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Bone Marrow Research
Volume 2011 (2011), Article ID 252953, 11 pages
Differential Secondary Reconstitution of In Vivo-Selected Human SCID-Repopulating Cells in NOD/SCID versus NOD/SCID/γ chainnull Mice
1Section of Pediatric Hematology/Oncology, Department of Pediatrics, Herman B Wells Center for Pediatric Research, Indiana University Simon Cancer Center, The Riley Hospital for Children, 980 West Walnut Street, R3 516, Indianapolis, IN 46202-5525, USA
2Indiana University Simon Cancer Center (IUSCC), Indiana University School of Medicine (IUSM), Indianapolis, IN 46202, USA
3In Vivo Therapeutics Core, IUSCC, Indianapolis, IN 46202, USA
4Biostatistics and Data Management Core, IUSCC, Indianapolis, IN 46202, USA
5Northside Gastroenterology, St. Vincent Hospital, Indianapolis, IN 46260, USA
Received 2 August 2010; Revised 21 October 2010; Accepted 27 October 2010
Academic Editor: A. Ganser
Copyright © 2011 Shanbao Cai et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Humanized bone-marrow xenograft models that can monitor the long-term impact of gene-therapy strategies will help facilitate evaluation of clinical utility. The ability of the murine bone-marrow microenvironment in NOD/SCID versus NOD/SCID/γ chainnull mice to support long-term engraftment of MGMTP140K-transduced human-hematopoietic cells following alkylator-mediated in vivo selection was investigated. Mice were transplanted with MGMTP140K-transduced CD34+ cells and transduced cells selected in vivo. At 4 months after transplantation, levels of human-cell engraftment, and MGMTP140K-transduced cells in the bone marrow of NOD/SCID versus NSG mice varied slightly in vehicle- and drug-treated mice. In secondary transplants, although equal numbers of MGMTP140K-transduced human cells were transplanted, engraftment was significantly higher in NOD/SCID/γ chainnull mice compared to NOD/SCID mice at 2 months after transplantation. These data indicate that reconstitution of NOD/SCID/γ chainnull mice with human-hematopoietic cells represents a more promising model in which to test for genotoxicity and efficacy of strategies that focus on manipulation of long-term repopulating cells of human origin.
Development of humanized hematopoietic xenograft models that can monitor both short- and long-term reconstitution of human hematopoietic stem and progenitor cells (HSCs) will be a necessity as hematopoietic stem cell gene therapy trials continue to move forward and show promise in the clinic [1–7]. Clearly the use of small and large animal models has been an intricate component in paving the way for these successes [8–24]. Adverse events, however, directly related to retroviral-mediated insertional mutagenesis in human trials, have been reported in some patients enrolled in gene-therapy clinical trials for severe combined immunodeficiency (SCID)-X1 [25, 26] and chronic granulomatous disease [7, 27]. In addition, as preclinical studies in nonhuman primates, dogs, and mice have progressed over the past decade, development of leukemias most likely linked or initiated by vector-mediated insertional mutagenesis have been reported [28–33]. Therefore, more investigations are needed to understand retroviral-mediated genome instability in addition to the design of new vector systems to prevent this adverse event in human gene-therapy trials. The evaluation of both short- and long-term reconstituting human HSC will be critical in addressing these issues and is the focus of the current study.
In the past, in vivo experiments designed to study the engraftment and multilineage differentiation of human HSC were challenging since residual immune reactivity in the animals could result in loss of transplanted human HSC. Additionally, transplant of human HSC into NOD/SCID mice, for example, was typically a short-term 3-4 month assay. With the introduction of new immunodeficient strains such as the NOD/SCID/γ chainnull mouse (NSG), long-term analysis of engrafted human hematopoietic cells is now a reality and represents a significant improvement over the commonly used NOD/SCID mouse for studies focused on in vivo analysis of human HSC function . The NSG mice are deficient in the Interleukin-2 receptor (IL2R) common γ chain which is an essential component of multiple cytokine receptors-IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21; this mutation results in a substantial decrease in overall immune function in this mouse strain. In contrast to the NOD/SCID mouse, this additional defect in NSG mice causes a significant block in natural killer-cell development and makes them less susceptible to lymphoma development which allows for an increased life span (NSG = 16 months versus NOD/SCID = 8–10 months). With the increased life span, these mice should be amendable to long-term followup of human HSC function.
Studies using small and large animal models continue to investigate the consequences of genotoxic stress on retrovirally transduced HSC. A powerful tool for in-vivo selection of HSC, a mutant form of O6-methylguanine DNA methyltransferase DNA repair protein (MGMT)-MGMTP140K-has shown promise in nonhuman primates, mice, and humanized xenograft models. Overexpression of MGMTP140K in HSC, which is resistant to the MGMT inhibitor, O6-benzylguanine (6BG) allows for selection and protection of the MGMTP140K-transduced HSC following administration of 6BG in combination with alkylators such as BCNU, CCNU, or temozolomide . If MGMTP140K expression is adequate in the HSC, it should also protect the HSC from high-dose alkylator therapy required in some cancer treatments and thereby prevent therapy-induced myelotoxicity. Generation of HSC that efficiently repair DNA damage due to chemotherapy may protect patients from life-threatening cytopenias commonly observed following dose-intensified therapy. A case in point, in recent phase II clinical trials, patients with nitrosourea-resistant gliomas were simultaneously treated with 6BG to deplete MGMT in the cancer cells, followed by treatment with the DNA-damaging agents, BCNU or temozolomide [36, 37]. Although lack of tumor progression was transiently observed in some patients, effective dose-escalation therapy could not be achieved due to severe hematopoietic toxicity. These studies provide clinical proof that strategies protecting HSC during dose-intensified therapy are indeed clearly needed in relapsed patients requiring high-dose alkylator therapy. In addition, expression of MGMTP140K in HSC can be used as a means to preferentially select and amplify small populations of MGMTP140K-transduced cells in the bone marrow. However, long-term impact of this treatment in humanized mouse models which include secondary transplantation has not been investigated. To what extent administration of even low doses of chemotherapeutic drugs such as BCNU or TMZ could have on long-term human hematopoiesis is not well understood and secondary malignancies are always a concern.
