A New Microarray System to Detect Streptococcus pneumoniae Serotypes

Table 4

Microarray results of each strain.

	Serotype	Strain ID	Positive probe^a	Microarray result
					Assined group
23 serotypes included in 23-valent vaccine	1	ATCC6301	1, 2, 3, 4, 5, 6	Perfectly matched	1
	2	ATCC6302	7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18	Perfectly matched	2
	3	D36	19, 20, 21	Perfectly matched	3
	4	JHK27	22, 23, 24, 25, 26, 27, 28, 29, 30	Perfectly matched	4
	5	ATCC6305	23, 24, 31, 32, 33, 34, 35, 36, 37	Perfectly matched	5
	6B	MSC1047	38, 39, 41, 42, 43	1 probe of group 6A/6B did not hybridized	6A/6B
	7F	ATCC10351	44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58	Perfectly matched	7F/7A
	8	ATCC6308	59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70	Perfectly matched	8
	9V	KD10-11	71, 72, 73, 74, 75, 76, 77, 78, 84, 85, 81, 82, 83	Perfectly matched	9A/9V
	9N	KD01-26	71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83	Perfectly matched	9L/9N
	10A	ATCC8334	86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100	Perfectly matched	10A
	11A	SSI11A/2	101, 102, 103, 104, 105, 106, 107, 108, 109	Perfectly matched	11A/11D
	12F	ATCC6312	11, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124	1 extra probe of group 2 hybridized	12F/12A/12B/44/46
	14	D59	101, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137	Perfectly matched	14
	15B	ATCC10354	125, 128, 131, 135, 138, 139, 140, 141, 142, 143	Perfectly matched	15B/15C
	17F	ATCC6317	144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156	1 extra probe of group 18B/18C hybridized	17F
	18C	ATCC10356	156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166	Perfectly matched	18B/18C
	19F	D33	72, 167, 168,169, 171, 172, 173	Perfectly matched	19F
	19A	D4	71, 73, 74, 169, 170, 171, 172, 173,	1 extra probe of group 19F hybridized	19A
	20	ATCC6320	174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191	Perfectly matched	20
	22F	KD01-23	7, 8, 44, 195, 196, 197, 198, 199, 200	5 probes of group 22F/22A did not hybridized and 1 extra probe of group 7F/7A hybridized	22F/22A
	23F	KD11-15	144, 145, 156, 193, 201, 202, 203, 204, 205, 206, 207	Perfectly matched	23F
	33F	ATCC10370	208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222	Perfectly matched	33F/33A/37
Other serotypes included in 23 groups	6A	MSC1943	38, 39, 40, 41, 42, 43	Perfectly matched	6A/6B
	7A	ATCC6307	44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58	Perfectly matched	7F/7A
	9A	ATCC8333	71, 72, 73, 74, 75, 76, 77, 78, 84, 85, 81, 82, 83	Perfectly matched	9A/9V
	9L	ATCC10349	71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83	Perfectly matched	9L/9N
	11D	SSI11D/1	101, 102, 103, 104, 105, 106, 107, 108, 109	Perfectly matched	11A/11D
	12A	SSI12A/5	110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 274	Perfectly matched	12F/12A/12B/44/46
	12B	SSI12B/1	110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 274	Perfectly matched	12F/12A/12B/44/46
	15C	SSI15C/2	125, 128, 131, 135, 138, 139, 140, 141, 142, 143	Perfectly matched	15B/15C
	18B	ATCC10355	156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166	Perfectly matched	18B/18C
	22A	ATCC10363	7, 48, 49, 192, 193, 194, 195, 196, 197, 198, 199, 200	Perfectly matched	22F/22A
	33A	ATCC8340	208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222	Perfectly matched	33F/33A/37
	44	SSI44/3	110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 274	Perfectly matched	12F/12A/12B/44/46
	46	SSI46/2	110, 111, 112, 113, 114, 115, 116, 117, 119, 120, 121, 122, 123, 124, 274	1 probe of group 12F/12A/12B/44/46 did not hybridized	12F/12A/12B/44/46
Serotypes not included in 23 groups	7B	ATCC10348	143, 155	Partial hybridization	Not included in 23 group
	7C	ATCC10350	none	None hybridization	Not included in 23 group
	10F	ATCC6310	86, 87, 88	Partial hybridization	Not included in 23 group
	10B	SSI10B/2	71, 72, 73, 74, 78, 79, 80, 81, 82, 83	Partial hybridization	Not included in 23 group
	10C	SSI10C/2	71, 72, 73, 74, 75, 76, 77	Partial hybridization	Not included in 23 group
	11F	ATCC6311	103, 104, 105, 106, 107, 108, 109	Partial hybridization	Not included in 23 group
	11B	SSI11B/2	101	Partial hybridization	Not included in 23 group
	11C	ATCC10353	101, 274	Partial hybridization	Not included in 23 group
	15F	ATCC6315	101, 129, 131, 135, 141, 142, 143	Partial hybridization	Not included in 23 group
	15A	ATCC6330	101, 129, 131, 135, 141, 142, 143	Partial hybridization	Not included in 23 group
	17A	SSI17A/2	none	None hybridization	Not included in 23 group
	18F	ATCC6318	144, 145, 156, 157, 158, 159, 162, 163, 164, 165, 166, 193	Partial hybridization	Not included in 23 group
	18A	ATCC10344	144, 145, 156, 158, 160, 161, 162, 164, 165, 166	Partial hybridization	Not included in 23 group
	19B	ATCC10358	72, 167, 168,169, 171, 172	Partial hybridization	Not included in 23 group
	19C	ATCC10359	72,169,171,172	Partial hybridization	Not included in 23 group
	23A	KD12-06	144, 146, 156, 201, 202, 203, 204, 205, 206, 207	1 probe of group 23F did not hybridized	23F
	23B	ATCC10364	7, 46, 202,	Partial hybridization	Not included in 23 group
	33B	ATCC10342	none	None hybridization	Not included in 23 group
	33C	ATCC8339	none	None hybridization	Not included in 23 group
	33D	SSI33D/2	49, 57	Partial hybridization	Not included in 23 group

Explanatory notes: ^aThe numbers correspond to the spot identifiers given in Tables 2, 3, and Figure 1(a).