review on developmental, functional and clinical aspects,” Bratislavské Lekárske Listy, vol. 112, no. 2, pp. 80–87, 2011.
- A. Lucarz and G. Brand, “Current considerations about merkel cells,” European Journal of Cell Biology, vol. 86, no. 5, pp. 243–251, 2007.
- O. García-Suárez, J. A. Montaño, I. Esteban et al., “Myelin basic protein-positive nerve fibres in human Meissner corpuscles,” Journal of Anatomy, vol. 214, no. 6, pp. 888–893, 2009.
- J. A. Vega, J. J. Haro, and M. E. del Valle, “Immunohistochemistry of human cutaneous Meissner and pacinian corpuscles,” Microscopy Research and Technique, vol. 34, no. 4, pp. 351–361, 1996.
- D. Widera, P. Heimann, C. Zander, Y. Imielski, M. Heidbreder, et al., “Schwann cells can be reprogrammed to multipotency by culture,” Stem Cells and Development, vol. 20, no. 12, pp. 2053–2064, 2011.
- M. E. Valle, T. Cobo, J. L. Cobo, and J. A. Vega, “Mechanosensory neurons, cutaneous mechanoreceptors, and putative mechanoproteins,” Microscopy Research and Technique, vol. 75, no. 8, pp. 1033–1043, 2012.
- J. A. Vega, E. Vazquez, F. J. Naves, B. Calzada, M. E. del Valle, and J. J. Represa, “Expression of epidermal growth factor receptor (EGFr) immunoreactivity in human cutaneous nerves and sensory corpuscles,” Anatomical Record, vol. 240, no. 1, pp. 125–130, 1994.
- M. G. Calavia, J. Feito, L. López-Iglesias et al., “The lamellar cells in human Meissner corpuscles express TrkB,” Neuroscience Letters, vol. 468, no. 2, pp. 106–109, 2010.
- R. Cabo, M. A. Galvez, I. San Jose, A. Lopez-Muniz, I. San Jose, et al., “Immunohistochemical localization of acid-sensing ion channel 2 (ASIC2) in cutaneous Meissner and pacinian corpuscles of Macaca fascicularis,” Neuroscience Letters, vol. 516, no. 2, pp. 197–201, 2012.
- I. Moll, R. Paus, and R. Moll, “Merkel cells in mouse skin: intermediate filament pattern, localization, and hair cycle-dependent density,” Journal of Investigative Dermatology, vol. 106, no. 2, pp. 281–286, 1996.
- I. Kinkelin, C. L. Stucky, and M. Koltzenburg, “Postnatal loss of Merkel cells, but not of slowly adapting mechanoreceptors in mice lacking the neurotrophin receptor p75,” European Journal of Neuroscience, vol. 11, no. 11, pp. 3963–3969, 1999.
- J. H. Saurat, L. Didierjean, O. Skalli, G. Siegenthaler, and G. Gabbiani, “The intermediate filament proteins of rabbit normal epidermal Merkel cells are cytokeratins,” Journal of Investigative Dermatology, vol. 83, no. 6, pp. 431–435, 1984.
- A. C. Eispert, F. Fuchs, J. M. Brandner, P. Houdek, E. Wladykowski, and I. Moll, “Evidence for distinct populations of human Merkel cells,” Histochemistry and Cell Biology, vol. 132, no. 1, pp. 83–93, 2009.
- G. Zaccone, “Neuron-specific enolase and serotonin in the Merkel cells of conger-eel (Conger conger) epidermis. An immunohistochemical study,” Histochemistry, vol. 85, no. 1, pp. 29–34, 1986.
- W. Hartschuh and E. Weihe, “Multiple messenger candidates and marker substances in the mammalian Merkel cell-axon complex: a light and electron microscopic immunohistochemical study,” Progress in Brain Research, vol. 74, pp. 181–187, 1988.
- C. J. Dalsgaard, M. Rydh, and A. Haegerstrand, “Cutaneous innervation in man visualized with protein gene product 9.5 (PGP 9.5) antibodies,” Histochemistry, vol. 92, no. 5, pp. 385–390, 1989.
- R. Gallego, T. Garcia-Caballero, M. Fraga, A. Beiras, and J. Forteza, “Neural cell adhesion molecule immunoreactivity in Merkel cells and Merkel cell tumours,” Virchows Archiv, vol. 426, no. 3, pp. 317–321, 1995.
- M. S. Airaksinen, M. Koltzenburg, G. R. Lewin et al., “Specific subtypes of cutaneous mechanoreceptors require neurotrophin-3 following peripheral target innervation,” Neuron, vol. 16, no. 2, pp. 287–295, 1996.
- A. C. Laga, C. Y. Lai, Q. Zhan et al., “Expression of the embryonic stem cell transcription factor SOX2 in human skin: relevance to melanocyte and merkel cell biology,” American Journal of Pathology, vol. 176, no. 2, pp. 903–913, 2010.