Repetitive low-dose treatment for in vivo selection of MGMTP140K-transduced cells has been successful in mice and large animal models [8, 16]. Numerous transplant studies have convincingly proven that long-term repopulating murine stem cells could be selected in vivo with 6BG/BCNU, 6BG/TMZ, or 6BG/CCNU [9, 11, 14, 17, 18, 20, 21, 38–40]. In regards to modeling of this approach with human HSC, we and others previously demonstrated that MGMTP140K-transduced SCID-repopulating cells and their progeny could be selected in vivo in NOD/SCID mice [19, 24]. Human HSC derived from umbilical cord blood (UCB) or granulocyte colony-stimulating factor (G-CSF)-mobilized peripheral blood (MPB) that expressed MGMTP140K could be selected in vivo by nonmyeloablative doses of 6BG and BCNU. Zielske et al. also reported similar results using MGMTP140K-transduced UCB in the NOD/SCID xenograft model . Additionally, our laboratory went on to investigate the extent to which MGMTP140K-transduced human SCID-repopulating cells and progeny could be protected in vivo by MGMTP140K expression during delivery of high-dose alkylator therapy that kills cancer cells. In this study, we compared the outcome of administering a low-dose 6BG/BCNU regimen versus a high-dose regimen in NOD/SCID mice transplanted with MGMTP140K-transduced mobilized peripheral blood CD34+ cells. We found that, at least in the NOD/SCID xenograft model, when human MPB were transduced with an oncoretroviral vector that expresses MGMTP140K, only low numbers of human MPB cells were protected following delivery of the myeloablative regimen and that these cells were limited to mature lymphoid and myeloid cells . In all these studies, NOD/SCID mice were used and analysis of long-term reconstitution in secondary recipient mice was not determined.
The objective of our current study was to determine to what degree long-term human SCID-repopulating cells could be selected in vivo by alkylator therapy and to compare the levels of selection in primary and secondary NOD/SCID and NSG mice. Our data demonstrate that human hematopoietic cells of multiple lineages were capable of expressing MGMTP140K for at least 4 months in primary recipients and in vivo-selected populations while not as robust as nonselected populations, were able to home and engraft in the bone marrow of secondary recipient NSG months for at least an additional 2 months. In contrast to the NOD/SCID xenograft model, the NSG bone-marrow microenvironment appears to allow for optimal reconstitution and feasibility of long-term followup of human hematopoiesis.
2. Materials and Methods
Breeding colonies of NOD.Cg-Prkdcscid (NOD/SCID) and NOD.Cg-Prkdcscid IL2rgtm1Wjl/Sz (NSG) mice  was established at the Laboratory Animal Research Center and maintained in the In Vivo Therapeutics Core at the Indiana University Simon Cancer Center (IUSCC) (Indianapolis, IN). All protocols and were approved by the Institutional Animal Care and Use Committee at the Indiana University School of Medicine.
2.2. Isolation of Umbilical Cord Blood (UCB) CD34+ Cells
All protocols were approved by Indiana University School of Medicine’s Institutional Review Board (IRB) and St. Vincent Hospital’s IRB (Indianapolis, IN). Samples of UCB were collected from normal, full-term infants delivered by cesarean section and the CD34+ cells isolated using the CD34 MicroBead kit and VarioMACS Separator (Miltenyi Biotech Inc., Auburn, CA) according to the manufacturer’s instructions.
2.3. Retrovirus Backbones for Expression of MGMTP140K in CD34+ Cells
The oncoretroviral vector, SF1-MGMTP140K-IRES-EGFP (SF1-P140K) was utilized to coexpress MGMTP140K and EGFP in human CD34+ cells and has been described previously . Retroviral vectors were pseudotyped with the gibbon ape leukemia virus envelope (GALV) using the PG13 packaging cell line (American Type Culture Collection, Manassas, Va.) . Titers were initially determined on human erythroleukemia (HEL) cells by limiting-dilution analysis.
