Differential Secondary Reconstitution of In Vivo-Selected Human SCID-Repopulating Cells in NOD/SCID versus NOD/SCID/γ chainnull Mice
Figure 8
Representative example of multilineage engraftment in secondary recipient NSG mice transplanted with BM from vehicle- or 6BG/BCNU-treated mice. The percentage of total human (CD45+) and transduced human progenitors (CD34+), B-lymphoid (CD19+), and myeloid (CD33+) cells in the bone marrow were determined via flow cytometry at 8 weeks after transplant in secondary recipient NSG mice. The percentage in the upper right-hand quadrant represents the percentage of the human cells that are EGFP+ in the progenitor, B-lymphoid, or myeloid cell subsets. The source of the bone-marrow cells that were transplanted into the secondary recipient mice were derived from vehicle- or 6BG/BCNU-treated mice primary NSG mice.