- W. Luo, H. Enomoto, F. L. Rice, J. Milbrandt, and D. D. Ginty, “Molecular identification of rapidly adapting mechanoreceptors and their developmental dependence on ret signaling,” Neuron, vol. 64, no. 6, pp. 841–856, 2009.
- M. Grim and Z. Halata, “Developmental origin of avian Merkel cells,” Anatomy and Embryology, vol. 202, no. 5, pp. 401–410, 2000.
- V. Szeder, M. Grim, Z. Halata, and M. Sieber-Blum, “Neural crest origin of mammalian Merkel cells,” Developmental Biology, vol. 253, no. 2, pp. 258–263, 2003.
- K. M. Morrison, G. R. Miesegaes, E. A. Lumpkin, and S. M. Maricich, “Mammalian Merkel cells are descended from the epidermal lineage,” Developmental Biology, vol. 336, no. 1, pp. 76–83, 2009.
- C. Ide, “Regeneration of mouse digital corpuscles,” American Journal of Anatomy, vol. 163, no. 1, pp. 73–85, 1982.
- C. Ide, “Basal laminae and Meissner corpuscles regeneration,” Brain Research, vol. 384, no. 2, pp. 311–322, 1986.
- P. Dubovy and H. Aldskogius, “Degeneration and regeneration of cutaneous sensory nerve formations,” Microscopy Research and Technique, vol. 34, no. 4, pp. 362–375, 1996.
- M. Aimetti, F. Romano, L. Cricenti et al., “Merkel cells and permanent disesthesia in the oral mucosa after soft tissue grafts,” Journal of Cellular Physiology, vol. 224, no. 1, pp. 205–209, 2010.
- S. Yoshida, S. Shimmura, N. Nagoshi et al., “Isolation of multipotent neural crest-derived stem cells from the adult mouse cornea,” Stem Cells, vol. 24, no. 12, pp. 2714–2722, 2006.
- M. Sieber-Blum and M. Grim, “The adult hair follicle: cradle for pluripotent neural crest stem cells,” Birth Defects Research Part C, vol. 72, no. 2, pp. 162–172, 2004.
- R. Sasaki, S. Aoki, M. Yamato et al., “Neurosphere generation from dental pulp of adult rat incisor,” European Journal of Neuroscience, vol. 27, no. 3, pp. 538–548, 2008.
- N. Nagoshi, S. Shibata, Y. Kubota et al., “Ontogeny and multipotency of neural crest-derived stem cells in mouse bone marrow, dorsal root ganglia, and whisker pad,” Cell Stem Cell, vol. 2, no. 4, pp. 392–403, 2008.
- W. Techawattanawisal, K. Nakahama, M. Komaki, M. Abe, Y. Takagi, and I. Morita, “Isolation of multipotent stem cells from adult rat periodontal ligament by neurosphere-forming culture system,” Biochemical and Biophysical Research Communications, vol. 357, no. 4, pp. 917–923, 2007.
- D. Widera, W. D. Grimm, J. M. Moebius et al., “Highly efficient neural differentiation of human somatic stem cells, isolated by minimally invasive periodontal surgery,” Stem Cells and Development, vol. 16, no. 3, pp. 447–460, 2007.
- T. Nagase, D. Matsumoto, M. Nagase et al., “Neurospheres from human adipose tissue transplanted into cultured mouse embryos can contribute to craniofacial morphogenesis: a preliminary report,” Journal of Craniofacial Surgery, vol. 18, no. 1, pp. 49–53, 2007.
- S. Hauser, D. Widera, F. A. Qunneis, J. Mueller, C. Zander, et al., “Isolation of novel multipotent neural crest-derived stem cells from adult human inferior turbinate,” Stem Cells and Development, vol. 21, no. 5, pp. 742–756, 2011.
- B. Kaltschmidt, C. Kaltschmidt, and D. Widera, “Adult craniofacial stem cells: sources and relation to the neural crest,” Stem Cells, vol. 27, no. 8, pp. 1899–1910, 2009.
- B. Friedman, S. Zaremba, and S. Hockfield, “Monoclonal antibody rat 401 recognizes Schwann cells in mature and developing peripheral nerve,” Journal of Comparative Neurology, vol. 295, no. 1, pp. 43–51, 1990.
- D. Park, A. P. Xiang, F. F. Mao et al., “Nestin is required for the proper self-renewal of neural stem cells,” Stem Cells, vol. 28, no. 12, pp. 2162–2171, 2010.
- K. R. Jessen and R. Mirsky, “The origin and development of glial cells in peripheral nerves,” Nature Reviews Neuroscience, vol. 6, no. 9, pp. 671–682, 2005.