2.4. Transduction of UCB CD34+ Cells
The transductions were done as previously described by our laboratory [10, 19, 42]. The starting cell number prior to prestimulation and transduction was 4 × 105 per mouse transplanted. Isolated CD34+ cells were prestimulated at a cell density of 5 × 105 cells per ml in Ex Vivo-10 serum-free medium-containing 1% human serum albumin. The medium was supplemented with Granulocyte-Colony Stimulating Factor (G-CSF), stem cell factor (SCF), and thrombopoietin (TPO) (PeproTech, Rocky Hill, NJ). Each cytokine was used at 100 ng/ml for prestimulation. Nontissue culture 10-cm plates (Falcon, Franklin Lakes, NJ), were coated with 2 μg/cm2 Retronectin—(Takara Shuzo, Otsu, Japan) overnight at 4°C. Cells were plated at a concentration of 2 × 105 cells per cm2 for transduction. Cells were infected with a 1 : 1 ratio of retrovirus supernatant : complete media with cytokines for 4 hours on 2 consecutive days, with a change to complete medium-containing cytokines for overnight incubation. After the second round of infection, cells were allowed to remain overnight on Retronectin-coated plates with fresh medium and cytokines and then transplanted. The transduction efficiency was determined by flow cytometry on the day of the transplant. For secondary transplants, bone-marrow cells were resuspended at 1 × 106 per ml in Ex Vivo-10 serum-free medium-containing 1% human serum albumin, 100 ng/ml SCF, and 100 ng/ml IL-6 (PeproTech) for 36–48 hours prior to transplantation.
2.5. Transplantation of NOD/SCID and NSG Mice with Human CD34+ Cells
Immunodeficient mice were placed on food pellets containing 0.0625% doxycycline for 5–7 days prior to irradiation. Mice were then conditioned with 300-cGy total-body irradiation using a GammaCell 40 (Nordion International Inc., Ontario Canada) equipped with two opposing Cesium-137 sources. UCB CD34+ cells were resuspended in IMDM containing 0.2% endotoxin-free bovine serum albumin and injected into the lateral tail vein of each animal.
2.6. Chemotherapy Administration
O6-benzylguanine (6BG) (Sigma-Aldrich, St Louis, MO) was dissolved in 40% polyethylene glycol-400 (v/v) and 60% saline (v/v). BCNU (Sigma) was dissolved in 10% ethanol (v/v) and 90% normal saline solution (v/v). BCNU was placed on ice and used immediately after reconstitution. One cycle of treatment consisted of 20 mg/kg 6BG followed by 5 mg/kg BCNU one-hour later. Two cycles of treatment were delivered one week apart.
2.7. Analysis of Human Cell Engraftment
Mice were sacrificed at 8 weeks after 6BG/BCNU injection and single-cell suspensions of the bone marrow (BM) prepared as previously described [10, 19, 42]. Human cell engraftment measured by human CD45 staining and the proportion of engraftment in various lineages was determined by immunostaining and flow cytometric analysis. Aliquots of 1-2 × 105 cells/tube were stained with various antibodies for 25 minutes at 4°C in complete medium and washed 1 time in PBS containing 1% FBS. All antibodies were titered and used at saturating concentrations. The lack of crossreactivity of human-specific antibodies with murine cells was confirmed in every experiment by staining BM from a nontransplanted mouse with each antibody combination. Cells were stained with Allophycocyanin (APC)-conjugated anti-human-CD45 (anti-HLe-1; Becton Dickinson Immunocytometry, San Jose, CA) alone or in combination with phycoerthyrin (PE)-conjugated anti-human CD33 (anti-Leu-M9; Becton Dickinson). Identical aliquots were stained with APC-conjugated anti-human CD34 (clone 581; PharMingen, San Diego, CA) in combination with anti-human CD19-PE (PharMingen) or anti-human CD38-PE (anti-Leu-17; Becton Dickinson). The forward and right-angle light scatter parameters were used to set the gates for analysis. In experiments where engraftment of human cells was >5%, ~20,000–40,000 events were collected and analyzed. In experiments in which human engraftment was <5% ~200,000 events were collected and analyzed. All samples were acquired and analyzed on a Becton-Dickinson FACSCalibur using CellQuest software (Becton-Dickinson).
2.8. Statistical Analysis
The Mann-Whitney -test was utilized to determine statistical significance. Differences between mouse cohorts were considered significant at using two-sided tests.
3.1. Comparison of Human Hematopoietic Cell Engraftment in NSG versus NOD/SCID Mice
In order to gain a better understanding of the engraftment capabilities of NSG and NOD/SCID mice, we first evaluated the engraftment of nontransduced UCB CD34+ cells transplanted into NSG versus NOD/SCID mice. Cohorts of sublethally irradiated NSG and NOD/SCID mice were transplanted with increasing numbers of nonmanipulated, freshly isolated UCB CD34+ cells (1.0–5.0 × 104 per mouse). At 2 months after transplantation, the bone marrow was harvested and analyzed for the engraftment of human cells (Figure 1). Human hematopoietic cells engrafted in all NSG and NOD/SCID mice but at higher levels in NSG versus NOD/SCID mice. Engraftment levels in the NSG mice was ~3-fold higher than levels observed in the NOD/SCID mice.
