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Journal of Biomedicine and Biotechnology
Volume 2011 (2011), Article ID 148763, 9 pages
http://dx.doi.org/10.1155/2011/148763
Research Article

Hydrodynamic Delivery of Chitosan-Folate-DNA Nanoparticles in Rats with Adjuvant-Induced Arthritis

1Orthopaedics Research Laboratory, Research Centre, Sacré-Coeur Hospital, University of Montreal, 5400 West Gouin Boulevard, Montreal, QC, Canada H4J 1C5
2Faculty of Pharmacy and Department of Chemistry, University of Montreal, CP 6128 Succursale Centre Ville, Montreal, QC, Canada H3C 3J7
3The key laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Jiao Tong University School of Medicine (SJTUSM), Shanghai 200025, China
4Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences (CAS), 225 South Chongqing Road, Shanghai 200025, China

Received 14 September 2010; Accepted 29 November 2010

Academic Editor: Oreste Gualillo

Copyright © 2011 Qin Shi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

50 kDa chitosan was conjugated with folate, a specific tissue-targeting ligand. Nanoparticles such as chitosan-DNA and folate-chitosan-DNA were prepared by coacervation process. The hydrodynamic intravenous injection of nanoparticles was performed in the right posterior paw in normal and arthritic rats. Our results demonstrated that the fluorescence intensity of DsRed detected was 5 to 12 times more in the right soleus muscle and in the right gastro muscle than other tissue sections. β-galactosidase gene expression with X-gal substrate and folate-chitosan-plasmid nanoparticles showed best coloration in the soleus muscle. Treated arthritic animals also showed a significant decrease in paw swelling and IL-1β and PGE2 concentration in serum compared to untreated rats. This study demonstrated that a nonviral gene therapeutic approach using hydrodynamic delivery could help transfect more efficiently folate-chitosan-DNA nanoparticles in vitro/in vivo and could decrease inflammation in arthritic rats.