- E. Dupin, C. Real, C. Glavieux-Pardanaud, P. Vaigot, and N. M. Le Douarin, “Reversal of developmental restrictions in neural crest lineages: transition from Schwann cells to glial-melanocytic precursors in vitro,” Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 9, pp. 5229–5233, 2003.
- C. Real, C. Glavieux-Pardanaud, P. Vaigot, N. Le Douarin, and E. Dupin, “The instability of the neural crest phenotypes: Schwann cells can differentiate into myofibroblasts,” International Journal of Developmental Biology, vol. 49, no. 2-3, pp. 151–159, 2005.
- T. A. Rizvi, Y. Huang, A. Sidani et al., “A novel cytokine pathway suppresses glial cell melanogenesis after injury to adult nerve,” Journal of Neuroscience, vol. 22, no. 22, pp. 9831–9840, 2002.
- I. Adameyko, F. Lallemend, J. B. Aquino et al., “Schwann cell precursors from nerve innervation are a cellular origin of melanocytes in skin,” Cell, vol. 139, no. 2, pp. 366–379, 2009.
- H. J. S. Stewart, L. Morgan, K. R. Jessen, and R. Mirsky, “Changes in DNA synthesis rate in the Schwann cell lineage in vivo are correlated with the precursor—Schwann cell transition and myelination,” European Journal of Neuroscience, vol. 5, no. 9, pp. 1136–1144, 1993.
- N. Nagoshi, S. Shibata, M. Hamanoue et al., “Schwann cell plasticity after spinal cord injury shown by neural crest lineage tracing,” Glia, vol. 59, no. 5, pp. 771–784, 2011.
- P. Bianco, P. G. Robey, and P. J. Simmons, “Mesenchymal stem cells: revisiting history, concepts, and assays,” Cell Stem Cell, vol. 2, no. 4, pp. 313–319, 2008.
- N. Bertani, P. Malatesta, G. Volpi, P. Sonego, and R. Perris, “Neurogenic potential of human mesenchymal stem cells revisited: analysis by immunostaining, time-lapse video and microarray,” Journal of Cell Science, vol. 118, no. 17, pp. 3925–3936, 2005.
- K. Montzka, N. Lassonczyk, B. Tschöke et al., “Neural differentiation potential of human bone marrow-derived mesenchymal stromal cells: misleading marker gene expression,” BMC Neuroscience, vol. 10, article no. 16, 2009.
- M. Kitada, “Mesenchymal cell populations: development of the induction systems for Schwann cells and neuronal cells and finding the unique stem cell population,” Anatomical Science International, vol. 87, no. 1, pp. 24–44, 2012.
- T. M. Jiang, Z. J. Yang, C. Z. Kong, and H. T. Zhang, “Schwann-like cells can be induction from human nestin-positive amniotic fluid mesenchymal stem cells,” In Vitro Cellular and Developmental Biology, vol. 46, no. 9, pp. 793–800, 2010.
- Y. Wang, W. He, H. Bian, C. Liu, and S. Li, “Small molecule induction of neural-like cells from bone marrow-mesenchymal stem cells,” Journal of Cellular Biochemistry, vol. 113, no. 5, pp. 1527–1536, 2012.
- D. T. Covas, R. A. Panepucci, A. M. Fontes et al., “Multipotent mesenchymal stromal cells obtained from diverse human tissues share functional properties and gene-expression profile with CD146+ perivascular cells and fibroblasts,” Experimental Hematology, vol. 36, no. 5, pp. 642–654, 2008.
- A. Ghodsizad, T. Voelkel, J. M. Moebius, I. Gregoric, V. Bordel, et al., “Biological similarities between mesenchymal stem cells (Mscs) and fibroblasts,” Journal of Cytology & Histology, vol. 1, pp. 1–6, 2010.
- V. Paunescu, F. M. Bojin, C. A. Tatu et al., “Tumour-associated fibroblasts and mesenchymal stem cells: more similarities than differences,” Journal of Cellular and Molecular Medicine, vol. 15, no. 3, pp. 635–646, 2011.
- E. Alt, Y. Yan, S. Gehmert et al., “Fibroblasts share mesenchymal phenotypes with stem cells, but lack their differentiation and colony-forming potential,” Biology of the Cell, vol. 103, no. 4, pp. 197–208, 2011.
- M. Dominici, K. Le Blanc, I. Mueller et al., “Minimal criteria for defining multipotent mesenchymal stromal cells. The international society for cellular therapy position statement,” Cytotherapy, vol. 8, no. 4, pp. 315–317, 2006.
- P. Mafi, S. Hindocha, R. Mafi, and W. Khan, “Adult mesenchymal stem cells and cell surface characterization—a systematic review of the literature,” The Open Orthopaedics Journal, vol. 5, pp. 253–260, 2011.
- Y. Takashima, T. Era, K. Nakao et al., “Neuroepithelial cells supply an initial transient wave of MSC differentiation,” Cell, vol. 129, no. 7, pp. 1377–1388, 2007.