3.2. 6BG/BCNU-Mediated In Vivo Selection of MGMTP140K-Transduced UCB CD34+ Cells in Primary Recipient NOD/SCID and NSG Mice
Human umbilical cord blood CD34+ cells were next transduced with a GALV-pseudotyped oncoretroviral vector, SF1-MGMTP140K-IRES-EGFP previously shown by our group to express high levels of MGMTP140K in NOD/SCID mice transplanted with MGMTP140K-transduced human cells . The transduction efficiency was determined by EGFP expression in bulk CD34+ cells and clonogenic cells and was 65% and 60% EGFP+, respectively, (data not shown). Equal numbers of transduced cells were transplanted into sublethally irradiated (300 cGy) NSG or NOD/SCID mice (Figure 2). A relatively large number of UCB CD34+ cells were transplanted per mouse (4 × 105 total CD34+ cells) so sufficient numbers of human cells could be obtained for the secondary transplants following the in vivo selection and recovery period. One month after transplantation, mice received 2 cycles administered one week apart consisting of 20 mg/kg 6BG followed one hour later with 5 mg/kg BCNU. At 4 months after transplantation, the bone marrow was harvested and analyzed for the presence of human cells by flow cytometry. Both mouse strains contained high levels of human-cell engraftment in the bone marrow (Figure 3(a)) and in vivo selection of transduced cells with 6BG/BCNU was achieved in both mouse strains (Figure 3(b)). However, the overall human engraftment (human CD45+ cells) in vehicle-treated NOD/SCID mice was slightly decreased compared to engraftment in vehicle-treated NSG mice (Figure 3(a), vehicle-treated NOD/SCID mice-69% ± 8% versus vehicle-treated NSG mice-81% ± 4). In addition, the variability of human-cell engraftment within the drug-treated NOD/SCID cohort was more pronounced than that seen in the drug-treated NSG cohort (Figure 3(a), drug-treated NOD/SCID mice-63% ± 32% human CD45+ cells versus drug-treated NSG mice-76% ± 10% human CD45+ cells). In the drug-treated NOD/SCID mice for example, 2/8 exhibited a significant decrease in human chimerism following 6BG/BCNU whereas human-cell engraftment remained more consistent within the NSG drug-treated cohort (Figure 3(a)).
While significant in vivo selection of human MGMTP140K-transduced cells was clearly evident at 4 months after transplant regardless of the mouse strain, these data indicate that the microenvironment of the NSG mouse which is devoid of murine NK-cell activity may be more amendable for the in vivo selection and amplification of transduced human cells. A detailed flow cytometric analysis was also performed to see if any specific cell lineage was preferentially affected by the in vivo selection in the mouse strains. While the variability in the percentages of human CD34+ cells was larger in the drug-treated NOD/SCID mice than the NSG mice, it did not reach statistical significance (Figure 4(a)). Consistent with the overall in vivo selection data presented in Figure 3, there was a significant increase in the in vivo-selected CD34+ cells in vehicle- versus drug-treated regardless of the mouse strain used indicating a selection and some expansion of the human EGFP+, CD34+ cells in the bone marrow (Figure 4(b)). The outcome of 6BG/BCNU treatment on multilineage differentiation typically found in the bone marrow of NOD/SCID and NSG mice reconstituted with human cells was next evaluated (Figure 5). In vehicle-treated primary recipient NSG and NOD/SCID mice, similar percentages of total and transduced CD34+ progenitor, human B-lymphoid, and myeloid cells were present in the human bone-marrow grafts. Human bone-marrow grafts in vehicle-treated NSG and NOD/SCID mice consisted of 10–15% CD34+ progenitor, 65–80% B-lymphoid, and 10–20% myeloid cells, and 20–30% of these lineages expressed EGFP (data not shown). In 6BG/BCNU-treated NSG and NOD/SCID mice, a significant selection of human EGFP+ cells was evident in CD34+ progenitor, CD19+ B-lymphoid and human CD33+ myeloid cells with at least 88% of these cell lineages expressing EGFP+ at 8 weeks after drug treatment (Figure 5).
3.3. Secondary Reconstitution of NOD/SCID and NSG Mice with MGMTP140K-Transduced UCB CD34+ Cells Derived from NOD/SCID and NSG Mice
Peled et al. originally demonstrated that secondary reconstitution of immunodeficient mice is improved by exposure to IL-6 and SCF prior to retransplantation . The primary mechanism for the enhancement in repopulating ability is increased expression of the CXCR-4 receptor previously shown to play a key role in the homing of transplanted cells. In agreement with these observations, we found that IL-6/SCF prestimulation of human bone-marrow cultures derived from the transplanted NOD/SCID or NSG mice led to an increase or at least maintenance of CXCR-4 expression on the surface of the majority of the human cells (data not shown). Following the 48-hour prestimulation period, 10 × 106 human CD45+ cells (vehicle or drug-treated) which contained similar percentages of CD34+ cells were transplanted into sublethally irradiated NOD/SCID or NSG mice as outlined in Figure 2. At 2 months after transplantation, the bone marrow of the secondary recipient mice was analyzed for the percentage of total human CD45+ cells (Figure 6(a)) and EGFP+ human CD45+ cells (Figure 6(b)). Even though equivalent numbers of transduced human cells from primary recipient NOD/SCID and NSG mice were transplanted into secondary recipient mice, striking and statistically significant differences in the level of engraftment between the NOD/SCID and the NSG mice was observed (Figure 6(a)). Low levels of engraftment were observed in secondary NOD/SCID mice receiving human cells from vehicle-treated primary NOD/SCID mice and ranged from 2%–4% human CD45+ cells with 25–45% of these human hematopoietic cells remaining EGFP+. In addition, in NOD/SCID secondary recipient mice transplanted with bone marrow from primary recipient NOD/SCID mice previously drug treated, only 1 of 4 mice showed detectable levels of human cells (1.5% human CD45+ cells). In comparison to the NOD/SCID transplants, different levels of human-cell engraftment were found in the secondary transplant experiments using the NSG mice. Human-cell engraftment was detected in all secondary recipient NSG mice (Figure 6), although engraftment levels were different from that previously observed in the primary transplanted NSG mice. Engraftment of NSG mice receiving humanized bone marrow from vehicle-treated mice was 55%–70% human CD45+ with 15–30% of the cells transduced. Engraftment of secondary NSG mice receiving humanized bone marrow from drug-treated mice ranged from 4%–18% human CD45+ with EGFP+ cells ranging from 15%–95%. While a significant increase in the percentage of EGFP+CD34+ cells was observed in the drug-treated versus the vehicle-treated secondary recipient mice, significant differences were noted in the overall engraftment levels of the CD34+ cells in the secondary recipient NSG mice (Figure 7). A significant decrease in the percentage of CD34+ cells in the grafts derived from the drug-treated mice compared to grafts derived from vehicle-treated mice was evident and is consistent with decreased long-term SCID-repopulating ability in the secondary recipient NSG mice that received bone marrow from drug-treated primary NSG recipients. The impact of 6BG/BCNU treatment on multilineage differentiation of human cells was next evaluated (Figure 8) and a significant selection of EGFP+ human cells was evident in multiple cell lineages in the NSG mice. Increased percentages of EGFP+ cells were found in populations of human hematopoietic CD34+ progenitor, CD19+ B-lymphoid, and CD33+ myeloid cells derived from the bone marrow of drug-treated NSG mice indicating that secondary reconstituting SCID-repopulating cells were capable of multilineage differentiation.
