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Journal of Biomedicine and Biotechnology
Volume 2011 (2011), Article ID 394730, 5 pages
http://dx.doi.org/10.1155/2011/394730
Research Article

Rabbit Model of Retinoblastoma

L.F. Montgomery Ophthalmic Pathology Laboratory, Department of Ophthalmology, Emory Eye Center, Emory University School of Medicine, 1365-B Clifton Road NE, Atlanta, GA 30322, USA

Received 14 September 2010; Accepted 9 December 2010

Academic Editor: Monica Fedele

Copyright © 2011 Shin Jeong Kang and Hans E. Grossniklaus. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We created a rabbit model of retinoblastoma and confirmed the tumor clinically and histopathologically. Seventeen New Zealand rabbits were immunosuppressed with cyclosporin A at doses of 10–15 mg/kg. At day 3, the animals received a 30 μl subretinal injection of 1 × 1 0 6 cultured WERI retinoblastoma cells. Digital fundus images were captured before euthanasia, and the eyes were submitted for histopathology. Retinoblastoma cells grew in all the inoculated eyes and established a tumor under the retina and/or in the vitreous. New blood vessels in the tumor were observed starting at week 5. Cuffs of viable tumor cells surrounded the blood vessels with regions of necrosis present at 70–80 μm from nutrient vessels. Occasional tumor seeds in the vitreous histologically exhibited central necrosis. This rabbit model demonstrated similar fundus appearance and pathologic features to human retinoblastoma and may be used as a model to test various routes of drug delivery for retinoblastoma.