Research Article

Virus-Specific Read-Through Codon Preference Affects Infectivity of Chimeric Cucumber Green Mottle Mosaic Viruses Displaying a Dengue Virus Epitope

Table 3

Summary of sequence analyses carried out to confirm the presence of EB4 within the putative recombinant CGMMV. (#A truncated EB4 sequence was detected at 30 dpi according to Figure 7 for pR_P3U4 clone from nucleotide position 702 to 746.)

Presence of EB4 within putative chimeric CGMMV genome

Sample RNA source used for RT-PCR
Transcript-inoculated Virus particles
plant total RNAtotal RNA
14 dpi 21 dpi 30 dpi

Chimeric clone PresentPresent in #Not present
(with pCG+FSRTRE <50% of
transcripts)plants tested