4. Discussion and Conclusions
Maintenance of genome stability in hematopoietic stem and progenitor cells (HSC) following ex vivo manipulation and transplantation will be essential for normal blood-cell development. The objective of this study was to develop a humanized bone-marrow xenograft model to follow long-term reconstitution of genetically modified human HSC. We determined if populations of in vivo-selected MGMTP140K-transduced human HSC residing in the bone marrow still contained sufficient SCID-repopulating cell activity to reconstitute secondary recipient animals. In this initial study, our focus was to define the basic parameters of the model so that adequate engraftment of HSC in secondary recipients could be achieved. The MGMTP140K-transduced human HSC underwent in vivo selection in the primary recipient mice one-month after transplant and then the mice were allowed to recover out to 4-months after transplant. We found that while both the NOD/SCID and NSG models adequately supported human HSC reconstitution and in vivo-selection in the primary recipient mice, NSG mice were far superior to the NOD/SCID model for secondary reconstitution. It will now be possible to extend the post-transplant time frame, and studies are currently in progress in which genetically modified human HSC are being followed for at least a year after transplantation.
With basic parameters of the model defined, we are continuing to develop this valuable xenograft model. Studies are now in progress to test lenti- and foamy-viral vector systems to determine if increased numbers of long-term SCID-repopulating cells can be transduced. In addition, the consistency of long-term transgene expression and also analysis of retroviral-insertion sites in primary and secondary transplanted animals in the absence and presence of in vivo selection will be evaluated in these long-term studies.
Since Natural-Killer cell precursor cells require expression of the common γ chain protein for development into mature NK cells in vivo, lack of this protein will preclude their development. The rejection of human hematopoietic cells by murine NK cells in NOD/SCID mice is most likely the primary reason for decreased engraftment of the human CD34+ cells in the NOD/SCID mice but not in the NSG mice. Others have shown previously that the injection of anti-CD122 inhibits NK activity in NOD/SCID mice and improves engraftment in the bone marrow [34, 44–48]. In order to begin to understand the mechanistic differences seen in the engraftment levels of the two mouse strains, we initially looked at the “engraftability” of nonmanipulated CD34+ cells in primary recipient NSG versus NOD/SCID mice. Differences in the engraftment of the SCID-repopulating cells in primary NSG versus NOD/SCID recipients mice were apparent when low numbers of human CD34+ cells were transplanted. Similar engraftgment results in primary recipient NSG versus NOD/SCID mice have been recently reported by McDermott et al. . It is important to note that our data also demonstrated that when larger numbers of transduced CD34+ cells were initially transplanted into primary recipients NOD/SCID or NOD/SCID/γ chainnull mice, differences in overall human engraftment were evident but were not statistically significant. However, once the chimeric bone-marrow cells were transplanted into secondary recipient animals, engraftment differences in SCID-repopulating activity between NSG and NOD/SCID were statistically significant.
Bone-marrow cellularity was similar in the NSG and NOD/SCID mouse cohorts indicating that the absolute numbers of EGFP+ cells in the bone marrow in the vehicle- and treated-mice in the primary NOD/SCID and NSG mice were also similar. Flow cytometric analyses indicated that the mean fluorescence intensities for EGFP were also similar in the EGFP+ populations found in both strains of mice (data not shown). We have previously shown that EGFP expression does correlate with MGMTP140K expression . Therefore, potential differences in transgene expression most likely do not account for the engraftment differences seen between the NOD/SCID and NSG mice.
While the major cellular difference noted in the original paper by Shultz et al. was the lack of murine NK-cell development , we cannot rule out that the function of other cell types in the bone-marrow microenvironment could be modulated by lack of the common γ chain expression and that this could affect the homing, survival, expansion, and/or differentiation of the human hematopoietic cells. Differential engraftment differences observed in our study are consistent with the hypothesis that the lack of mature murine NK cells in the NSG mice allows for more optimal engraftment, survival, and differentiation of hematopoietic cell subsets since murine NK cells are not present to destroy the human grafts in the NSG mice.
Our data indicate that while we were able to detect human HSC engraftment in secondary NSG mice transplanted with bone marrow from drug-treated primary mice (4%–18% human CD45+), it did not approach the levels of reconstitution seen in secondary NSG mice transplanted with bone marrow from vehicle-treated mice (55%–70% human CD45+). Furthermore, the percentage of EGFP+ cells in secondary NSG mice transplanted with bone marrow from drug-treated primary mice cells varied from 15% to 95%. Transgene expression was more uniform in the drug-treated primary recipient mice (Figure 2(b)). It is not clear at this time whether this decrease in the percent of transduced cells has to do with silencing of the vector and/or decreased SCID-repopulating cell activity in the transduced population. No 6BG/BCNU treatment was administered in the secondary recipient mice and future experiments will also address the outcome of administering in vivo selective pressure in the secondary recipient mice. Transplantation of ex vivo manipulated HSC into NOD/SCID/γ chainnull mice represents a feasible model in which to test and validate novel strategies that focus on therapeutic manipulation of long-term repopulating cells from human stem cell sources. Umbilical-cord blood cells were utilized as the transplant source in this study. It will be important to define the long-term SCID-repopulating cell frequency in human bone marrow and G-CSF-mobilized peripheral blood in the NSG mice since these sources will be used in human gene-therapy trials, and typically have lower primary SCID-repopulating cell frequencies than UCB , and can be more refractive to transduction than UCB-derived HSC . These data indicate that long-lived SCID-repopulating cells can be transduced with oncoretroviral vectors and selected in vivo and offers a promising and unique model in which to assess potential genotoxicity and ultimately optimize in vivo selection strategies.
Hope Street Kids Foundation (KEP); the Riley Children’s Foundation (SC, HW, and KEP); RO1 CA138798 (KEP).
The authors wish to thank Drs. Scott Goebel and Helmut Hanenberg for their support and helpful discussions. They also thank Mary Murray for her support and critical reading of this paper. They thank the nursing staff at the St. Vincent Hospital for donation of their time for collection of umbilical cord blood products. They also acknowledge the continuing support of the Riley Childrens’ Foundation and the In Vivo Therapeutics Core at the Indiana University Simon Cancer Center.
- A. Aiuti, S. Slavin, M. Aker et al., “Correction of ADA-SCID by stem cell gene therapy combined with nonmyeloablative conditioning,” Science, vol. 296, no. 5577, pp. 2410–2413, 2002.
- A. Aiuti, S. Vai, A. Mortellaro et al., “Immune reconstitution in ADA-SCID after PBL gene therapy and discontinuation of enzyme replacement,” Nature Medicine, vol. 8, no. 5, pp. 423–425, 2002.
- M. Cavazzana-Calvo, S. Hacein-Bey, G. de Saint-Basile, F. Le Deist, and A. Fischer, “Gene therapy of severe combined immunodeficiencies,” Transfusion Clinique et Biologique, vol. 7, pp. 259–260, 2000.
- M. Cavazzana-Calvo, S. Hacein-Bey, G. De Saint Basile et al., “Gene therapy of human severe combined immunodeficiency (SCID)-X1 disease,” Science, vol. 288, no. 5466, pp. 669–672, 2000.
- J. Chinen, J. Davis, S. S. De Ravin et al., “Gene therapy improves immune function in preadolescents with X-linked severe combined immunodeficiency,” Blood, vol. 110, no. 1, pp. 67–73, 2007.
- D. B. Kohn and F. Candotti, “Gene therapy fulfilling its promise,” The New England Journal of Medicine, vol. 360, no. 5, pp. 518–521, 2009.
- M. G. Ott, M. Schmidt, K. Schwarzwaelder et al., “Correction of X-linked chronic granulomatous disease by gene therapy, augmented by insertional activation of MDS1-EVI1, PRDM16 or SETBP1,” Nature Medicine, vol. 12, no. 4, pp. 401–409, 2006.
- B. C. Beard, G. D. Trobridge, C. Ironside, J. S. McCune, J. E. Adair, and H.-P. Kiem, “Efficient and stable MGMT-mediated selection of long-term repopulating stem cells in nonhuman primates,” Journal of Clinical Investigation, vol. 120, no. 7, pp. 2345–2354, 2010.
- S. Cai, A. Ernstberger, H. Wang et al., “In vivo selection of hematopoietic stem cells transduced at a low multiplicity-of-infection with a foamy viral MGMTP140K vector,” Experimental Hematology, vol. 36, no. 3, pp. 283–292, 2008.
- S. Cai, J. R. Hartwell, R. J. Cooper et al., “In vivo effects of myeloablative alkylator therapy on survival and differentiation of MGMTP140K-transduced human G-CSF-mobilized peripheral blood cells,” Molecular Therapy, vol. 13, no. 5, pp. 1016–1026, 2006.
- B. M. Davis, L. Humeau, and B. Dropulic, “In vivo selection for human and murine hematopoietic cells transduced with a therapeutic MGMT lentiviral vector that inhibits HIV replication,” Molecular Therapy, vol. 9, no. 2, pp. 160–172, 2004.
- C. E. Dunbar and A. Larochelle, “Gene therapy activates EVI1, destabilizes chromosomes,” Nature Medicine, vol. 16, no. 2, pp. 163–165, 2010.
- Y.-J. Kim, Y.-S. Kim, A. Larochelle et al., “Sustained high-level polyclonal hematopoietic marking and transgene expression 4 years after autologous transplantation of rhesus macaques with SIV lentiviral vector-transduced CD34+ cells,” Blood, vol. 113, no. 22, pp. 5434–5443, 2009.
- E. L. Kreklau, K. E. Pollok, B. J. Bailey et al., “Hematopoietic expression of -methylguanine DNA methyltransferase-P140K allows intensive treatment of human glioma xenografts with combination -benzylguanine and 1,3-bis-(2-chloroethyl)-1-nitrosourea,” Molecular Cancer Therapeutics, vol. 2, no. 12, pp. 1321–1329, 2003.
- A. Larochelle, U. Choi, Y. Shou et al., “In vivo selection of hematopoietic progenitor cells and temozolomide dose intensification in rhesus macaques through lentiviral transduction with a drug resistance gene,” Journal of Clinical Investigation, vol. 119, no. 7, pp. 1952–1963, 2009.
- T. Neff, B. C. Beard, L. J. Peterson, P. Anandakumar, J. Thompson, and H.-P. Kiem, “Polyclonal chemoprotection against temozolomide in a large-animal model of drug resistance gene therapy,” Blood, vol. 105, no. 3, pp. 997–1002, 2005.
- D. A. Persons, E. R. Allay, N. Sawai et al., “Successful treatment of murine β-thalassemia using in vivo selection of genetically modified, drug-resistant hematopoietic stem cells,” Blood, vol. 102, no. 2, pp. 506–513, 2003.
- D. A. Persons, J. A. Allay, A. Bonifacino et al., “Transient in vivo selection of transduced peripheral blood cells using antifolate drug selection in rhesus macaques that received transplants with hematopoietic stem cells expressing dihydrofolate reductase vectors,” Blood, vol. 103, no. 3, pp. 796–803, 2004.
- K. E. Pollok, J. R. Hartwell, A. Braber et al., “In vivo selection of human hematopoietic cells in a xenograft model using combined pharmacologic and genetic manipulations,” Human Gene Therapy, vol. 14, no. 18, pp. 1703–1714, 2003.
- S. Ragg, M. Xu-Welliver, J. Bailey et al., “Direct reversal of DNA damage mutant methyltransferase protein protects mice against dose-intensified chemotherapy and leads to in vivo selection of hematopoietic stem cells,” Cancer Research, vol. 60, no. 18, pp. 5187–5195, 2000.
- N. Sawai, D. A. Persons, S. Zhou, T. Lu, and B. P. Sorrentino, “Reduction in hematopoietic stem cell numbers with in vivo drug selection can be partially abrogated by HOXB4 gene expression,” Molecular Therapy, vol. 8, no. 3, pp. 376–384, 2003.
- S. Sellers, T. J. Gomes, A. Larochelle et al., “Ex vivo expansion of retrovirally transduced primate CD34+ cells results in overrepresentation of clones with MDS1/evi1 insertion sites in the myeloid lineage after transplantation,” Molecular Therapy, vol. 18, no. 9, pp. 1633–1639, 2010.
- J. Xie, A. Larochelle, I. Maric, M. Faulhaber, R. E. Donahue, and C. E. Dunbar, “Repetitive busulfan administration after hematopoietic stem cell gene therapy associated with a dominant HDAC7 clone in a nonhuman primate,” Human Gene Therapy, vol. 21, no. 6, pp. 695–703, 2010.
- S. P. Zielske, J. S. Reese, K. T. Lingas, J. R. Donze, and S. L. Gerson, “In vivo selection of MGMT(P140K) lentivirus-transduced human NOD/SCID repopulating cells without pretransplant irradiation conditioning,” Journal of Clinical Investigation, vol. 112, no. 10, pp. 1561–1570, 2003.
- S. Hacein-Bey-Abina, C. Von Kalle, M. Schmidt et al., “A serious adverse event after successful gene therapy for X-linked severe combined immunodeficiency,” The New England Journal of Medicine, vol. 348, no. 3, pp. 255–256, 2003.
- S. Hacein-Bey-Abina, C. Von Kalle, M. Schmidt et al., “LMO2 -associated clonal T cell proliferation in two patients after gene therapy for SCID-X1,” Science, vol. 302, no. 5644, pp. 415–419, 2003.
- J.-Y. Métais and C. E. Dunbar, “The MDS1-EVI1 gene complex as a retrovirus integration site: impact on behavior of hematopoietic cells and implications for gene therapy,” Molecular Therapy, vol. 16, no. 3, pp. 439–449, 2008.
- X. Li, M. M. Le Beau, S. Ciccone et al., “Ex vivo culture of stem/progenitor cells predisposes cells to undergo apoptosis, and surviving stem/progenitor cells display cytogenetic abnormalities and an increased risk of malignancy,” Blood, vol. 105, no. 9, pp. 3465–3471, 2005.
- Z. Li, J. Düllmann, B. Schiedlmeier et al., “Murine leukemia induced by retroviral gene marking,” Science, vol. 296, no. 5567, p. 497, 2002.
- U. Modlich, O. S. Kustikova, M. Schmidt et al., “Leukemias following retroviral transfer of multidrug resistance 1 (MDR1) are driven by combinatorial insertional mutagenesis,” Blood, vol. 105, no. 11, pp. 4235–4246, 2005.
- A. W. Nienhuis, C. E. Dunbar, and B. P. Sorrentino, “Genotoxicity of retroviral integration in hematopoietic cells,” Molecular Therapy, vol. 13, no. 6, pp. 1031–1049, 2006.
- R. Seggewiss, S. Pittaluga, R. L. Adler et al., “Acute myeloid leukemia is associated with retroviral gene transfer to hematopoietic progenitor cells in a rhesus macaque,” Blood, vol. 107, no. 10, pp. 3865–3867, 2006.
- X.-B. Zhang, B. C. Beard, G. D. Trobridge et al., “High incidence of leukemia in large animals after stem cell gene therapy with a HOXB4-expressing retroviral vector,” Journal of Clinical Investigation, vol. 118, no. 4, pp. 1502–1510, 2008.
- L. D. Shultz, B. L. Lyons, L. M. Burzenski et al., “Human lymphoid and myeloid cell development in NOD/LtSz-scid IL2Rγnull mice engrafted with mobilized human hemopoietic stem cells,” Journal of Immunology, vol. 174, no. 10, pp. 6477–6489, 2005.
- S. L. Gerson, “Clinical relevance of MGMT in the treatment of cancer,” Journal of Clinical Oncology, vol. 20, no. 9, pp. 2388–2399, 2002.
- J. A. Quinn, S. X. Jiang, D. A. Reardon et al., “Phase II trial of temozolomide plus -benzylguanine in adults with recurrent, temozolomide-resistant malignant glioma,” Journal of Clinical Oncology, vol. 27, no. 8, pp. 1262–1267, 2009.
- J. A. Quinn, J. Pluda, M. E. Dolan et al., “Phase II trial of carmustine plus -benzylguanine for patients with nitrosourea-resistant recurrent or progressive malignant glioma,” Journal of Clinical Oncology, vol. 20, no. 9, pp. 2277–2283, 2002.
- M. Jansen, U. R. Sorg, S. Ragg et al., “Hematoprotection and enrichment of transduced cells in vivo after gene transfer of MGMTP140k into hematopoietic stem cells,” Cancer Gene Therapy, vol. 9, no. 9, pp. 737–746, 2002.
- M. D. Milsom, L. B. Woolford, G. P. Margison, R. K. Humphries, and L. J. Fairbairn, “Enhanced in vivo selection of bone marrow cells by retroviral-mediated coexpression of mutant -methylguanine-DNA-methyltransferase and HOXB4,” Molecular Therapy, vol. 10, no. 5, pp. 862–873, 2004.
- N. Sawai, S. Zhou, E. F. Vanin, P. Houghton, T. P. Brent, and B. P. Sorrentino, “Protection and in vivo selection of hematopoietic stem cells using temozolomide, -benzylguanine, and an alkyltransferase-expressing retroviral vector,” Molecular Therapy, vol. 3, no. 1, pp. 78–87, 2001.
- A. D. Miller, J. V. Garcia, N. von Suhr, C. M. Lynch, C. Wilson, and M. V. Eiden, “Construction and properties of retrovirus packaging cells based on gibbon ape leukemia virus,” Journal of Virology, vol. 65, no. 5, pp. 2220–2224, 1991.
- K. E. Pollok, J. C. M. Van der Loo, R. J. Cooper et al., “Differential transduction efficiency of SCID-repopulating cells derived from umbilical cord blood and granulocyte colony-stimulating factor-mobilized peripheral blood,” Human Gene Therapy, vol. 12, no. 17, pp. 2095–2108, 2001.
- A. Peled, I. Petit, O. Kollet et al., “Dependence of human stem cell engraftment and repopulation of NOD/SCID mice on CXCR4,” Science, vol. 283, no. 5403, pp. 845–848, 1999.
- S. P. McDermott, K. Eppert, E. R. Lechman, M. Doedens, and J. E. Dick, “Comparison of human cord blood engraftment between immunocompromised mouse strains,” Blood, vol. 116, no. 2, pp. 193–200, 2010.
- J. L. McKenzie, O. I. Gan, M. Doedens, and J. E. Dick, “Human short-term repopulating stem cells are efficiently detected following intrafemoral transplantation into NOD/SCID recipients depleted of CD122+ cells,” Blood, vol. 106, no. 4, pp. 1259–1261, 2005.
- L. D. Shultz, S. J. Banuelos, J. Leif et al., “Regulation of human short-term repopulating cell (STRC) engraftment in NOD/SCID mice by host CD122+ cells,” Experimental Hematology, vol. 31, no. 6, pp. 551–558, 2003.
- L. D. Shultz, P. A. Schweitzer, S. W. Christianson et al., “Multiple defects in innate and adaptive immunologic function in NOD/LtSz- scid mice,” Journal of Immunology, vol. 154, no. 1, pp. 180–191, 1995.
- T. Tanaka, F. Kitamura, Y. Nagasaka, K. Kuida, H. Suwa, and M. Miyasaka, “Selective long-term elimination of natural killer cells in vivo by an anti-interleukin 2 receptor β chain monoclonal antibody in mice,” Journal of Experimental Medicine, vol. 178, no. 3, pp. 1103–1107, 1993.
- A. Larochelle, J. Vormoor, H. Hanenberg et al., “Identification of primitive human hematopoietic cells capable of repopulating NOD/SCID mouse bone marrow: implications for gene therapy,” Nature Medicine, vol. 2, no. 12, pp. 1329–1337, 1996.